African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 30 May, 2012; 6(20)

May 2012

Molecular cloning and phylogenetic analysis of the E gene of transmissible gastroenteritis virus (TGEV) isolated in China

Porcine transmissible gastroenteritis caused by transmissible gastroenteritis virus (TGEV)induces acute, high-contact intestinal infectious diseases in pigs. TGEV encodes four structural proteins including spike (S), membrane (M), envelope (E) and nucleoprotein (N), of these, the genes of S, M and N respectively had been deeply elucidated. However, the real function of E protein was still...

Author(s): D. L. Lan, J. P. Gu, R. Xing, C. L. Yuan, L. Cui, X. G. Hua and Z. B. Yang      

May 2012

Genotypic variability for tolerance to salinity and phosphorus deficiency among N2-dependent recombinant inbred lines of Common Bean (Phaseolus vulgaris)

Common bean (Phaseolus vulgaris L.) is often subject to various environmental constraints including soil salinity and phosphorus deficiency as major limitations for the yield of most grain legumes, especially when the plant growth depends upon N2 fixation. In order to assess the genetic variation for tolerance to moderate salinity and phosphorus deficiency and identify the related morphological, physiological...

Author(s): Boulbaba L’taief,,, Bouaziz Sifi, Mainassara Zaman-Allah, Ralf Horres, Carlos Molina, Steve Beebe, Peter Winter, Guenter Kahl, Jean-acques Drevonand Mokhtar Lachaâl        

May 2012

Optimization, production and purification of cellulase enzyme from marine Aspergillus flavus

Water samples were collected from five different places at Muthupettai mangrove along the east cost of Tamil Nadu, India. Five different Aspergillus species were isolated and identified by using lacto phenol cotton blue staining method. From this, Aspergillus flavuswas tested in Modified Park’s Agar for its cellulase enzyme production in different physico-chemical properties of substrates. The...

Author(s): Arun Sasi, Ravikumar M. and ManthiriKani S.

May 2012

Comparative analysis of endophytic mycobiota in different tissues of medicinal plants

Water samples were collected from five different places at Muthupettai mangrove along the east cost of Tamil Nadu, India. Five different Aspergillus species were isolated and identified by using lacto phenol cotton blue staining method. From this, Aspergillus flavuswas tested in Modified Park’s Agar for its cellulase enzyme production in different physico-chemical properties of substrates. The...

Author(s): Kilavan Packiam Kannan and Johnpaul Muthumary      

May 2012

The stability of micronutrients in fortified food stuffs after processing and storage: Iodine in salt and iron in wheat flour

Salt was fortified by potassium iodate in the level 66 mg/kg of salt with wet method of fortification. Then the iodized salt was stored using three packaging materials; low density polyethylene (LDPE), high density polyethylene (HDPE) and woven high density polyethylene (WHDPE) at two conditions: room temperature (20-25°C) and medium RH (50-60%) for about 6 months and accelerated temperature (40°C) and high...

Author(s): Abebe Ayelign, Kelbessa Urga and Negussie Retta    

May 2012

Temporal and spatial variations of phytoplankton in the Caspian Sea

Geographic information system (GIS) can be used to perform a number of fundamental spatial analysis operations. Its major advantage is that it allows the user to identify the spatial relationships between various map features. In this study, phytoplankton cell abundance, biomass and species composition were evaluated in the Iranian coasts of the Caspian Sea during 1996 to 1997 and 2005 to 2006. Totally 1562 water...

Author(s): Ali Ganjian Khenari, , , Maryam Ghasemnejad, Aabolghasem Roohi, Reza Pourgholam Wan Maznah Wan Omar, , Mashhor Mansor, Babak Mirbagheri and Alireza Ghaedi      

May 2012

NMR Shielding and thermodynamic density functional theory (DFT) studies of solvent effect on B10N11H7(Ala)2 Nano Cone

B3LYP/3-21G density functional theory (DFT) are carried out on the structure and stability of B10N11H7(Ala)2 in bulk and different solvent medium, using Gaussian 98 package of program. NMR parameters and thermodynamic properties were calculated to obtain stability and solvent effect.  It was found that computationally efficient solvent modeling is possible to find better results and also molecular structure of...

Author(s): M. Monajjemi, N. Hooshmand, H. Seyed Hosseini Ghaheh and F. Mollaamin      

May 2012

Effect of Ultraviolet-C (UV-C) irradiation on the virulence genes expression in Vibrio parahaemolyticus and Vibrio alginolyticus

In this study, Vibrio parahaemolyticus and Vibrio alginolyticus, marine foodborne pathogens, were treated with Ultraviolet-C (UV-C) irradiation (240 J.m-2) to evaluate alterations in their virulence genes expression levels. Firstly, we searched for the presence of eight Vibrio cholerae virulence genes, toxR, toxS, toxRS, ctxA, zot, ace, toxT, and virulence...

Author(s): Rihab Lagha#, Fethi Ben Abdallah, #, Ali Ellafi, Héla Kallel and Amina Bakhrouf        

May 2012

Characterization of Vibrio alginolyticus and Vibrio parahaemolyticus isolated from Penaeus monodon: Antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis

Vibrio alginolyticus and Vibrio parahaemolyticus are important pathogens which cause serious episode to shrimp. We characterize the suitability of randomly amplified polymorphic DNA (RAPD), and plasmid profiling for rapid molecular characterization of pathogenic bacteria for epidemiological and antibiogram study to find out the relationship between plasmids and bacterial sensitivity to...

