African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Induced oxidative stress by Metarhizium anisopliae spp. instigates changes in lipid peroxidation and ultra structure in Periplaneta Americana

  Naren Babu Mutyala* and Padmaja Vadlamani
Department of Botany, Andhra University, Visakhapatnam- 530 003, India.
Email: [email protected], [email protected]

  •  Accepted: 26 August 2013
  •  Published: 20 September 2013



Consequent to injection of conidia at LC30, LC50 and LC90 doses of the entomopathogenic fungal isolates of Metarhizium anisopliae (Metch.), alterations in lipid peroxidation and ultrastructural changes were observed in cockroaches (Periplaneta americana). A decrease in the level of lipid peroxidation was evident in the treated cockroaches compared to those of control. Present investigation reports the effects of fungal infection on the midgut ultrastructure of the adult cockroaches which was manifested in the form of damage to the microvilli, epithelial cell vacuolization, necrosis and disruption of the epithelial cell membrane which occurred with increase in the time interval post treatment with conidia from high virulent isolate (M20) at LC50 dosage. Our study reveals for the first time that fungal infection instigates oxidative stress in the cockroach and that the villi of the midgut are also the target organs for the oxidative damage.


Key words: Entomopathogenic fungi, lethal concentration, cockroach, mid gut villi.


Abbreviations: ROS, Reactive oxygen species; SOD, superoxide dismutase; CAT,catalase; POX, peroxidase; APOX, ascorbate peroxidase; SDAY, Sabouraud dextrose agar with yeast extract medium; TBARS,  thiobarbituric acid-reactive substances; TCA,trichloro acetic acid; MDA, malondialdehyde; TEM, transmission electron microscopic;SEM, scanning electron microscopy; TMB, tetramethylbenzidine.