African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

In vitro evaluation of antimicrobial activity of methanolic extract from selected species of Cephalopods on clinical isolates

  Pasiyappazham Ramasamy1, Namasivayam Subhapradha1, Alagiri Srinivasan2, Vairamani Shanmugam1, Jayalakshmi Krishnamoorthy1 and   Annaian Shanmugam1*  
  1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University Parangipettai – 608 502, India. 2Department of Biophysics, All India Institute of Medical Science, New Delhi – 110029, India.
Email: [email protected]

  •  Accepted: 30 July 2011
  •  Published: 23 October 2011



Bioactive substances from marine biota have been found useful as special tools in pharmacological and biomedical research. In the present study, the in vitro antimicrobial activity of crude methanolic extracts of six species of cephalopods (Sepia kobiensis,Sepiella inermisSepioteuthis lessoniana, Octopus aeginaOctopus aerolatusOctopus dollfusi) from Cuddalore (Southeast coast of India) was studied. The antimicrobial activity was screened against 10 species of clinically isolated human pathogenic bacteria namelyVibrio chlolerae, Pseudomonas aeruginosa, Klebsiella pnemoniae, Vibrio alginolyticus, Staphylococcus aureus, Vibrio parehaemolyticus, Streptococcus sp., Streptococcus pnemoniae, Salmonella sp. and Escherichia coli and five fungal strains such as Alternaria alternata, Candida tropicalis, Pencillium italicum, Fusarium equisetii and Candida albicans. Different concentrations such as 25, 50, 75 and 100% were prepared and tested against the microbial strains for their inhibitory activities, using the disc diffusion method. The minimum inhibitory concentration (MIC) of methanolic extract of cephalopods ranged from 60 to 100 mg/ml. The results were discussed in the light of positive and negative control apart from the concentrations tested.


Key words: Antimicrobial activity, cephalopods, minimum inhibitory concentration (MIC), pathogenic microorganisms.