African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Isolation and identification of microorganism from polyhouse agriculture soil of Rajasthan

Nakuleshwar Dut Jasuja1*, Richa Saxena1, Subhash Chandra1 and Suresh C. Joshi2
1Department of Biotechnology and Allied Sciences, Jayoti Vidyapeeth Women’s University, Jaipur- 303007, Rajasthan, India. 2Department of Zoology, University of Rajasthan, Jaipur-302004, India.
Email: [email protected]

  •  Accepted: 21 September 2013
  •  Published: 11 October 2013


The present work deals with the isolation and characterization of microorganisms from polyhouse agriculture soil of Jharna village (Rajasthan). Several bacteria and fungi were isolated from polyhouse soil, using serial dilution method. These bacterial isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Shigella sp., Proteus mirabilis, Bacillus anthracis, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis species which were further identified on the basis of colony morphology, Gram staining, biochemical tests and using selective and differential media. Identification of fungi such as Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Trichoderma sp. and Rhizopus sp. was carried out by culturing on potato dextrose and sabouraud’s dextrose agar media and microscopic method. Microorganisms play an important function in biodegradation of solid agriculture waste and also help in the crop production.


Key words: Bacteria, fungi, agriculture, polyhouse.