October 2012
Tetracycline resistance determinants of heterotrophic bacteria isolated from a South African tilapia aquaculture system
Tetracycline-resistant bacteria are frequently isolated from aquaculture systems, where mobile resistance genes often transfer between bacteria associated with fish kept at high stocking densities. Bacterial isolates from an Oreochromis mossambicus (tilapia) aquaculture system (Stellenbosch, South Africa) were screened for their susceptibility to tetracycline. Genomic and plasmid DNA were used in PCR-RFLP...
October 2012
Frequency of nosocomial infections with antibiotic resistant Acinetobacter spp. in intensive care unit (ICU) patients
Acinetobacter is a Gram-negative coccobacillus and one of the most nosocomial infections. Acinetobacter spp resistant strains in the world have created health problems. The aim of this study was to determine the prevalence and associated risk factors for infections with multi drug resistance (MDR) of Acinetobacter spp. in patients admitted to the ICU of Shahid Mostafa Khomeini Hospital, Tehran,...
October 2012
Occurrence and antibiotic resistance of enterococci in ready-to-eat food of animal origin
The aim of this work was to determine which Enterococcus species could be isolated from food of animal origin and the significance of these enterococcal isolates according to their antibiotic resistance profiles. Ninety-two Enterococcus strains were isolated from retail food of animal origin in Olsztyn, Poland. They were classified as Enterococcus faecalis (44...
October 2012
Occurrence of fungal contamination in pistachio and peanut samples from retail shops in Sanandaj province, Iran
Aspergillus flavus produces secondary metabolites called aflatoxin that has powerful teratogenic, mutagenic and hepato-carcinogenic effects. The aim of this study was to evaluate the A. flavus of pistachio and peanuts from Sanandaj, Iran. Pistachio and peanut nuts samples were obtained from dried fruit retail shops of Sanandaj, 2011. One hundred grams from each sample were sterilized in a 0.4% sodium...
October 2012
Cellulase production by Aspergillus sp. on rice grass (Spartina spp.) under solid-state fermentation
In this study, the feasibility of using rice grass (Spartina spp.) as the main substrate for cellulase production by a newly isolated strain Aspergillus sp. SEMCC-3.248 under solid-state fermentation was evaluated. The optimized medium and culture conditions were: rice grass (0.8-0.9 mm) 2.5 g, Wheat bran 1.5 g, 4 ml of nutrient solution ((NH4)2SO4 14 g/L, KH2PO4 2 g/L, CaCl2 4 g/L,...
October 2012
Recognition and differentiation of various Penicillium species, Penicillium citrinum, Penicillium notatum, Penicillium oxalicum and Penicillium frequentans, using random amplified polymorphic DNA-polymerase chain reaction technique
Molecular methods have been established to make better sensitivity and specificity for differentiation of pathogenic and allergenic fungi. The purpose of this study was to obtain an appropriate molecular pattern for differentiation of Penicillium species understudy. In this study, 30 isolates of four Penicillium species including Penicillium citrinum,Penicillium notatum, Penicillium...
October 2012
Ethylenediaminetetraacetic acid: A non antibiotic adjuvant enhancing Pseudomonas aeruginosa susceptibility
Pseudomonas aeruginosa is an opportunistic bacterium which has been shown to have multi-drug resistance against fluoroquinolones, β-lactams, and aminoglycosides. In this investigation, we studied the effect of different concentrations of ethylenediaminetetraacetic acid (EDTA) on MexA-MexB-OprM efflux pump and subsequent changes in susceptibility and expression. Next, we examined, the expression...
October 2012
Detection of Brucellosis in sheep intended for export and local slaughter in Khartoum State, Sudan
This study is designed to determine the prevalence rate of brucellosis in both sheep for export and local consumption ones. Brucellosis was studied in 404 heads of sheep in Khartoum State, Sudan. 300 heads of them were prepared for export and 104 were for slaughter for local consumption. Rose bengal plate test (RBPT), serum agglutination test (SAT) and competitive enzyme linked immunosorbent assay (cELISA) were used in...
October 2012
Detection of uropathogenic Escherichia coli virulence factors in patients with urinary tract infections in Iran
Besides all human infectious diseases, urinary tract infections have a high prevalence and in the majority of cases Escherichia coli is a dominance bacterium which can cause pyelonephritis and cystitis. In this present study, a total of 123 strains of E. coli isolated from patients with urinary tract infections were tested in a polymerase chain reaction for detection of E. coli’s...
October 2012
Antimicrobial potentials of crude fractions of polysaccharides of Ganoderma spp.
The present study aimed to screen antimicrobial activity of polysaccharide extracts ofGanoderma on pathogenic microorganisms. Ganoderma spp. were collected from different parts of Mazandaran province, Iran, and identified using keys and morphological characters. From each Ganoderma spp. 4 fractions (I, II, IIIa and IIIb) were prepared and tested using well assay method against the target...
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