African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Red sea bream iridovirus infection in marble goby (Bleeker, Oxyeleotris marmoratus) in Taiwan

Ming-Hui Chen1,5, Shao-Wen Hung2,6, Chen-Hsuan Chang1, Po-Yang Chen1, Cheng-Chung Lin3, Tien-Huan Hsu1, Chin-Fu Cheng1, Shiun-Long Lin1, Ching-Yu Tu4, Chau-Loong Tsang1, Yu-Hsing Lin6 and Way-Shyan Wang1*
1Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung City 402, Taiwan. 2Institute of Brain Science, College of Medicine, National Yang-Ming University, Taipei 112, Taiwan. 3Graduate Institute of Veterinary Pathology, College of Veterinary Medicine, National Chung Hsing University, Taichung City 402, Taiwan. 4Residue Control Division, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Executive Yuan, Taiwan. 5The Livestock Disease Control Center of Taichung City, Taichung 402, Taiwan. 6Nursing Department of Yuanpei University, Hsinchu 300, Taiwan.
Email: [email protected]

  •  Accepted: 15 February 2013
  •  Published: 19 March 2013

Abstract

An outbreak of infectious disease in 30,000 marble gobies (Bleeker, Oxyeleotris marmoratus) cultured at a density of 5,000 to 8,000 fish per pond at a fish farm in the central Taiwan was reported to the Nantou County Animal Disease Center and the Central Taiwan Aquatic Disease Center. The mortality rate was approximately 40% (12,000/30,000). In May 2010, clinical signs of sluggish behavior, atypical swimming behavior at the edge of the pond and sudden death were noted among the marble gobies, which ranged from fry (12.7 cm) to juveniles (550 g). Moribund juveniles fish (n=5) were taken for diagnosis. Gross examination demonstrated cutaneous melanosis, local ulcers, and fatty liver. Gill imprint examined under light microscope revealed eggs of Dactylogyruscrucifer. Histopathology examination revealed inclusion body-like structures, necrosis, and hypertrophy in the pillar cells of gill, and multifocal proliferation of filament cells in gills. Additionally, lymphoid depletion, macrophages hyperplasia, and hypertrophy with the presence of intracytoplasmic vacuoles in the spleen as well as decreased numbers of melanomacrophages centers and hemocytoblasts in the kidney were observed. The result of polymerase chain reaction (PCR) of our case pathogen yielded a 570 bp fragment, which was consistent with the products of PCR obtained from red sea bream iridovirus (RSIV). The final diagnosis was RSIV infection in marble gobies. Additionally, antimicrobial agent sensitivity test showed no remarkable findings.

 

Key words: Fish, marble goby, red sea bream iridovirus, Taiwan