African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 30 January, 2012; 6(4)

January 2012

Ethanol production by Zymomonas mobilis PTCC 1718 using low cost substrates

We report our attempts to increase ethanol production from Zymomonas mobilis PTCC 1718, by optimization of the components of growth medium and culture conditions. First, low cost substrates, carbon and nitrogen sources, temperature and initial pH were optimized by one-factor-at-a-time method. Then, nutritional improvement was performed using Taguchi method. Amongst the different selected sources, sucrose and...

Author(s): Shiva Soleimani, Mohammad Faezi Ghasemi, and Soheil Shokri

January 2012

A glimpse of the yeast Saccharomyces cerevisiae responses to NaCl stress

The budding yeast Saccharomyces cerevisiae has been widely used in fermentation and brewing industries. In industrial fermentation processes, the raw material often attaches certain NaCl or Na+ into bioreactor. High salinity represents an osmotic stress and specific ion toxicity for yeast cell. Protective biochemical reactions range from the synthesis of osmolyte to altered ion transportation, signal...

Author(s): Hongyang Ren, Xinhui Wang, Dayu Liu and Bing Wang,

January 2012

Food borne diseases bacteria; frequency antibiotic resistance bacteria in Iranian foods

Food borne illnesses result from eating food or drinking beverages that are contaminated with chemicals matter, heavy metals, parasites, fungi, viruses and Bacteria. Bacillusspecies and Staphylococcus aureus are one of food borne diseases Bacteria. Bacillusspecies are spore forming bacteria and the spores may be present on raw foods, and their ability to survive high cooking temperatures requires...

Author(s): Shila Jalalpour

January 2012

Wheat bran as a brown gold: Nutritious value and its biotechnological applications

Wheat bran, a by-product of wheat milling industry, is the outermost covering of wheat grain. It has relatively diverse application in food, feed, medicine and fermentation industries due to its richness in carbohydrates (mostly fibers), protein and fats which makes it an important dietary element. Its importance from medical view point is well documented especially in reducing blood plasma cholesterol and prevention of...

Author(s): Muhammad Mohsin Javed, Sana Zahoor, Sarah Shafaat, Iffat Mehmooda, Ambreen Gul, Huma Rasheed, Syed Ali Imran Bukhari, Muhammad Nauman Aftab and Ikram-ul-Haq

January 2012

Construction and immunogenicity of a genetically mutant of a native Shigella dysenteriae type 1 isolated from an Iranian patient with diarrhea by recombineering

Since protective immunity against shigellosis is serotype specific, it appears that construction of a live Shigella dysenteriae type 1 strain (attenuated by deletion of the virGgene as a native new vaccine candidate) is urgently needed. The virG gene from a native of S. dysenteriae type 1 was amplified and cloned into the pGEM-5Zf vector and sequenced. Alignment analysis indicated that...

Author(s): M. Heiat, M. Saadati, S. Mostafa Hosseini, T. Zahraei Salehi, B. Nayeri Fasaei, Y. Ahsani Arani, A. R. Salehi, M. Doroudian and E. Hosseini

January 2012

Improvement of yield potential of rice through combined application of biofertilizer and chemical nitrogen

A field experiment was conducted to evaluate the suitable combination of plant growth promoting rhizobacteria (Azospirillum biofertilizer strain BM9 and BM11) along with different nitrogenous fertilizer levels (0, 20, 40, 60, 80 and 100% N) on rice variety Binadhan 4 at Bangladesh Institute of Nuclear Agriculture farm, Mymensingh in Aman season of 2006. Roots of rice seedlings were dipped in broth culture of...

Author(s): Md. Zahurul Islam, M. A. Sattar, M. Ashrafuzzaman, Halimi Mohd Saud and M. K. Uddin

January 2012

Histamine-producing bacteria isolated from frozen longtail tuna (Thunnus tonggoh)

In this investigation, a series of samples were carried out to isolate and identify histamine-producing bacteria and analyze the histamine content for evaluation of harvesting and post harvesting procedures. The target fish was longtail tuna (Thunnus tonggoh), which were collected from Oman Sea waters harvested by gillnet or purse seine methods. Bacteriological isolates and the amount of histamine were obtained from the...

