African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Enzymatic activity, surface hydrophobicity and biogenic amines production in lactic acid bacteria isolated from an artisanal Spanish cheese

L. González Arias, D. Fernández, N. Sacristán, R. Arenas, J.M. Fresno, E. Tornadijo*
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de León, 24071 León, Spain
Email: [email protected]

  •  Accepted: 19 April 2013
  •  Published: 07 May 2013


The enzymatic activities of 24 lactic acid bacteria strains have been studied. Lactobacilli strains exhibited the highest β-galactosidase and aminopeptidase activities. Acid phosphatase activity was high in lactobacilli and lactococci strains. Esterase activity was observed for leuconostoc and lactobacilli strains. The hydrophobicity test and the biogenic amines detection were also tested in some strains selected for their use as autochthonous starters in cheese manufacture. In general, all strains showed degrees of hydrophobicity greater than 70%, and were poor former of histamine and cadaverine and medium putrescine former. Tyramine production was medium in three strains and low in two strains of Lactococcus.


Key words: Autochthonous starters, cheese, lactic acid bacteria, biogenic amines, enzymatic activity.


BA, Biogenic aminesMRS, Man-Rogosa SharpeHPLC, high performance liquid chromatography