Abstract

Amistar (Azoxystrobin 25%) and moncut (Flutolanil 25%) fungicides were used for treatment of faba bean plants in the field. Foliar application of the two fungicides by three doses exhibited a toxic effect on the total and individual fungal species associated with the roots and shoots of plant.  The inhibitive effect of these fungicides depended mainly on the fungicide concentration. The inhibitive effect increased with increasing concentration of fungicide at different periods of treatment. The ability of different fungal isolates to produce amylase enzymes was tested. Among 44 fungal isolates screened for amylase production, 19 isolates (43.2% of total isolates) exhibited high amylase activity. However 16 isolates (36.4% of total isolates) exhibited moderate amylase activity whereas the remaining 9 isolates (20.4% of total isolates) were low producers of amylase. When the two fungicides individually incorporated into the culture medium for amylase production (at 100 to 800 ppm) an inhibitive effect was exerted on the fungal growth and amylase production of Aspergillus flavus var. columnaris, Aspergillus tamarii, Penicillium chrysogenum and Penicillium funiculosum.

Key words: Fungicides, faba bean fungi, amylase enzymes.