Author(s): Koohdar Valiollah, Razavilar Vadood⊃, Kadivar Abolhassan and Shaghayegh Alireza

January 2012

Protein extraction methods for the two-dimensional gel electrophoresis analysis of the slow growing fungus Undifilum oxytropis

The fungus Undifilum oxytropis produces the toxin swainsonine and is symbiotic with locoweeds, which are toxic Oxytropis and Astragalus species. The genus Oxytropisincludes perennial legumes that are widespread in many rangeland regions around the world. Consumption of locoweeds causes significant livestock poisoning and severe economic losses. Information about swainsonine synthesis by the U....

Author(s): Haili Li, , Jianna Wang, Jianhua Wang, Guoxia Geng, Haocai Ju and Rebecca Creamer 

January 2012

Comparison of an automated system with four phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus

Correct and rapid detection of methicillin resistance in Staphylococcus aureus is very important for treatment of infected patients. Detection of the mecA gene or PBP2a by polymerase chain reaction (PCR) is considered the gold standard for determination of methicillin resistance in staphylococci. In most clinical laboratories, phenotypic methods are used for the detection of methicillin resistance in S....

Author(s): Meryem Iraz, Mehmet Sait Tekerekoglu, Baris Otlu and Selma Ay

January 2012

Frequency and complications of falciparum malaria among febrile staff members of UN deployed to Northern Sudan

Malaria is a mosquito-borne parasitic disease, common around the globe especially in poorest countries. It is inevitable and remediable, yet it is still killing many people every year, 90% of them are in Africa. Malaria is an important threat to tourists, soldiers and employees travelling or working in endemic areas. The present study was carried out among UN staff members working in regions of Damazin, AL Obayid, kosti...

Author(s): Abdul Majid, Sobia Kanwal, Iffat Naz, Shama Sehar and Tariq Mahmood

January 2012

Decolorization of textile dyes by Polyporus seudobetulinus and extracellular laccase

The objective of this study is to obtain new laccase and enzyme source with remarkable dye removal potential. Thirty isolates of white rot fungi were screened for extracellular laccase-production using 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) assay as indicator. Among these, Polyporus pseudobetulinus strain WR77 exhibits the highestlaccase activity and its suitable enzyme production medium...

Author(s): Pajareeya Songserm, Prakitsin Sihanonth, Polkit Sangvanich and Aphichart Karnchanatat

January 2012

Emergence of extended-spectrum β-lactamase producing Salmonella typhi in Pakistan

Enteric fever caused by Salmonella typhi, is an increasing health problem affecting the major population in tropical and subtropical regions of the world. Development of multi drug resistance in S. typhi strains has further increased the severity of the problem. In Pakistan and neighboring countries, more than 80% S. typhi strains have been reported as multi drug resistant. A total of 4200...

Author(s): Khalid Mahmood, Mateen Izhar, Nakhshab Choudhry, Ghulam Mujtaba and Naeem Rashid

January 2012

Is sustained release of vancomycin from fibrin glue effective to prevent methicillin-resistant Staphylococcus aureus graft infection?

Prosthetic vascular graft infection remains one of the most serious complications seen after vascular surgery. Recently, fibrin glue has gained attention as a possible means to deliver drug therapies. In this study, the efficacy of vancomycin incorporated fibrin glue for preventing methicillin-resistant Staphylococcus aureus (MRSA) infection of prosthetic grafts was investigated. The vascular grafts were...

Author(s): Nurkay Katrancioglu, Oguz Karahan, Ahmet Turhan Kilic, Ozgur Katrancioglu, Cem Celik, Mustafa Zahir Bakici, and Erhan Atahan

January 2012

Construction of a novel recombinant vector as Brucella melitensis vacB gene knockout candidate

Brucella melitensis is a Gram negative coccobacillus bacterium from the Brucellaceae family. Brucellosis is an infectious zoonotic disease affecting most species of domestic animals, but sheep and goats, particularly milking breeds, are the most susceptible. Cattle may occasionally be affected and the disease may appear in pigs. vacB gene is one important gene of B. melitensis that...

