African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Evaluation of activity of dichloroxylenol (1.2% w/v) on Staphylococcus aureus Oxoid 701/1 Lot 610254 and clinical isolates of Escherichia coli and Salmonella typhi

  Grillo, Joseph Adebayo* and Ojo, Oluwaseun Yinka
Department of Microbiology, Lagos State University, Ojo, P. M. B 0001, Ojo, Nigeria.
Email: [email protected]

  •  Accepted: 25 June 2013
  •  Published: 16 August 2013



The activity of dichloroxylenol (1.2% w/v), a commercial disinfectant formulation called Tiscol, on Staphylococcus aureus Oxoid 701/1 Lot 610254 and clinical isolates ofEscherichia coli and Salmonella typhi was carried out using the kill kinetics and the Kesley – Sykes procedures. The study demonstrates that the effectiveness of dichloroxylenol (1.2% w/v) against the test bacteria was time and concentration dependent and markedly affected by the presence of organic matter. Dichloroxylenol (1.2% w/v) also showed slightly greater activity against S. aureus Oxoid 701/1 Lot 610254 than against Escherichia coli and Salmonella typhi, indicating a probable role for cell wall in the susceptibility of bacteria to dichloroxylenol (1.2% w/v). The kesley – Sykes results suggest that in conditions of high contamination or presence of organic matter such as food, blood or tissue particles, dichloroxylenol (1.2% w/v) at or above 6% v/v can be appropriate in disinfection.   


Key words: Dichloroxylenol (1.2% w/v), Kill kinetics, Kesley – Sykes method.