Eukaryotic expression plasmid (PVAX 1/E0) of E0 gene was constructed from an isolated strain of bovine viral diarrhea virus (BVDV) from sika deer and transfected into the BHK-21 cells to detect E0 gene transcription expression level and protein expression level. It was found that E0 gene could express in BHK-21 cells by RT-PCR and ELESA results. The humoral and cellular immune response levels were detected in rabbits immunized with PAVX1/E0 and inactivated vaccine by ELESA technology, which reached the peak after 42 days immunization and PVAX1/E0 immune rabbits could produce specific higher cells immune responses level in comparison to inactivated vaccine group. This is the first report demonstrating and “immunogenicity” of BVDV E0 gene on “sika deer”, indicating a new vaccine target for BVDV.
Key words: Sika deer, bovine viral diarrhea virus, E0 gene, eukaryotic expression, immune response.
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