African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Optimize culture conditions for biomass and sporulation of the nematophagous fungus Pochonia chlamydosporia, isolate HSY-12-14 by “two-step” cultivation.

Li Gao
State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China.
Email: [email protected]

  •  Accepted: 07 July 2011
  •  Published: 23 November 2011



Biomass yields and sporulation of Pochonia chlamydosporia isolate HSY-12-14 was concerned on culture conditions including culture method, nutritional requirements together with environmental factors in this study. We optimized them for biomass yields ofP. chlamydosporia HSY-12-14 with the “two-step” cultivation method as well as orthogonal matrix method: firstly cultured spore suspension on the basal medium (sucrose 19.00 g, soy peptone 4.06 g, K2HPO4 1.00 g, KCl 0.50 g, MgSO4 0.50 g, FeSO40.01 g and 17.00 g Bactor) for the first stage culture of 4 days under room condition, then transferred them to another defined medium (sucrose 19.05 g, soypeptone 0.01 g,ZnSO4?7H2O  0.05 g/L, MnSO4?H2O  0.05 g/L, H3BO4 0.05 g/L and 17.00 g Bactor) for more 4 days cultivation, together with the environmental factors combination of water potential -3.9 MPa /pH 4 /24 h light cycle/23°C for biomass yields. We optimized the best culture conditions for sporulation of P. chlamydosporia under water potential -1.2 MPa /pH 4 /24 h light cycle/32°C with the same nutrition for biomass yields. These results is characterized in that it supplies the composition of media and environmental suitable for the mass production and provide important information industrialization of this great potential biocontrol fungus.


Key words: Nutrition, environment, sporulation, biological control, mass selection.