African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 18 March, 2011; 5(6)

March 2011

Feasibility of using dot blot hybridization to detect Salmonella InvA, SpiC and SipC directly from clinical specimens

  Pathogenesis of Salmonella depends upon a large number of factors controlled by an array of genes that synergise into actual virulence. The goal of this study was to detectSalmonella invA, spiC and sipC directly from clinical specimens, using the dot blot hybridization assay. We detected invA, spiC and sipC as a one combination from 4.5% (95% CI:...

Author(s): Hang’ombe Bernard Mudenda, Ulaya Williama, Mwansa James C. L., Mubita Charles, Isogai Nayuta, Mulenga Evans, Moonga Ladslav, Isogai Hiroshi and Isogai Emiko

March 2011

Bioethanol fuel production from rotten banana as an environmental waste management and sustainable energy

  Banana waste is discarded due to the imperfection during grading process. Banana biomass can be used as raw material to produce bioethanol. In this study, fermentation of banana waste was conducted using Saccharomyces cerevisiae, Type II under anaerobic condition. Production of bioethanol was determined and the effects of various operating conditions which included different temperatures, shaking period,...

Author(s):   Hossain, A. B. M. S., Ahmed, S. A., Ahmed M. Alshammari, Faris M. A. Adnan, Annuar, M. S. M., Hadeel Mustafa and Norah Hammad

March 2011

Isolation and characterization of heavy metal removing bacterial bioflocculants

  Thirteen metal-tolerant bacteria capable of producing metal-removing bioflocculants were isolated from an industrial effluent sample. Pseudomonas sp. was found to be the pre-dominant species among the isolates (8 out of 13), followed by Herbaspirillium spp. (4) and one Paenibacillus sp. The flocculating activity of bioflocculants produced by these microorganisms was assayed using...

Author(s): J. Lin and C. Harichund

March 2011

Antimicrobial screening of selected medicinal plants in Tamilnadu, India

  The present study deals with the antimicrobial activity of the aqueous, acetone and petroleum ether extracts of the leaves, stem and root of Andrographis ovata Clarke, Aristolochia indica L., Eclipta prostrata L. and Gloriosa superba L., using agar diffusion method against human pathogens, such as Escherichia coli, Proteus vulgaris, Psudeomonas...

Author(s): C. Alagesaboopathi

March 2011

The activity and integrated service for microbial resources at the Korean agricultural culture collection

  The Korean agricultural culture collection (KACC) has developed a web-based system to provide an integrated database about microbial resources containing 12,924 registered cultures of microorganisms. This system- service platform consists of three major functions, namely microorganism search, ordering and deposit. In 2009, we newly registered 3,039 microbial...

Author(s):   Chang Kug Kim, HangYeon Weon, GyuTaek Cho, Soon-Wo Kwon, SooChul Park and Seung Beom Hong

March 2011

Application of phytodisinfectants in water purification in rural Cameroon

  Findings from a preliminary lab-scale study show strong potentials of phytodisinfectants as a low-cost, appropriate and ecological alternative technology in purifying water in rural Cameroon. A survey of plants used in water purification in Bamenda, Cameroon, indicated that there are many plants used in water treatment. A rapid screening on the coagulative and disinfection potential of four most frequently...

Author(s): K. A. Yongabi, D. M. Lewis and P. L. Harris

March 2011

Antibacterial effects of Oradex, Gengigel and Salviathymol-n mouthwash on dental biofilm bacteria

  Three mouthwashes Gengigel, Oradex and Salviathymol N, were tested using experimental microorganisms included Fusobacterium nucleatum, Streptococcus mitis,Streptococcus constellatus, Eikenella corrodens and dental plaque. Staphylococcus aureus and Escherichia coli were used as internal controls. Antibacterial activity was done by diffusion test. Minimum inhibitory...

Author(s): F. H. AL-Bayaty, T. B. Taiyeb-Ali, M. A. Abdulla and Z. B. Mahmud

March 2011

Fibrolytic potential of spent compost of Agaricus bisporus to degrade forages for ruminants

  To evaluate the fibrolytic potential of spent compost of champignon mushroom (Agaricus bisporus; SCAB) on forage degradation, liquids from spent compost were extracted and then evaluated in vitro. Firstly, SCAB was recovered at 50, 60 and 90 days post-harvest and enzyme activity was evaluated. Celluloses activity was highest at 60 days; xylanases activity was not affected by post-harvest...

Author(s): Ayala, M., González-Muñoz, S. S., Pinos-Rodriguez, J. M., Vázquez, C., Meneses, M., Loera, O. and Mendoza, G. D.

March 2011

Increasing the bioethanol yield in the presence of furfural via mutation of a native strain of Saccharomyces cerevisiae

  High ethanol yield is a desired property of industrial yeast strains. A significant problem in fermentative conversion of lignocellulosic materials to ethanol is the formation of furfural and hydroxymethylfurfural (HMF), which are formed during acid hydrolysis of lignocellulose. Furfural has been known to create strong inhibition in metabolism of the yeast Saccharomyces cerevisiae. In this article,...

