African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Microorganisms isolated from surgical wounds infection and treatment with different natural products and antibiotics

  Amany S. Youssef1*, Suzan A.M. El Feky2, Samy A. El-Asser1 and Rasha A.M. Abd Allah1
  1Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt. 2Department of Microbiology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Email: [email protected]

  •  Accepted: 16 July 2013
  •  Published: 26 July 2013



Surgical site infections (SSIs) are common nosocomial infections in surgical patients resulting in significant increases in postoperative morbidity and mortality. This study aimed to isolate and identify bacteria that cause SSIs in Medical Research Institute, Alexandria University, Egypt, and compare their sensitivities to selected group of antibiotics and natural products. Isolates from 20 patients with SSIs were identified by culturing on blood and MacConkey agars, Gram staining and biochemical reactions. Most Gram-negative isolates (Pseudomonas aeruginosa, Klebsiella spp, Acinetobacter spp) were sensitive to amikacin, imipenem. Gram-positive isolates were Enterococcus faecalis which was sensitive to chloramphenicol, vancomycin, and Staphylococcus aureus which was sensitive to amikacin, imipenem, chloramphenicol. Gram-negative isolates were more sensitive to olive oil at 70 and 100% concentrations, and Acinetobacter spp were resistant at 30% concentration. Gram-positive E. faecalis isolates were most sensitive at 30% concentration. Garlic and oregano oils were more effective against most Gram-positive and negative isolates. E. faecalis was resistant to garlic oil at 30% concentration.Klebsiella spp were resistant to Nigella sativa oil at all concentrations, Acinetobacter sppwere resistant at 30% concentration, and P. aeruginosa was more sensitive at all concentrations than Gram-positive isolates. P. aeruginosa and S. aureus were most sensitive, while Klebsiella spp were resistant to commercial oils (olive, garlic, and chamomile). Olive leaf extract was more effective at four concentrations against all Gram-negative isolates. Gram-positive and -negative isolates were most sensitive to whey mixed with honey. This study refers to the possibility of using olive, garlic and oregano oils, olive leaf extract, and whey mixed with honey in treatment of SSIs.


Key words: Isolated bacteria, surgical wounds, natural herbal products.