African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Nucleotide excision repair and photoreactivation in sugarcane endophyte Gluconacetobacter diazotrophicus strain PAL5

Luc F. M. Rouws1*, Carlos H. S. G. Meneses2, Adriana S. Hemerly3 and José I. Baldani1
1Embrapa Agrobiologia, Seropédica, RJ, Brazil. 2Departamento de Agroecologia e Agropecuária, Centro de Ciências Agrárias e Ambientais / Programa de Pós-Graduação em Ciências Agrárias (MCA), Universidade Estadual da Paraíba, Lagoa Seca, PB, Brazil. 3Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Email: [email protected]

  •  Accepted: 06 November 2013
  •  Published: 28 November 2013


Nucleotide excision repair is a DNA repair mechanism mediated by three proteins encoded by the uvrABC gene homologues. This study shows that the mutant B12 of the diazotrophic sugarcane endophyte Gluconacetobacter diazotrophicus, carrying a transposon insertion in a uvrC homologue is hypersensitive to ultraviolet C irradiation. The exposure of irradiated mutant cells to visible light partially reverts the hypersensitivity, indicating that photoreactivation is another DNA repair mechanism active in G. diazotrophicus. Accordingly, a photolyase encoding gene is present on its genome. Therefore, this study brings experimental evidence that nucleotide excision repair and photoreactivation are DNA repair mechanisms in G. diazotrophicus.


Key words: Nitrogen fixing bacteria, endophytic bacteria, DNA repair, nucleotide excision repair, photolyase.


NER, nucleotide excision repair; UVC, ultraviolet C