African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Development of PCR method for fast detection of Ophiostoma floccosum in wood chips

 Yeo Hong Yun, Dong Yeon Suh, Seung Yeol Son and Seong Hwan Kim*
Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714, Korea
Email: [email protected]

  •  Accepted: 26 April 2013
  •  Published: 07 May 2013


Ophiostoma floccosum is one of blue stain fungi that cause cosmetic damage in softwood. To develop a molecular maker for the detection of O. floccosum, we designed Oflo1 and Oflo2 primers based on the β-tubulin gene. PCR assay with eleven isolates ofO. floccosum and nine other blue stain and discoloring fungal species (moulds) proved that the designed primers specifically amplified a 333 bp sized DNA band only from genomic DNA of O. floccosum isolates. Together with microwave heating extraction of spore DNA, we could detect O. floccosum within 2 h on artificially inoculated wood chip. We believed that the developed PCR method will be useful where detection of target species such as O. floccosum is required.


Key words: Ophiostoma floccosum, Species-specific primer, β-tubulin gene.