African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 14 June, 2012; 6(22)

June 2012

Active constituents of some Satureja L. species and their biological activities

Methanol extracts from the aerial parts of three Satureja L. spp. belonging to the family Lamiaceae were studied. S. cilicica is endemic to Turkey; S. icarica and S. coerulea extend to West Anatolia; S. icarica extends to the Turkish-Greek border and S. coerulea extends to the Turkish-Bulgarian border. S. icarica, S. coerulea, and S....

Author(s): Tulin Askun, Gulendam Tumen, Fatih Satıl and Didem Karaarslan

June 2012

Evaluation of indirect immunofluorescence assay in patients with autoimmune diseases

Antinuclear antibodies (ANA) are the hallmark of autoantibody production in autoimmune disease. ANA are directed against components of the cell nuclei such as DNA, histones, nucleoli and ribonucleoproteins. ANA detected by indirect immunofluorescence (IIF) is the method of choice and is regarded as the gold standard technique. 100 patients with autoimmune disease attending a tertiary care hospital at Chennai, India and...

Author(s): Sridhar S. and Vasanthy N.

June 2012

Gamma ray mediated mutagenesis of Phialocephala humicola: Effect on kinetics and thermodynamics of a-amylase production

The current report dealt with the development of the fungus Phialocephala humicolathrough gamma ray treatment for the hyper production of α-amylases, which have diverse industrial applications. Improvement of microbes for the over production of industrial products has been the hallmark of all commercial fermentation processes. Three mutants (M13, M16 and M24) were selected based on the hyper-production of...

Author(s): Tayyaba Huma, Muhammad Hamid Rashid, Muhammad Rizwan Javed and Asma Ashraf

June 2012

Molecular typing of Vibrio alginolyticus strains isolated from Tunisian marine biotopes by two PCR-based methods (ERIC and REP)

The aim of the present study was to analyze the intraspecific genetic variability within Vibrio alginolyticus isolated strains from different Tunisian aquatic biotopes by using two PCR–based techniques. Seventy eight strains including nine Vibrio reference strains and sixty nine V. alginolyticus isolated strains from seawater, sediment, fish (diseased Gilthead...

Author(s): Lajnef Rim, Snoussi Mejdi, Balboa Sabela, Bastardo Asmine, Laabidi Haifa, Chatti Abdelwaheb, Abdennaceur Hassen and Romalde Jesús Lopez

June 2012

Assessment of the antifungal activity of Nicotiana glauca Graham aqueous and organic extracts against some pathogenic and antagonistic fungi

In the present research work, Nicotiana glauca Graham was used as a potential source of biologically active compounds. The antifungal activity of leaf and flower aqueous and organic extracts (petroleum ether, chloroforme and methanol) was assessed in vitro against three phytopathogenic fungi and two antagonistic agents. These target fungi were subjected to the different types of extracts already...

Author(s): Asma Rinez, Mejda Daami-Remadi, Faten Omezzine, Afef Ladhari, Imen Rinezand Rabiaa Haouala

June 2012

Antibacterial and antifungal activities of Cotula coronopifolia

This study examines the antibacterial and antifungal activities of extracts, and one active principle: 6-methoxy-1-benzofuran-4-ol, isolated from the aquatic plant Cotula coronopifolia, against six pathogenic bacterial strains and seven pathogenic fungi. Results showed positive antibacterial and antifungal values, and underline the potential of the plant C. coronopifolia either as natural preservative or...

Author(s): Kaouthar Liouane, Karima Bel Hadj Salah, Houda Ben Abdelkader, Mohamed Ali Mahjoub, Mahjoub Aouni, Khaled Said and Zine Mighri

June 2012

Biotoxicity assay of Bacillus thuringiensis combined with sodium citrate, Bifenthrin+Cypermethrin and Saraca indica on Tribolium castaneum

Microbial control of insect pest of crops using entomopathogens is pest management strategy. Bacillus thuringiensis has a great potential for this purpose. The focus of the present study was to determine the individual and combined effect of different insecticides such as Saraca indica as a botanical insecticide, B. thuringiensis and chemical insecticides against larvae and adults...

Author(s): Kausar Malik, Sadaf Iqbal, Farkhanda Jabeen, Amjad Farooq, Shagufta Andleeb, Asmat Ullah, Mir Muhammad Ali Talpur and Hafeez Ikram

June 2012

Chemical composition, antibacterial and antifungal properties of Tunisian Nigella sativa fixed oil

We investigated the chemical composition (by gas chromatography) and anti-microbial activity of the Tunisian Nigella sativa fixed oil against different standard Gram-negative and Gram-positive strains and Candida isolates by disc diffusion method, and determined the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and minimal fungicidal concentration (MFC) values. Twenty three...

