African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Variations in structural and plasmid profiles of starved Shigella in seawater

Ali Ellafi1,2,*,#, Rihab Lagha1,#, Najla Haddeji1, Karima Bekir1, Silvia M. González-Leiza2, Juan A. Ayala2 and Amina Bakhrouf1 #Authors contributed equally to this work.
1Laboratory of Analysis, Treatment and Valorization of Environment Polluants and Product, Faculty of Pharmacy, Monastir University (Tunisia). 2Centro de Biologia Molecular "Severo Ochoa", CSIC, Campus Cantoblanco, 28049, Madrid, Spain.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 18 October 2013


In this study, we incubated three strains of Shigella (Shigella sonnei (S1), Shigella boydii(S2) and Shigella flexneri (S3)) in seawater microcosms (at room temperature and at 4°C) for eight months and we studied the modification of the proteins secreted, cytosolicand inner membrane profiles and their composition in fatty acids. Plasmid profiles were also investigated. Plasmids profiles analysis showed that S. boydii (S2) and S. flexneri (S3) did not lose any of their plasmids. In addition, fatty acids composition of Shigella under stress conditions was modified. Proteins secreted, cytosolic and inner membrane of stressed bacteria was changed and these modifications were manifested by the appearance and/or disappearance of bands.


Key words: Shigella, seawater, starvation, plasmids, proteins, fatty acids.