African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Use of starter cultures of lactic acid bacteria and yeasts as inoculum enrichment for the production of gowé, a sour beverage from Benin

Vieira-Dalodé G*, Madodé Y. E 1, Hounhouigan J.1, Jespersen L.2 and Jakobsen M.2
1Département de Nutrition et Sciences Alimentaires, Faculté des Sciences Agronomiques, Université d'Abomey-Calavi, 01 BP 526 Cotonou, Bénin. 2Department of Dairy and Food Science, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK- 1958 Frederiksberg C, Denmark.
Email: [email protected]

  •  Accepted: 21 July 2008
  •  Published: 31 July 2008

Abstract

Lactobacillus fermentum, Weissella confusa, Kluyveromyces marxianus andPichia anomala, previously isolated during natural fermentation of traditional gowé, were tested as inoculum enrichment for controlled fermentation of gowé. The final product was subjected to chemical analysis and sensory evaluation. Growth of the lactic acid bacteria (LAB) and yeasts were verified by determination of colony forming units (CFU) and molecular biology techniques. A significant decrease in pH from 6.1 to 3.3, with a concomitant increase in titratable acidity (11 to 60 g/kg as lactic acid, dry weight), was observed after 24 h of fermentation when LAB was used either alone or in combination with yeasts. The LAB count increased significantly from 6.1 to 9.4 log CFU/ml, while the yeast count remained constant throughout fermentation. Repetitive-polymerase chain reaction (rep-PCR) assays performed on isolates during the fermentation confirmed the dominance of the added LAB strains. Sensory evaluation revealed that the product fermented for 7 h with L. fermentum alone or in combination with K. marxianus was as acceptable as the traditional product normally obtained after a minimum of 24 h of fermentation. Consequently, gowé can be obtained by controlled fermentation, using L. fermentum as inoculum enrichment, in a small scale industry.

 

Key words: Sorghum, Gowé, fermentation, starter cultures, lactic acid bacteria, yeasts, inoculum enrichment.