African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Screening and identification of tannase-producing fungi isolated from Brazilian caves

Alessandra Gonçalves de Melo1, Patrícia Nirlane da Costa Souza1, Natália da Costa Maia1, Ariela Betsy Thomas2, Leandro Buffoni Roque da Silva1, Luís Roberto Batista2, Rodrigo Lopes Ferreira3 and Patrícia Gomes Cardoso1*
1Department of Biology, Sector Microbiology, Federal University of Lavras, Lavras, Brazil. 2Department of Food Science, Federal University of Lavras, Lavras, Brazil. 3Department of Biology, Sector Zoology, Federal University of Lavras, Lavras, Brazil
Email: [email protected]

  •  Accepted: 02 January 2013
  •  Published: 05 February 2013

Abstract

Tannase is an extracellular inducible enzyme of great biotechnological interest. The microorganisms such as species from the Aspergillus and Penicillium genus are the most important source of tannase. The objective of this study was to isolate, identify and select strains of filamentous fungi present in caves located in the Brazilian biome for tannase production. Five hundred and forty-four fungal strains were isolated and three hundred and eighty-six had the ability to grow in plates containing tannic acid medium as the sole carbon source. A total of 32 strains were considered to be good tannase producers. Morphological characterization indicated 20 Aspergillus and 12 Penicilliumspecies. The highest tannase activity in submerged fermentation was obtained byAspergillus japonicus 246A (16.45 U/mg) and Aspergillus tamarii 3 (12.95 U/mg).

 

Key words: Aspergillus, cave, filamentous fungi, screening, submerged fermentation,tannase