African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Effect of DLC-1 gene on tumour formation in nude mice inoculated with OVCAR-3

Liu Huina1,2, Shi HuiRong1*, Zhang HaiLing1, Wu KaiYuan1, Zhang RuiTao1 and Huang HaoLiang1
  1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, 450052, PR China. 2Department of Obstetrics and Gynecology, The Zhengzhou Central Hospital, 450007, PR China.
Email: [email protected]

  •  Accepted: 24 August 2011
  •  Published: 09 November 2011



The aim of the present study was to examine effect of DLC-1 gene transfect OVCAR-3 cells in vivo on tumour growth in nude mice. Results showed that tumor development rate in OVCAR-3/DLC-1 group, OVCAR-3/pEGFP-C3 group and OVCAR-3 group were 87, 87 and 100%, respectively. After 40 days, tumour cells were not detected in peripheral blood of mice in every group. Low expression of P-FAK, P- P130 cas and P-JNK proteins was detected in group OVCAR-3/DLC-1, while high expression was found in group OVCAR-3/pEGFP-C3 and OVCAR-3. the brightness of the bands of P130cas mRNA, FAK mRNA and JNK mRNA in group OVCAR-3/DLC-1 became weaker, while the brightness of the bands become stronger in group OVCAR-3/pEGFP-C3 and group OVCAR-3. DLC-1, as a anti-tumor related target gene, possesses a promising future.


Key words: DLC-1, ovarian cancer, nude mice, transfection.