African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5242

Full Length Research Paper

Identification of diesel-tolerant rhizobacteria of Scirpus mucronatus

Ipung Fitri Purwanti1, Siti Rozaimah Sheikh Abdullah2, Hassan Basri1, Muhammad Mukhlisin1, Mushrifah Idris3 and Mohd Talib Latif4
1Department of Civil and Structural Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, UKM Bangi,Selangor, Malaysia. 2Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia. 3Tasik Chini Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia. 4School of Environmental Science and Natural Resources, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia.
Email: [email protected], [email protected]

  •  Accepted: 13 February 2012
  •  Published: 16 March 2012

Abstract

In this study, rhizobacteria were isolated from the roots of Scirpus mucronatus grown in diesel-contaminated soil. In a range finding test, microorganisms were isolated from soils contaminated with diesel in concentrations of 0, 10, 20, 100, and 200 g/kg. The number of colonies isolated was 38. After morphological and chemical tests, the number of bacteria colonies isolated was reduced from 38 to 35 bacteria. Of these, 23 bacterial isolates survived on 9 and 15% diesel-spiked mineral salt medium (MSM) after 5 days of exposure. Vitek® 2 Compact (France) identified three species of bacteria that could survive in the diesel-spiked plates, namely, Sphingomonas paucimobilis, Rhizobium radiobacter, andBacillus pumilus.

 

Key words: Rhizobacteria, diesel-tolerant, Scirpus mucronatus, phytoremediation, range finding test.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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