African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Evaluation of microbial elicitors to induce plant immunity for tomato wilt

Soytong Kasem1*, Charoenporn Chamaiporn2 and Kanokmedhakul Somdej3
1Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Ladkrabang, Bangkok, 10520, Thailand. 2Department of Agriculture, Faculty of Science and Technology, Nakhonratchsima Rajabhat University, Thailand. 3Natural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Email: [email protected]

  •  Accepted: 26 April 2013
  •  Published: 07 May 2013

Abstract

The bioactive compounds of Chaetoglobosin C, chaetomanone A and trichotoxin mixture used as microbial elicitors to elicit a-tomatine in tomato was investigated. The a-tomatinewas detected from tomato seedlings var. Sida sprayed with chaetoglobosin C, chaetomanone A and trichotoxin mixture, followed by the inoculation with Fusarium oxysporum f sp lycopersici. Results show that disease immunity on treated tomato seedlings with chaetoglobosin C, chaetomanone A and trichotoxin mixture at concentration 50 ug/ml after 10 days were 44.97, 35.18 and 39.43%, respectively, whileprochloraz showed disease immunity of 29.95%. The stems and leaves of tomato were extracted and spotted on TLC yielded green spot which showed Rf = 0.23 as same as a spot of standard a-tomatine. Tomato extracts were subjected into vial and analyzed for a-tomatine in HPLC system. Tomato treated with chaetoglobosin C, chaetomanone A and trichotoxin mixture at 15 days expressed a-tomatine quantity as 207.87, 254.25, and 205.04 ug/g which significantly were higher than for prochloraz and the inoculated controlwhose a-tomatine quantity were 131.56 and 77.46 ug/g, respectively. It showed that chaetoglobosin C, chaetomanone A and trichotoxin mixture could induce a-tomatine in tomato plants implying disease immunity against Fusarium wilt of tomato var. Sida through phytoalexin production. The bioactive compounds were tested for their efficacies to induce immunity in tomato wilt in vivo. The results reveal that all bioactive compounds at concentration 10, 50 and 100 µg/ml could induce plant immunity in tomato between 53.80-65.15% which significantly had higher plant disease immunity than prochloraz (26.73%) indicating that these bioactive compounds play an important role as microbial elicitors.

 

Key words: Chaetoglobosin C, chaetomanone A trichotoxin mixture, a-tomatine, microbial elicitors, tomato wilt.