April 2013
Effect of garlic extract on the luciferase, bio-luminescence, virulence factors produced by Vibrio harveyi with a challenge during Penaeus monodon larviculture
There has been augmented curiosity in the application of herbal products against diseases causing marine pathogenic bacteria in aquaculture system. This paper describes antagonism of different types of garlic extract on the growth of Vibrio harveyithat produces luciferase, virulence and bio-luminescence. Aqueous filtered garlic extract (AFGE) showed zone of inhibition (12.33 ± 0.50 mm)...
April 2013
Effects of urea on length distribution and morphology of Escherichia coli and Salmonella enterica subsp. enterica cells
Our previous studies showed that urea in acidic broth induced swarmer morphotypes in Proteus mirabilis, but the effects to other bacterial species remain unsolved. Here we report effects of urea on morphology and size distribution of urease-negativeEscherichia coli and Salmonella enterica serovar Abony grown in urea-containing broth. Statistical analysis indicated lognormal distribution of the...
April 2013
Chemical composition, antimicrobial and antioxidant properties of the essential oils of Tulbaghia violacea Harv L.F.
Tulbaghia violacea Harv L.F. is one of the many medicinal plants used by Zulu traditional healers to treat respiratory tract diseases. Essential oil extracted from the rhizomes of T. violacea was evaluated for its chemical composition, antioxidant and antibacterial activities. The gas chromatography/mass spectrometry (GC/MS) analysis of the oils revealed the main constituents of the...
April 2013
Polygenic analysis of ammonia-oxidizing bacteria for completely autotrophic nitrogen removal
This study investigated the functional microbial community in a sequencing batch biofilm reactor (SBBR) for completely autotrophic nitrogen removal over nitrite (CANON), and analyzed the phylogenetic relationships of the key bacteria, the aerobic ammonia oxidizing bacteria (AOB), with three different markers genes: 16S rDNA, amoA and hao. The resulting tree topologies from all the markers were...
April 2013
Antibacterial activity of isolated human intestinal microbiota Lactobacillus strains against methicillin resistant and susceptible Staphylococcus aureus
In the present study, lactic acid bacteria were screened for anti-methicillin resistant activity. Isolation of lactic acid bacteria from 100 faecal samples of healthy adults was conducted using de Man-Rogosa Sharpe agar (MRS) and three Lactobacilli strains were isolated. The three isolates of human intestinal microbiota were identified on the basis of morphological and biochemical characteristics. The...
April 2013
Evidence for horizontal gene transfer of a tetrahydrofuran monooxygenase: Cloning and analysis of a gene cluster for tetrahydrofuran degradation in Rhodococcus sp. YYL
A gene cluster encoding tetrahydrofuran (THF) degrading related enzymes was first isolated from Rhodococcus sp. YYL, a strain of the genus Rhodococcus, which was first reported to degrade tetrahydrofuran, by combination of COnsensus-DEgenerate Hybrid oligonucleotide Primer PCR and thermal asymmetric interlaced PCR. Sequence analysis of the gene cluster revealed 9 open reading frames containing genes...
April 2013
Microorganisms involved in endodontic infection of permanent teeth: A systematic review
This study investigated the microbial species present in necrotic pulps of permanent teeth needing endodontic treatment. The search for articles was conducted on the Health Science Database and considered all articles published until October 2011, with selected human clinical studies examined, through molecular biology the microorganisms that are present in root canals of permanent teeth requiring root canal...
April 2013
Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India
Sclerotinia stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most serious disease and causes damage to oilseed Brassica. Seventeen geographical isolates of S. sclerotiorum from different geographical locations of India were compared for their growth under artificial culture conditions and the result was validated throughrandom amplified polymorphic DNA (RAPD) markers....
April 2013
Management of seed/soil borne diseases of safflower by chemical and biocontrol agents
An experiment was carried out at the Oil seed Research Unit, Marathwada Krishi Vidyapeeth Parbhani, Maharashtra, India to evaluate the efficacy of different chemicals and bioagents against seed/soil borne diseases of safflower on disease incidence (PI or DI). Significantly low disease severity (DS) and low disease incidence (DI) were recorded in treatment withT7, that is, Trichoderma harzianum Th4d sc at 2...
April 2013
Risk factors and outcome of Acinetobacter infection in the intensive care unit of a tertiary center in Oyo State, Nigeria
Acinetobacter plays an important role in the infection of patients admitted to hospitals.Acinetobacter are free living gram-negative coccobacilli that emerge as significant nosocomial pathogens in the hospital setting and are responsible for intermittent outbreaks in the intensive care unit. The aim of this study was to determine the risk factors and outcome of Acinetobacter infections in patients...
April 2013
Characterisation of Campylobacter concisus strains from South Africa using Amplified Fragment Length Polymorphism (AFLP) profiling and a Genomospecies-specific Polymerase Chain Reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data
Amplified Fragment Length Polymorphism (AFLP) profiling was used to evaluate the distribution of phenotypically indistinguishable, but genetically distinct, amongCampylobacter concisus strains from South Africa. A Polymerase Chain Reaction (PCR) assay described for identifying strains belonging to Genomospecies 1 and 2 was appliedin this study. Forty-seven C. concisus strains were studied in total,...
April 2013
The anti-oomycetic effects of sodium chloride and potassium permanganate and the toxicity of these compounds to tilapia (Oreochromis niloticus) eggs
The anti-oomycetic effects of sodium chloride and potassium permanganate on the vegetative and zoosporic stages of Achlya sp. BKKU 0502, Saprolegnia diclina BKKU 0506, Aphanomyces sp. BKKU 0508, Achlya ambisexualis BKKU 0615 and Achlya bisexualis BKKU 0616 were investigated at 25°C. The results showed that the exposure of the samples to 3.0 and 2.5% sodium chloride...
April 2013
Yeast selection for high resistance to and uptake of Se: Cultural optimization of organic selenium production
Se-rich yeast within animal feeds is much more effective than additions of inorganic Se in increasing the concentration of Se in eggs, milk and meat. This study was conducted in order to select mutant Saccharomyces cerevisiae which produce higher levels of organic selenium (Se) and to improve the productivity of this Se-rich yeast by optimization of the culturing condition. Among 13 ATCC strains of S....
April 2013
Bioefficacy of fungicides, bio-control agents and botanicals against leaf spot of turmeric incited by Colletortricum capsici
Efficacy of ten fungicides with three different concentrations, that is, 500, 1000 and 1500 ppm were studied in vitro against Colletortricum capsici by poisoned food technique. Results revealed that Propiconazole was significantly superior to all other fungicides by inhibiting (100%) growth followed by Hexaconazole (96.55%) and Chlorothalonil (86.78%) over control after 10 days of incubation....
April 2013
Isolation, identification and optimization of fermentation parameters for improved production of antimicrobial compounds from indigenous Streptomyces isolates
In order to find out new antimicrobial compounds producing Stretomycetes from indigneous soil, twenty five Streptomyces isolates were purified from hundred soil samples and screened for their antimicrobial potential. Among these isolates, four (SK-1, SK-3, SK-4 and SK-5) were selected for further studies on the basis of antimicrobial compounds produced by them. Morphological, cultural, physiological and ...
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