Author(s): Hanene Jrah Harzallah, Emira Noumi, Karima Bekir, Amina Bakhrouf  and Touhami Mahjoub

June 2012

Pulsed-field gel electrophoresis, plasmid profile and antimicrobial resistance pattern of Salmonella typhimurium isolated from human and retail meats

Salmonella enterica subspecies enterica serovar Typhimurium is one of the common food pathogens which may cause human disease and/or animal infections. In an attempt to trace the clonal relationship and to discern the possible transmission of these strains from different origins, twenty-five Salmonella typhimurium strains isolated from retail meats and four strains isolated from...

Author(s): Ines Abbassi-Ghozzi, Salah Hammami, Ridha Ben Aissa, Jaime Martinez-Urtaza,Abdellatif Boudabous and Maher Gtari

June 2012

Molecular study of bacteria associated with Salicornia symbiotic bacteria as a candidate for Hormozgan salty zone culturing by Persian Gulf water irrigation

Hormozgān is one of the 30 provinces of Iran. It is in the south of the country, facing Oman. Unfortunately, water extraction, in excess of the rate of replenishment, saline intrusion and inappropriate use of fertilizers have caused some increases in the salinity of many areas, which during the last two decades became a major problem in the production of traditional crops in many regions. Salicornia is a...

Author(s): Behboud Jafari, Morteza Hanifezadeh and Maryam Saadat Jalali Parvin

June 2012

Effect of protective culture and biopreservatives on strained yoghurt quality

In this research, the protective culture (Lactobacillus rhamnosus LC705 andPropionibacterium freudenreichii JS) and biopreservatives as Nisin (Nisaplin), Delvocid®(natamycin) or Nipasol M (propyl paraben) on the physical, chemical and sensory properties of strained yoghurt were studied in relation to its viability of undesirable yeasts-moulds during storage at 4±1°C for 28 days. Based on...

Author(s): Fatmaana MISIRLILAR, Özer KINIK and Oktay YERLÄ°KAYA

June 2012

Prevalence and antibiotic resistance of Listeria species in food products in Taipei, Taiwan

A total of 400 samples including meat products, dairy and dairy products, fresh vegetables, fresh seafood, and ready-to-eat food products from supermarkets in Taipei area were collected and analyzed for the prevalence of Listeria species. The overall occurrence ofListeria spp. was 16.5%, and most of them were isolated from meat products and vegetables. Listeria monocytogenes was isolated...

Author(s): Feng-ing Wang, Ming-kai Chern, Chi-wang Li, Min Yan, and Yu-hsien Hsieh,

June 2012

Effects of different vegetable oils formulations on temperature tolerance and storage duration of Beauveria bassiana conidia

The aim of this work was to evaluate the effects of different vegetable oil formulations on the temperature tolerance and storage duration of Beauveria bassiana conidia. The germination ability of conidia mixed with eight vegetable oils including rapeseed, soy, sesame, corn, coconut, grape, olive and almond oils, were evaluated for the conidia temperature tolerance at 25, 30, 35, 40 and 45°C by spreading...

Author(s): F. Lotfi Mola and R. Afkari

June 2012

Soil biochemical dynamics at three elevations during the soil thawing period, Eastern Tibetan Plateau: Nutrient availabilities, microbial properties and enzyme activities

Studies during last decade have established that soil microbial biomass, enzyme activity and nutrient pools in some cold regions often keep relatively high level in winter and drop rapidly when soil temperatures approach and exceed 0°C during winter-spring transitional period. However, the inconsistent results were observed in different ecosystems due to various temperature dynamics. Based on previous observations...

Author(s): Bo Tan, Fu-zhong Wu, Wan-qin Yang, Ao Wang and Yu-lian Yang

June 2012

Survey of hygiene in ovine slaughterhouses of Algiers region by bacteriological analysis of carcasses

Ovine meat is a major protein source in Algeria. Hygiene of food stuffs is an important concern in food production since food poisoning is a danger for public health. Two slaughterhouses were investigated in this study. Ten carcasses were sampled in each slaughterhouse during 4 consecutive weeks. The amount of total aerobic mesophilic flora and of Enterobacteriaceae was calculated on the 80 carcasses. The results were...

Author(s): Harhoura Kh, Boukhors K. T., Dahmani A., Zenia S. and Aissi M.