Author(s): Bahareh Rahimian Zarif  Mehrdad Azin  and Nour Amirmozafari 

March 2011

Screening of Pogostemon parviflorus Benth. for anti-Candida activity

  Pogostemon parviflorus, an aromatic woody herbaceous plant, is found in high rainfall localities. The medicinal properties of this plant show that leaves are used for anxiety, cellulite, depression, eczema and wounds. Also, they are used for thrush, mycotic enteritis and vaginitis. The aim of the present study is to assay the anti-Candida activity of the ethanolic and methanolic...

Author(s): Shahla Najafi and Batool Sadeghi-Nejad

March 2011

Continuous butyric acid production by corn stalk immobilized Clostridium thermobutyricum cells

  Corn stalks were used as a support to immobilize Clostridium thermobutyricum in a fermentation process for butyric acid production. The effects of pH and acetic acid concentration on butyric acid production were examined in a steady-state 25-day continuous flow operation. A metabolic shift was induced by changing pH of the medium. The maximum and the minimum ratio of butyric acid and acetic acid...

Author(s): Wei Li, Hui-Juan Han and Chun-hui Zhang

March 2011

Combination of Dichelobacter nodosus LAMP with Fusobacterium necrophorum LAMP for detecting hoof swabs from dairy cattle footrot

  To investigate the presence of Fusobacterium necrophorum (F. necrophorum) and Dichelobacter nodosus (D. nodosus) in dairy cattle footrot, a loop-mediated isothermal amplification method for detection of D. nodosus (Dn-LAMP) was developed and evaluated using species-specific16S rRNA gene as target gene. The Dn-LAMP showed no cross reaction with several common pathogens from cattle, and...

Author(s): Sun D. B., Wu R., Guo D. H., He X. J., Zheng J. S., Wang J. F., Lin Y. C., Han X., Wang Y. Q. and Guo T. T.

March 2011

Antibiotic drug resistance of hospital acquired Staphylococcus aureus in Andra Pradesh: A monitoring study

  Nosocomial infections are one of the occupational biohazards that affect the health of individuals with or without predisposing factors. Staphylococcus aureus is associated with significantly higher mortality and is associated with community-acquired serious nosocomial infections because strains generally show multiple drug resistance, which limits treatment possibilities. A total of 1800 patients...

Author(s): Rabbani Syed, Giri Prasad, Farha Deeba, Rani D. Kaiser Jamil, and Ali A. Alshatwi 

March 2011

Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus strains isolated from Turkish homemade yoghurts

  The aim of this study was to determine the inhibitive effect of 25 Lactobacillus delbrueckissp. bulgaricus and 16 Streptococcus thermophilus strains isolated from 30 different homemade yoghurts on several pathogen and contaminant bacteria. The antibiotic resistance of these bacteria was also determined. All of Lactobacillus bulgaricus strains exhibited antimicrobial activity...

Author(s): Asli Akpinar, Oktay Yerlikaya and Sevda Kiliç

March 2011

Prevalence, antimicrobial resistance and associated risk factors for bacteriuria in diabetic women in Dar es Salaam, Tanzania

  Urinary tract infections (UTIs) occur frequently among women with diabetes. The present study aimed at determining prevalence and risk factors of bacteriuria in diabetic women and antimicrobial resistance pattern of the isolates at Muhimbili National Hospital (MNH), Dar es Salaam. Three hundred diabetic women attending clinic at MNH from June to November 2010 were included in the study. Demographic and...

Author(s): Eligius F. Lyamuya, Sabrina John Moyo, Ewaldo V. Komba and Martin Haule

March 2011

Poly-β-hydroxybutyrate (PHB) production from domestic wastewater using Enterobacter aerogenes 12Bi strain

  A strain of poly-β-hydroxybutyrate (PHB)-accumulating bacterium was isolated and identified as Enterobacter aerogenes (designated E. aerogenes 12Bi) by using biochemical and phylogenetic characterization. The accumulation of a large amount of granules in its cells cultured in the domestic wastewater medium (DWWM) were showed by transmission electron microscopy (TEM). When...

Author(s): Nur Ceyhan and Guven Ozdemir

March 2011

Genetic analysis of Karakul sheep breed using microsatellite markers

  In this study, the genetic variation in Karakul sheep was investigated using 15 microsatellite markers (MCMA2, BMS460, BM1815, OARCP26, OARFCB20, MAF64, OARAE129, BMS332, LSCV38, BM6444, BMS995, MCMA26, BMS678 and OARCP49) and all fifteen loci were amplified successfully. Genomic DNA was extracted from 120 blood samples, using modified salting-out method. Tests of genotype frequencies for deviation from the...

