Abstract

The exopolysaccharide (EPS) of Lysinibacillus fusiformis were evaluated for their antioxidant properties. The total antioxidant capacity of EPS extracts was found to be maximum in malt medium 80.13 ± 0.26%. The hydrogen peroxide radical scavenging activity was found to be maximum in malt medium 78.3 ± 0.26%. The DPPH radical scavenging activity was found to be maximum in malt medium 48.39 ± 2.15%. 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) inhibition assay was found to be maximum in malt medium 50.75 ± 3.85%. This study suggested that the EPS from L. fusiformis could potentially be used for antioxidant activity. Agarose gel electrophoresis was used to find the structure of mobility using acetate buffer (pH 3.6) and citrate buffer (pH 9.0). The physiochemical characterization of EPS was studied and the structure was confirmed by the Fourier transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis.

Key words: Exopolysaccharide (EPS), Lysinibacillus fusiformis, Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC-MS).