November 2012
Aptamer biosensor for Streptococcus hemolyticus detection based on fluorescence quenching by gold nanoparticles
This study describes a nanodiagnostic method using specific aptamer and gold nanoparticles (AuNPs) to detect Streptococcus hemolyticus. In this assay, fluorescein(FAM) labeled single-stranded DNA (ssDNA) probe was hybridized with the complement aptamer sequences which immobilized on the surface of AuNPs, and the fluorescence was quenched by AuNPs. However, when the aptamer DNA specifically combined...
November 2012
An atypical patient with 2009 influenza (H1N1) infection and multiple organ dysfunctions
In this study, we report a case infected with H1N1 influenza virus and developed complications of multiple organ dysfunction (MOD). Also, we describe in this work the clinical course and successful treatment of the patient. We discussed our experience in the diagnosis and treatment of the patient with an atypical disease. Key words: Influenza A, H1N1, Multiple organ dysfunctions.
November 2012
Identification of putative virulence-associated genes of Streptococcus pyogenes by genomic subtractive hybridization
The fragments of virulence genes in Streptococcus pyogenes were isolated with suppression subtractive hybridization (SSH). Then these fragments were directly inserted into T/A cloning vector to set up subtractive library, and the amplification of the library was carried out after transferring into Escherichia coli TOP10. Dot blot was used to screen the subtracted library, and the differentially...
November 2012
Mycoinsecticide effects of Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea on the whitefly Bemisia tabaci (Homoptera: Aleyrodidae) in different strata of bean
Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea are entomopathogenic fungi used as biological control agents for whitefly. They have been individually evaluated under laboratory, greenhouse and field conditions; however, there is little information on their comparative efficacy under greenhouse conditions particularly with respect to the height of the canopy in which they act. Mortality...
November 2012
Antimicrobial activities of secondary metabolites produced by endophytic bacteria from selected indigenous Kenyan plants
Endophytic microorganisms are to be found in virtually every plant on earth. These organisms reside in the living tissues of the host plant and do so in a variety of relationships, ranging from symbiotic to slightly pathogenic. Endophytes are a potential source of novel chemistry and biology products/compounds to assist in helping solve human health problems. In the present study, bacteria endophytes were...
November 2012
Isolation and biochemical characterization of bacterial isolates producing different levels of polygalacturonases from various sources
Sixty-one bacterial strains were isolated from various sources including soil, water, rotted fruits and vegetables. Preliminary screening for pectinase producing bacterial strains was done by well plate method. Sixteen bacteria giving zones from 08 to 22 mm were selected and subjected to cellular, morphological and biochemical characterization. Further screening of selected strains was done quantitatively...
November 2012
Carotenoid composition of Mucorales fungi
Mucorales are known as b-carotene producing fungi and some species, namely Blakeslea trispora, Phycomyces blakesleeanus and Mucor circinelloides, are used as model organisms in carotenogenic studies. However, little is known about the carotenoid production and composition of other species belonging to this fungal group. In this study, carotenoid content of 21 isolates representing 11 species and five...
November 2012
Microorganisms colonizing surface in coastal marine water as revealed by 16S rRNA gene clone library analysis
To investigate the community structure and dynamics of surface-associated microbial cells, sterile strengthened glass sheets were immersed in near-shore marine water of Xiamen, China. The organisms colonizing the glass surface were sampled after 1 h, 7 and 14 days. Three 16S rRNA gene clone libraries were constructed using primers universal for the domain bacteria. 40 clones were...
November 2012
Microbiological and chemical quality of Çökelek cheese, Lor cheese and Torba (strained) yoghurt
To assess the level of contamination of milk products such as, Çökelek cheese (n = 25), Lor cheese (n = 25) and Torba (strained) yoghurt (n = 25) samples were examined for microbiological and chemical quality. In Çökelek cheese samples, 96, 56, 56, 56, 24, 8, 8, 4, 4 and 44% of yeast and mould, total coliform, fecal coliform, Escherichia coli, Bacillus cereus, Clostridium...
November 2012
Effect of starch containing organic substrates on alpha amylase production in Bacillus strains
This study was conducted to identify and screen indigenous Bacillus strains from different soil samples for alpha amylase activity. Strains positive for the enzyme activity wereBacillus subtilis, Bacillus mycoids, Bacillus licheniformis and Bacillus cereus. These were further studied...
November 2012
Elevated levels of serum IL-32 in patients with active pulmonary tuberculosis
To explore interleukin-32 relationship with tuberculosis, we detected interleukin-32 expression level in the serum samples of patients with active tuberculosis. Forty (40) TB patients (16 males and 24 females) from Third People's Hospital of Kunming, China, and 17 healthy controls (10 males and 7 females) from the Center for Disease Control and Prevention of Kunming, China, were selected. An enzyme-linked...
Advertisement
Advertisement