Author(s): Shahram Nanekarani, Cyrus Amirinia and Nour Amirmozafari

March 2011

Sensitive and specific detection of Agrobacterium tumefaciens in soil using a rapid polymerase chain reaction (PCR)

  One pair of primers was designed based on the sequence of tmr locus for specific and sensitive detection of Agrobacterium tumefaciens. Only the A. tumefaciens strain can produce the 236bp target fragment among the fourteen bacterial species that tested. The sensitivity of the specific PCR system was determined by a nested-PCR amplification which can numbered the copies of the template...

Author(s):   Wei Yang, Lei Ji, Li-Rong Tan, Shi-Mo Li, Yan Wang, Hong-Xia Liu, and Yu-Ming Luo

March 2011

Effects of arbuscular mycorrhizal (AM) fungi Glomus mosseae on characteristics of leaf development of Paeonia suffruticosa under salt stress

  The effects of Arbuscular mycorrhizal (AM) fungi on the characteristics of leaf development of Paeonia suffruticosa at different levels of salt stress (0, 8, 16 and 24%) were studied. Potted ‘Feng Dan’ seedlings were inoculated with Glomus mosseae, and the non-inoculated was used as the control. The results showed that under salt stress leaf relative water content,...

Author(s): Guo Shaoxia,  Han Tingting and Liu Runjin

March 2011

Complement C1, C3 and C4 levels in sera of leishmaniasis cases in Turkey

  Leishmaniasis is still a problem for many countries including developed ones. The subgroups of the serum complements have significant roles on the onset of the infection. The aim of this study was to determine the serum complement (C1, C3, C4) levels of cases with definite diagnosis of leishmaniasis and compare them with healthy controls. The study group included 43 visceral leishmaniasis (VL) and 13...

Author(s): Limoncu M. E., ÅžanlidaÄŸ T., BalcioÄŸlu I. C., Akçali S., Özensoy S. and Özbel Y.

March 2011

A study on comparative pharmacological efficacy of Berberis lycium and penicillin G

  The importance of medicinal plants in traditional healthcare practices provides opportunity to new areas of research. However, information on the uses of plants for medicine is lacking from many interior areas of District Mansehra. Keeping this in view, the present study was initiated. This study attempts to shade lights on the comparative pharmacological efficacy of Berberis lycium (medicinal...

Author(s): Ihtisham Bukhari, Mukhtiar Hassan, Fida M. Abbasi, Ghulam Mujtaba, Nasir Mahmood, Noshin, Anees Fatima, Muhammad Afzal, Mujaddad Ur Rehman, Farzana Perveen and M. Tariq Khan

March 2011

Antimicrobial potential of Xylaria polymorpha (Pers.) Grev.

  The ethanolic extracts obtained from the macrofungus Xylaria polymorpha (Pers.) Grev. (Ascomycetes) have been investigated for their antimicrobial activity. Growth inhibition using agar disc diffusion assays was determined against Escherichia coli ATCC 11230, S. aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus...

Author(s): Nurcihan Hacioglu, Ilgaz Akata and Basaran Dulger

March 2011

Detection of Entamoeba histolytica with different methods admitted to the emergency department in diarrheic patients

  Antigen screening were conducted to stool samples from 60 patients admitted to our emergency department with diarrhea complaint between June 2009 and October 2009 by the methods of direct microscopic examination, trichrome staining, ELISA (Enzyme-linked immunosorbent assay), respectively. As a result of examination of total 60 samples with direct microscopic, trichrome staining and ELISA...

Author(s): Ozlem Aycan Kaya, Hakan Oguzturk, Muhammet Gokhan Turtay and U. Nilgun Daldal

March 2011

E-test versus disk diffusion method in determining multidrug resistant strains of Escherichia coli in urinary tract infection

    Multidrug resistant strains of Escherichia coli are becoming a major challenge in treatment of patients with urinary tract infection. Developing effective screening methods for selection of antimicrobial resistant strains is necessary. In this study, we have compared descriptively results of E-test with Disk diffusion agar method in selection of multidrug resistant strains of E. coli...

Author(s):  Y. Erfani , A. Rasti , A. Mirsalehian , S. M. Mirafshar , V. Ownegh

March 2011

Antimicrobial activities of the whole plant of Cestrum nocturnum against pathogenic microorganisms

  The crude methanol extract of the whole plant of Cestrum nocturnum L. (Solanaceae) and its  subsequent fractions were tested against various bacterial and fungal strains. With the exception of  Salmonella typhi, the tested samples showed marked antibacterial activity against Pseudomonas  aeruginosa, Staphylococcus aurous, Bacillus subtilis, Escherichia coli and Shigella flexenari....

Author(s): Murad Ali Khan , Humaira Inayat , , Haroon Khan , , Mohammmad Saeed , Ikhtair Khan    and Inayat-Ur-Rahman