African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Identification and partial characterization of antifungal and antibacterial activities of two Bacillus sp. strains isolated from salt soil in Tunisia

Ben Maachia Sihem1,2,4*, Errakhi Rafik3, Mathieu Florence1, Chérif Mohamed4, Lebrihi Ahmed1
1Laboratoire de Génie chimique UMR 5503 (CNRS/INPT/UPS), Institut National Polytechnique de Toulouse, 1, av. de l’Agrobiopole, 31326 Castanet-Tolozan, France. 2Centre Régionale de Recherches en Agriculture Oasienne, Dégache, Tozeur, Tunisia. 3Plateforme de biotechnologie AGRONUTRITION SAS 3, avenue de l'Orchidée Parc Activestre 31390 CARBONNE – France. 4Laboratoire de phytopathologie, Institut National Agronomique de Tunisie, Tunis, Tunisie.
Email: [email protected]

  •  Accepted: 10 May 2011
  •  Published: 04 July 2011


Two Bacillus sp. strains (B29 and B27) isolated from soil in the South of Tunisia were tested for their abilities to produce antimicrobial compounds. Both strains showed antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi. The produced compounds were extracted by using four different solvents. The hexane solvent allowed to obtain maximum of activity of the strain B29. The activity of the strain B27 was not elucidated by the four solvents used. Bio-autography results of B29 hexane extract revealed presence of different antibiotics and antifungal compounds with different Rf values of 0.3 and 0.76 for antifungal compounds and of 0.12, 0.14, 0.19 and 0.3 for antibacterial ones. Two active fractions were isolated from the culture broth of the strain B29 by semi-preparative high performance liquid chromatography (HPLC). The partial sequencing of the 16S rDNA gene was used to identify the two Bacillusstrains. They may be assigned to new Bacillus species.


Key words: Antimicrobial activity, Bacillus sp., characterization, salt soil, Tunisia.