African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Slime producing, heavy metals and antibiotics resistance in Aeromonas hydrophila isolated in Tunisia

Nourhene Saidi*, Rihab Lagha, Fethi Ben Abdallah, Karima Bekir Rokbani and Amina Bakhrouf
Laboratory of Analysis, Treatment, and Valorization of Environment and Products Pollutants, Department of Microbiology, Faculty of Pharmacy, Street Avicenne, Monastir, Tunisia.
Email: [email protected]

  •  Accepted: 20 May 2013
  •  Published: 31 December 2013


Aeromonas hydrophila strains isolated from different naturally polluted environments (ten from wastewater, six from bay used for aquaculture, eight from sea coast water and six from fish) were subjected to 13 antibiotics, and to four heavy metals (Copper, Cobalt, Zinc and Mercury) by using agar diffusion and agar dilution methods, respectively. In addition, effect of heavy metals on slime production was also investigated. Results of the antibiotic resistance agreed with those of heavy metals resistance, however, treated wastewater and bay strains were much tolerant than seawater and fish bacteria. The range of metal concentrations that was tolerated in the liquid media yielded information on the tolerance levels of A. hydrophila to different tested concentrations of metals. Copper and zinc were the best tolerated metals. Mercury was the most toxic component for all bacteria. Almost all A. hydrophila produced slime and a small number of strains have changed their morphotype under the heavy metals concentration. Our results have shown that Tunisian aquatic biotopes have a significant proportion of antibiotic and heavy metal resistant to A. hydrophila.


Key words: Antibiotic resistance, Aeromonas hydrophila, heavy metals and slime producing.