African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

The role of various proteins of Toxoplasma gondii in provoking immune response at different stages of infection

Canan Kızılkaya1, Gülay Aral Akarsu1*, ÇiÄŸdem Güngör 1, Kerem Yaman1 and Ali Murat Aksoy2
1Division of Medical Parasitology, Faculty of Medicine, Ankara University, Turkey. 2Department of Medical Microbiology, Faculty of Medicine, Ankara University, Turkey
Email: [email protected]

  •  Accepted: 08 October 2012
  •  Published: 31 January 2013


Toxoplasma gondii is the causative protozoan of toxoplasmosis which has a worldwide distribution among humans and warm-blooded animals. The diagnosis generally depends on serologic tests but the persistently high Immunoglobulin M (IgM) or low IgG avidity complicate diagnosis. It is essential to identify acute-stage-specific antigen to use in a single test for the definitive diagnosis of the acute disease, especially in pregnant women and immunocompromised individuals. In this study, we investigated the recognition of proteins of lysate antigen (LA) and excretory-secretory antigen (ESA) fractions of the virulent RH strain of T. gondii by the sera of patients who had recent or past infections and the sera of experimentally infected mice. As a result, no specific bands for discrimination of recent infection from the past one could be detected in our study but the results confirmed that proteins of approximately 20, 30 and 40 kDa were among the major targets for IgG responses during acute and chronic infection with T. gondii in humans.


Key words: Toxoplasma gondii, acute, antigen, immunoblotting, human, mice.


ESA, Excretory-secretory antigen; LA, lysate antigen