The aim of the present study was to examine effect of DLC-1 gene transfect OVCAR-3 cells in vivo on tumour growth in nude mice. Results showed that tumor development rate in OVCAR-3/DLC-1 group, OVCAR-3/pEGFP-C3 group and OVCAR-3 group were 87, 87 and 100%, respectively. After 40 days, tumour cells were not detected in peripheral blood of mice in every group. Low expression of P-FAK, P- P130 cas and P-JNK proteins was detected in group OVCAR-3/DLC-1, while high expression was found in group OVCAR-3/pEGFP-C3 and OVCAR-3. the brightness of the bands of P130cas mRNA, FAK mRNA and JNK mRNA in group OVCAR-3/DLC-1 became weaker, while the brightness of the bands become stronger in group OVCAR-3/pEGFP-C3 and group OVCAR-3. DLC-1, as a anti-tumor related target gene, possesses a promising future.
Key words: DLC-1, ovarian cancer, nude mice, transfection.
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