Author(s): Rahmatollah Yazdani, Abbas Doosti and Payam Ghasemi Dehkordi

January 2012

Evaluation of diagnostic performance of new antigen-based enzyme immune assay for diagnosis of Hepatitis C virus (HCV) infections

Laboratory diagnosis of Hepatitis C virus (HCV) is sometimes problematic. A novel immune enzyme assay (HCV-Core Antigen, Abbott Laboratories/Germany) has been recently licensed for detection of HCV antigen in human serum. In this study, we aimed to evaluate diagnostic performance of this new test in comparison with the HCV-RNA quantification and anti-HCV antibody (anti-HCV-Ab) analyses. A total of 152 serum samples of...

Author(s): Mehmet KöroÄŸlu, Sibel Ak, Muharrem Ak, Yusuf YakupoÄŸulları and Ali Özer

January 2012

Impacts of irrigation and non-irrigation conditions on some morphological traits of corn (Zea mays L.) cultivars

In order to study of impacts of irrigation and non-irrigation conditions on somemorphological traits of corn (Zea mays L.) cultivars, a factorial experiment based on randomized complete block design with three replications was arranged at Ardabil, Iran, in 2010. Factors included eight maize cultivars (BC 678, BC 418, SC 700, BC 582, SC 301, BC 652, BC 566 and SC...

Author(s): Mehdi Panahyan-e-Kivi, Shahzad Jamaati-e-Somarin and Roghayyeh Zabihi-e-Mahmoodabad

January 2012

Examination of red-leg disease and its pathogen, Rana temporaria chensinensis

Examination of pathogenic bacteria, isolated from Rana temporaria chensinensis, was conducted including morphological characteristics, physiological and biochemical characteristics, as well as the detection of the 16S rRNA gene sequence of the isolates. Phylogenetic tree based on the two sequences (HQ070610A-1, HQ070610B-1) and homologous sequences from GenBank was constructed by neighbor-joining method....

Author(s): GE Mu-xiang, ZHANG Yan-ying, FANG Hai, WANG Xiu-yun, JIN Xiao-min and CHEN Cui-Zhen

January 2012

Stimulative effect induced by low-concentration Cadmium in Lonicera japonica Thunb.

A hydroponics experiment was carried out to study the stimulative effect in Lonicera japonica Thunb. induced by cadmium (Cd). The results showed that the plants did not show any visual symptoms at low concentrations of Cd after 2 weeks exposure. Furthermore, roots elongation and contents of photosynthetic pigments displayed a biphasic curve with the increase of added Cd in nutrient medium, indicating...

Author(s): Lian Jia, Zhouli Liu, Wei Chen, Xingyuan He

January 2012

Evaluating antibacterial activity from essential oil of Artemisia fragrans Willd. in North-Western of Iran

The antibacterial effects of Artemisia fragrans essential oil were studied in this research. The composition of essential oil from aerial parts was analyzed by GC/MS and its antibacterial effects were determined by disk diffusion method. In this study the antibacterial effects of essential oil of A. fragrans on two types of Gram positive bacteriaStaphylococcus aureus, Strep. Enterococcus...

Author(s): Valizadeh Einollah, Jafari Behboud, Dolgari-Sharaf Jalil and Nari Bariji Vahid

January 2012

Biosynthesis of polyhydroxybutyrate (PHB) biopolymer by Bacillus megaterium SW1-2: Application of Box-Behnken design for optimization of process parameters

Bacillus megaterium SW1-2 producing polyhydroxalkanoate (PHA), previously isolated from activated sewage sludge was selected for the study of PHB biopolymer production. Preliminary experiment showed that the strain capable of producing 36% cell dry weight (CDW) of the polymer during growth on basal E2 medium supplemented with glucose, followed by 28, 15 and 8% CDW during growth on Na-acetate, lactose or...

Author(s): Mahmoud M. Berekaa and Ali M. Al Thawadi

January 2012

Isolation and characterization of a novel Micrococcus strain for bioremediation of strontium in radioactive residues

There has been increased interest in the isolation of bacteria with a potential role in bioremediation from extreme environments such as phosphate mines containing various metals and radionuclides. This paper describes the isolation and characterization of a novelMicrococcus species from a phosphate mining region in the South of Tunisia, designated as strain BRM7. Colonies of bacterial strain BRM7 produced on...

Author(s): Issam Ben Salem,#, Haïtham Sghaier, Houda Trifi, Sana Héni, Khaoula Khwaldia, Mouldi Saidi and Ahmed Landoulsi #First author contributed equally to this work.