African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Bioaccumulation of cadmium and lead by Shewanella oneidensis isolated from soil in Basra governorate, Iraq

Raghad Jaafar
  • Raghad Jaafar
  • College of Education for Pure Science, Basra University, Basra, Iraq.
  • Google Scholar
Amin Al-Sulami
  • Amin Al-Sulami
  • College of Education for Pure Science, Basra University, Basra, Iraq.
  • Google Scholar
Asaad Al-Taee
  • Asaad Al-Taee
  • Marine Science Center, Basra University, Basra, Iraq.
  • Google Scholar


  •  Received: 09 January 2016
  •  Accepted: 26 February 2016
  •  Published: 28 March 2016

Abstract

In the present study heavy metals resistant bacteria were isolated from soil collected from Al-Zubair district in Basra governorate south of Iraq. On the basis of morphological, biochemical, 16S rRNA gene sequencing and phylogeny analysis, the isolates were authentically identified as Shewanella oneidensis in addition to Bacillus thuringiensis and Deinococcus radiodurans. The minimal inhibitory concentration (MIC) of isolates against cadmium (Cd) and lead (Pb) was determined on solid medium. S. oneidensis showed significant resistance to high concentrations of Cd (1000 mgl-1) and Pb (700 mgl-1). The bioaccumilation capabilities of S. oneidensis for Cd and Pb were monitored at different ion concentrations and contact times. The transmission electron microscope (TEM) study confirmed the accumulation of Cd and Pb by S. oneindensis causing morphological changes.

Key words: Shewanella oneidensis, bioaccumulation, minimal inhibitory concentration, heavy metals, transmission electron microscope.