African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Identification and characterization of 16 recent Chinese isolates of bovine viral diarrhea virus genotype 2 (BVDV-2)

Tao J., Zhu l. Q., Lin Y. Q. and Zhu G. Q.*
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Email: [email protected], [email protected]

  •  Accepted: 01 March 2011
  •  Published: 04 June 2011

Abstract

 

Eighty calf blood samples were collected from different areas of China and were examined. Sixteen non cytopathic isolates of bovine viral diarrhea virus genotype 2 (BVDV-2) were isolated by specific BVDV-2-directed RT-PCR and sandwich ELISA. The results were verified by the indirect immunofluorescence assay and the ultra-thin sections. Genes of 5'-UTR, E2 and Npro from all the 16 field isolates were sequenced. The sequence identities at the nucleotide and amino acid levels were higher than 99.0%. The phylogenetic analysis revealed that the BVDV-2 USA strain, AzSpin, had the highest sequence homology with each of the Chinese isolates.

 

Key words: Bovine viral diarrhea virus genotype 2 (BVDV-2), isolation and characterization, phylogenetic analysis.