African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Expression of chitinase and chitin binding proteins (CBP’s) by Listeria monocytogenes J0161 in biofilm and Co-culture broths

Prem Saran Tirumalai and Soam Prakash*
Environmental and Advanced Parasitology Lab, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute (Deemed University), Dayalbagh, Agra – 282110, India.
Email: [email protected]

  •  Accepted: 17 October 2011
  •  Published: 09 December 2011



Listeria monocytogenes is a dreaded pathogen colonizes to form biofilm in the natural environments and in food processing facility. Biofilm state makes the bacterium resistant to antimicrobial agents. L. monocytogenes, an intracellular parasite also colonizes the mammalian epithelial cells in the gut. Chitinase and other chitinolytic proteins expressed by L. monocytogenes contribute to the colonization of the bacterium in the intestine. It has been reported that chitinase and chitin binding proteins are essential for attachment of the bacterium to the epithelial cells. In this study, we have pursued to analyze, if chitinase and chitin binding proteins play a role in biofilm formation and the expression of these proteins otherwise in a co-culture state. We report the expression pattern of these proteins, which supports our claim that chitinase and chitin binding proteins have a role to play in biofilm formation.


Key words: Listeria monocytogenes, chitinase, microarray, biofilm, co-culture, gene expression.



CF, Cystic fibrosis; TSB, tryptone soy broth; 
PBS, phosphate buffer solution; DNA, deoxyribonucleic acid.