African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Recombination study using Radish leaf curl virus isolates

  J. Kumar1,2*, S. P. Singh1, A. Kumar2, J. A. Khan2 and R. Tuli1
  1National Agri-Food Biotechnology Institute, Mohali -160071, Punjab, India. 2Molecular Virology Laboratory, National Botanical Research Institute, Rana Pratap Marg, Lucknow -226001, India.
Email: [email protected]

  •  Accepted: 03 July 2013
  •  Published: 05 July 2013



Begomovirus isolates, exhibiting identity of 89 and 93% with Radish leaf curl virus(RaLCV), were detected from the leaf curl disease affected okra (Abelmoschus esculentus) plants in Bihar, India. The detected isolates are supposed to be a new strain of RaLCV and therefore are referred to here as RaLCV-Hajipur1 and RaLCV-Hajipur2. Alphasatellite was identified as Cotton leaf curl Burewala alphasatellite (CLCuBuA) and betasatellite as Tomato leaf curl Bangladesh betasatellite (ToLCBDB). The aforementioned RaLCV-Hajipur isolates were used to construct infectious clones. These isolates were inoculated separately or together with alpha- and betasatellites in tobacco. The begomovirus and satellites were isolated from tobacco plants at twenty seven day post inoculation and then sequenced. Analysis of the sequences revealed the presence of a new isolate, referred to here as RaLCV-Hajipur3. Recombination analysis using recombination detection program 3 (RDP3) revealed that the RaLCV-Hajipur1, RaLCV-Hajipur2 and RaLCV-Hajipur3 had recombinant origin. RDP3 analysis of the RaLCV-Hajipur3 revealed that the two inoculated isolates (RaLCV-Hajipur1 and RaLCV-Hajipur2) were the probable major and minor parents of the newly detected isolate (RaLCV-Hajipur3).


Key words: Begomovirus, betasatellite, alphasatellite, recombination, evolution.