June 2012

Sensitivity of ruminal bacterial isolates of sheep, cattle and buffalo to 13 therapeutic antibiotics

Ruminal bacterial isolates, 59 from two sheep, five cows and nine buffaloes were used to evaluate sensitivity to the therapeutic antibiotics amikacin, cefadroxil, cefoperazone, cefotaxime, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, piperacillin, polymyxin, roxithromycin, streptomycin and vancomycin. Sensitivity of ruminal bacterial isolates to each was determined by the clearance zone (CZ) in the...

Author(s): Abdel Fattah Zeidan Mohamed Salem, Yosry Mahmoud Gohar,  Secundino Lopez, Manuel Gonzalez-Ronquillo and Maria Andrea Cerrillo

June 2012

Extracellular trehalose production by Micrococcus lylae and its enhancement by heat shock

We examined the effects of heat (50°C), osmotic (3% NaCl), and solvent (3% ethanol) shock on the extracellular trehalose production by Micrococcus lylae KCTC 3517 in glucose-supplemented medium. Cultivation of M. lylae at 37°C for 12 h followed by heat shock of exponentially growing cells at 50°C for 2 h at 12 h-intervals resulted in maximum trehalose production (1.72 mg/ml), which...

Author(s): Myung-Ji Seo, Wook-Dong Kim and Soon-Ok Kim

June 2012

Optimization of extraction parameters for protein from beer waste brewing yeast treated by pulsed electric fields (PEF)

In order to improve the permeability of yeast cell membrane, pulsed electric fields (PEF) was applied in extracting protein from beer waste brewing yeasts (BWBY). Through investigation of three independent variables, including electric field strength (10 to 50 kV/cm), pulse numbers (2 to 16 μs), and liquid-solid ratio (20:1 to 60:1), the optimal extraction parameters for protein were obtained by one-factor-at-a-time...

Author(s): Mingyuan Liu, Meishuo Zhang, Songyi Lin, Jingbo Liu , Yi Yang and Yan Jin

June 2012

Distribution of hepatitis C virus (HCV) genotypes in different remote cities of Pakistan

Hepatitis C virus (HCV) infection is a serious health problem that affects millions of individuals worldwide. About 85% of the HCV patients develop persistent HCV infection that leads to liver cirrhosis with chronic Hepatitis C. HCV genotypes have a diversified pattern of frequency and distribution throughout the world and are critically important in understanding the epidemiological problems. Hepatitis C genotype...

Author(s): Muhammad Faisal Bashir, Muhammad Saleem Haider, Naeem Rashid and Saba Riaz,

June 2012

Carbapenem-resistant and OXA-23 producing Acinetobacter baumannii isolate in Tunisian hospital

We investigated resistance determinant of clinical isolate of carbapenem–resistantAcinetobacter baumannii, collected from patients hospitalized at the Military hospital in Tunisia. The isolate which was multidrug resistant produced OXA-23. The bla OXA-23 gene was found to be adjacent to ISAbaI.   Key words: Acinetobacter baumannii, OXA-23, ISAbaI.

Author(s): Hela Chihi, Amel Bourouis, Sihem Mahrouki, Rym Ouertani, Farouk Barguellil, Mouhamed Ben–Moussa and Omrane Belhadj

June 2012

Effects of Lycium barbarum polysaccharides (LBP) on immune function of mice

This experiment was conducted to investigate the effects of Lycium barbarumpolysaccharides (LBP) on immune system function. The effects of different doses of LBP on the relative weight of immune organs, delayed type hypersensitivity, spleen lymphocyte proliferation reaction and NK cell activity in mice were investigated. LBP increased the relative weight of immune organs and improved cellular immune...

Author(s): Yi Nan, Rong Wang, Ling Yuan, and Yang Niu

June 2012

Folate, MTHFR C677T and A1298C polymorphisms with the relationship with ovarian cancer risk among Chinese females

Genetic and environmental factors may play roles in the pathogenesis of ovarian cancer; we aimed to evaluate the associations between MTHFR C677T and A1298C polymorphism and folate intake with ovarian cancer. A case-control study with 200 ovarian cancer...

Author(s): Changxin Song, Lei Ping and Wang Ting

June 2012

Use of starter cultures of Bacillus and Staphylococcus in the controlled fermentation of Lanhouin, a traditional fish-based condiment from West Africa

Strains of Bacillus subtilis, Bacillus licheniformis and Staphylococcus xylosus previously isolated during the spontaneous fermentation of Lanhouin were tested singly and in combination for their ability to ferment pasteurized fish flesh to produce a new type ofLanhouin, a traditional condiment product. The growth of viable cells during the fermentation was monitored according to...

