African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Evaluation of different selective media for enumeration of probiotic micro-organisms in combination with yogurt starter cultures in fermented milk

  Daniela Marques Saccaro1,Claudia Y. Hirota1, Adnan Y. Tamime2 and Maricê Nogueira de Oliveira1*
  1Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brasil. 224 Queens Terrace, Ayr KA7 1DX, Scotland, United Kingdom.
Email: [email protected]

  •  Accepted: 22 September 2011
  •  Published: 23 October 2011



The aim of this study is to assess selective plating methodologies for the enumeration and identification of Streptococcus thermophilusLactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilusLactobacillus rhamnosus and Bifidobacterium animalis ssp.lactis in fermented milks. Seven agar media (MRS with added sorbitol, clindamycin or vancomycin, acidified MRS, RCA with added aniline blue and dicloxacilin, M17 and ST) were evaluated. The results showed that RCA dicloxacilin agar is suitable for the selective enumeration of B. animalis ssp. lactis in fermented milkEither MRS (acidified) or M17 agar could be used for enumeration of L. delbrueckii ssp. bulgaricus and S. thermophilus,respectively. MRS media containing antibiotics were effective for the enumeration of the probiotic organisms (L. rhamnosus and L. acidophilus) inoculated in fermented milks.


Key words: Selective media, probiotic, starter cultures, fermented milk.