African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 13 May, 2015; 9(19)

May 2015

Dermatophytes: Diagnosis of dermatophytosis and its treatment

The enzymatic ability of fungi to decompose keratin has long been interpreted as a key innovation in the evolution of animal dermatology. Dermatophytes are keratinophilic fungi which were originally saprophytic, but have adapted themselves to animal and human parasitism during the course of evolution. Dermatophytes are pathogens, which cause superficial mycosis. The dermatophytes have the capacity to invade keratinized...

Author(s): Vishnu Sharma, Tarun Kumar Kumawat, Anima Sharma, Ruchi Seth and Subhash Chandra

May 2015

Alternative technique for culturing sputum for mycobacteria isolation: Feasibility, performance and effect on laboratory quality assessment - A technical note

Mycobacteria culture remains the cornerstone of tuberculosis diagnosis. Naturally contaminated samples need pre-inoculation processing but some economically challenged medical facilities may benefit from a simpler and cheaper sputum decontamination procedure. The aim of this study was to test a simple decontamination method lacking a centrifugation step to be used in conjunction with the culture on...

Author(s): Andrei-Tudor Cernomaz, Simona Péter, Adina-Catinca Gradinaru, Florin Rusu-Cordunean, Berlea Madalina Lavinia, Sabina-Ioana Cojocaru, Radu Zonda, Ionut Pavel, Ina–Iuliana Macovei and Bogdan-Dragos Grigoriu

May 2015

Effect of different temperature levels and time intervals on germination of uredospores of Puccinia sorghi

In plant disease development, temperature is a critical factor. To determine how specific environmental variables affect corn rust, we determined temperature effects on urediniospore germination. Spore germination was maximum within 24 h of incubation, in the temperature range of 20-30°C. The mean maximum spore germination was recorded at 25°C (66.16% germination) which is statistically on par at 30°C...

Author(s): Utpal Dey, S. I. Harlapur, D. N. Dhutraj, Dibakar Pal and D. V. Pawar

May 2015

Occurrence and antibiogram profile of Staphylococcus aureus isolated from some hospital environment in Zaria, Nigeria

Staphylococcus aureus is an opportunistic pathogen and is the most frequent cause of hospital acquired infection. A total sample of 310 was collected for this work; the pathogen was isolated from hands of some healthcare workers/nurses, operation tables, door knobs/door handles, nurses’ table tops, bedrails, stretchers, floors, toilets seats, cupboards, and sinks. The total percentage prevalence of the pathogen in...

Author(s): Hammuel, C., Idoko, M. O., Migap, H. H. and Ambrose, N.

May 2015

Identification, antibiotic resistance and distribution of different classes of integrons among proteus species isolated from different sources in Dakahleia and Damietta Egyptian Governorates

The aim of the present investigation is to trace the epidemiological relatedness between different isolates of proteus sp. obtained from Dakahleia governorate as compared with similar sources from the adjacent Damietta governorate in Egypt. A total of 500 bacterial isolates were collected from human and animal sources including urine samples, sputum, wound, throat and ear swabs, endotracheal aspirate, breast swab, bile...

Author(s): Mohamed Adel El-Sokkary, Mohamed Mohamed Adel El-Sokkary, Rasha Aabed and Rasha Barwa

May 2015

Colletotrichum gloeosporioides lipase: Characterization and use in hydrolysis and esterifications

Colletotrichum gloeosporioides was evaluated for its capacity to produce extracellular lipase. A crude enzyme preparation obtained after 48 h of fermentation reached 742 U/L of lipolytic activity. Estimated molecular weight of proteins responsible for this activity was about 18 kDa as determined by SDS-PAGE and zymogram analysis. Enzyme preparation showed optimum pH at 10 and stability at optimum temperature (37°C)...

Author(s): Denise Sande, Lívia Tereza A. Souza, Jamil S. Oliveira, Marcelo M. Santoro, Inayara Cristina A. Lacerda, Gecernir Colen and Jacqueline Aparecida Takahashi

May 2015

Pseudomonas fluorescens (Pf1) mediated chitinolytic activity in tomato plants against Fusarium oxysporum f. sp. lycopersici

Liquid formulation of Pseudomonas fluorescens strain Pf1 was found to protect tomato plants from wilt disease caused by Fusarium oxysporum f. sp. lycopersici (FOL). The lytic enzyme and chitinase activity were assayed in the tomato plants treated with liquid as well as talc based formulations of Pf1 and challenged with the FOL. The chitinase activity was significantly increased in tomato plants treated with sequential...

Author(s): R. Manikandan and T. Raguchander

May 2015

Seroprevalence and risk factors of small ruminant brucellosis in selected districts of Arsi and East Shoa zones, Oromia region, Ethiopia

A cross-sectional study was conducted in two districts in Arsi zone and one district in East Shoa zone, Ethiopia, to determine seroprevalence and assess the possible risk factors associated with small ruminant brucellosis. A total of 840 blood samples (409 sheep and 431goats) were collected. All sera samples were screened by modified Rose Bengal Test (mRBT) and all positive reactors were further tested by indirect...

Author(s): Abiot Deddefo, Tesfaye Sisay and Getachew Tuli