Author(s): Victor B. Anihouvi, Euloge Y. Kpoclou and Joseph D. Hounhouigan

June 2012

Brucellosis among ruminants in some districts of Bangladesh using four conventional serological assays

Brucellosis causes a great economic loss to the livestock industries through abortion, infertility, birth of weak and dead offspring, increased calving interval and reduction of milk yield and it is endemic in Bangladesh. The present study was performed to know the seroprevalence of brucellosis for 1000 ruminants (135 Buffaloes, 465 cattle, 230 goats and 170 sheep) in five different districts of...

Author(s): Md. Siddiqur Rahman, Moon Her, Ji-yeon Kim, Sung-il Kang, Kichan Lee, Muhammad Jasim Uddin, Amitavo Chakrabartty and Suk Chan Jung

June 2012

In vitro and in vivo antitumoral activitiy of sodium dichloroacetate (DCA-Na) against murine melanoma

Normal cells typically use mitochondrial oxidative phosphorylation to metabolize glucose and switch over to glycolysis only in hypoxic or anoxic environment. However, cancer cells avidly consume glucose for energy by glycolysis to survive in the hypoxic environment of malignant lesions, a phenomenon known as the Warburg effect, the glycolytic phenotype offers growth advantages to cancer cells by resisting apoptosis....

Author(s): Franco-Molina M. A., Mendoza-Gamboa E., Sierra-Rivera C. A., Zapata-Benavides P., Miranda-Hernández D. F., Chávez-Reyes A., Rivera-Morales L. G., Tamez-Guerra R., and Rodríguez-Padilla C.

June 2012

Diversity of organism in the Usnea longissima lichen

Lichens are self-supporting and ecologically obligate associations between symbiotic fungi, commonly an Ascomycete (mycobiont), and a photosynthesising organism (photobiont). Samples of Usnea longissima lichen were collected from Abies georgei in the Pudacuo National Park, Shangri-la County, Yunan Province, China, and their biological compositions were analysed by cultural and metagenomic approaches. We found...

Author(s): Yunzhe He and Zhiguo Zhang

June 2012

Morphological and molecular identification of pathogenic fungal of post-harvest tomato fruit during storage

Three pathogenic fungi of tomato were separated by using transconjugate method and identified by morphological characteristics and internal transcribed spacers ribosomal DNA (ITS rDNA) sequence analysis. Morphological studies showed that FQ1, FQ2 and FQ3 generated spores on potato dextrose agar (PDA) plates and slides. The ITS rDNA regions of these pathogens were amplified by polymerase chain reaction (PCR) and then...

Author(s): Guofang Xie, Shuming Tan and Lu Yu

June 2012

Antibacterial potential of Withania somnifera L. against human pathogenic bacteria

The present study was conducted to evaluate the antibacterial activity of chloroform, acetone, methanolic, and ethanolic crude extracts of stem, leaves, and roots of Withania somnifera. It was observed that acetone extracts was the most effective followed by methanolic and ethanolic extracts for all the test organisms used. It was observed that acetone extracts were the most effective in inhibiting the growth...

Author(s): Humaira Rizwana, Amal Abdulaziz Al Hazzani, Afaf Ibrahim Shehata and Nadine Mohamed Safouh Moubayed

June 2012

Incidence and virulence of Bacillus cereus isolated from Egyptian foods during four seasons

The incidence of Bacillus cereus in many food products including beef luncheon, koshary, karish cheese, double white cream cheese, white salty cheese and corn snacks was tested during four seasons. Generally, there was a high incidence rate during summer in all food samples and this rate decreased during winter. The incidence of B. cereus was high in karish cheese (25 to 80%), koshary (45 to 70%) and...

Author(s): Gamal Enan, Nadia Awny, Azza A. Abou Zeid and Mohamed Ahmed Abdou

June 2012

Effects of microelement fertilizers and phosphate biological fertilizer on some morphological traits of Purple coneflower in water stress condition

In order to study the effects of microelement fertilizer and phosphate biological fertilizer “Phosphate Biofertilizer” on some morphological characteristics of Echinacea purpurea  under water stress, an experiment was carried out using split-factorial method on the base of RCBD with three replicates in the agricultural research station of  Islamic Azad University, Tabriz Branch, Iran....

Author(s): Mehrdad Yarnia, Maryam Farzanian, Varahram Rashidi, Aziz Javanshir and Naser Aliasgharzad