Author(s): Nilima Priyadarshini Marhual, Basanta Kumar Das and Surya Kanta Samal        

May 2012

Seasonal examination of phytoplankton abundance and assemblages in Myall Lake and Bombah Broadwater, New South Wales, Australia

The Myall Lakes are a series of interconnected, shallow and poorly flushed coastal lakes located within the Myall Lakes National Park in NSW, Australia. The following field study aims to uncover patterns in temporal variability in water quality and phytoplankton abundance, diversity and assemblage structure, in the upper and lower water bodies of the Myall Lakes system. The result showed that the two lakes differ...

Author(s): Nita RUKMINASARI and Anna REDDEN      

May 2012

Xylanase production under solid state and submerged fermentation conditions by bacterial strains

Bacterial strains were isolated from soil samples collected at different parts of Western Ghats of India. The strains were screened for xylanase production using xylan-red agar medium. On the basis of clearing zones formed, twelve bacterial strains were selected and identified on the basis of 16S rRNA sequence. Solid state and submerged fermentations were done to identify strains that could produce maximum amount of...

Author(s): Rajashri D. Kamble and Anandrao R. Jadhav      

May 2012

Long-term outcomes of chronic hepatitis C patients treated with pegylated interferon 2a plus ribavirin in Iran

Combination of interferon alfa and ribavirin is the first therapeutic choice in chronic hepatitis C. The aim of this study is to assess long-term outcome of chronic hepatitis C patients treated with pegasys plus ribavirin. We evaluated a cohort of 49 chronic hepatitis C patients who were treated for 48 weeks with pegylated interferon alpha 2a and ribavirin and followed up till week 72 in order to evaluate sustained...

Author(s): Seyed Moayed Alavian, Majid Menati, Mahtab Shabani, Mostafa Shafiei and Mohammad Kolbadi Nejad      

May 2012

The effects of solid wood waste discharge on the physico-chemical and microbial characteristics of the Warri river

Water obtained from three locations from September to November, 2010 at the point of discharge of the sawmill wastes into the Warri river (Point 2) and at 100 m before (Point 1) and after (Point 3) the discharge were analyzed using standard methods to determine the effects of the wastes on the physico-chemical and microbial parameters of the River. While mean values for temperature, alkalinity, sulphate, carbonate,...

Author(s): Idise O.  E., Okoko F. J., Aire P., Ogor O. and Egbah A.        

May 2012

Evaluation of the reaction of durum wheat genotypes (Triticum durum Desf.) to drought conditions using various stress tolerance indices

In this study, 40 genotypes of durum wheat (Triticum durum) originating from Iran and Azerbaijan Republic were evaluated in both water-stressed and well-watered environments in 3 years 2008 to 2011 cropping years. In each environment, the genotypes were evaluated using a randomized complete block design with three replications. From the grain yield data, drought tolerance indices comprising of stability tolerance index...

Author(s): Majid Khayatnezhad      

May 2012

Seasonal influence on bioactivity of seaweeds against plant pathogenic bacteria Xanthomonas axonopodis pv. citri (Hasse) Vauterin et al.

In the present study, thin-layer chromatography (TLC) method was adopted for the fractionation of antibacterial substances from the crude extracts obtained in 1:1(v/v) chloroform : methanol of red seaweeds comprising Portieria hornemannii (Lyngbye) P. Silva, Gracilaria crassa (Harvey) J. Ag., brown Dictyota bartyrensiana Lamouroux,Hydroclathrus clathratus (C.Agardh)...

Author(s): Kulanthaiyesu Arunkumar and Sadaiyappa Ramasubbu Sivakumar      

May 2012

Pathogenicity of recent field isolate of Avian Adenovirus Serotype-IV of hydropericardium syndrome (Angara disease)

The pathogenicity of recent field isolate of Avian Adenovirus Serotype IV (AAS-IV) was determined during the present study. During the present investigations, Hydropericardium Hepatitis Syndrome (HHS) showed reduced pathogenicity as compared to the isolates studied on the inception of the syndrome in 1987-1988. Pathogenicity of the virus was determined through LD50 and challenge protection test. The LD50 titre...

Author(s): Shahzad Akbar Khan, Tarique hussain, Om Parkash and Sarfaraz Ali Fazlani      

May 2012

Antibiogram types of Klebsiella aerogenes isolated from urinary tract infection (UTI)

Thirty three (33) strains of Klebsiella aerogenes isolated from 197 in-and out-patients with symptoms of urinary tract infection (UTI) at University of Benin Teaching Hospital (UBTH), Benin City were used for this study. Twenty-one antibiotics were used for the antibiogram typing. Organisms were isolated from 65 positive samples, with the most prevalent beingStaphylococcus aureus (40.0%), others...

Author(s): Orhue P.O., Igumbor E.O., Enweani I.B., Esumeh F.I., Ohenhen R.E and Igbinosa E.O 

May 2012

Effect of row spacing and sowing date on yield and yield components of common bean (Phaseolus vulgaris L.)

Seed legumes are extremely important crop plants, which are widely cultivated in the Middle East. The study was conducted to determine the effect of different seeding times and seed rates on the growth, yield and quality of common bean (Phaseolus vulgaris L.). The experiment was laid out using RCBD under split plot arrangement with three replications. Three Row space as main plot R1 (50), R2 (40) and...

Author(s): Mahdi Babaeian, Mahdi. Javaheri and Ahmad. Asgharzade      

May 2012

Retracted: General steps in the production and establishment of transgenic chicken lines carrying a bacterial lacZ gene expressing β-galactosidase

Replication-defective retroviral vectors are efficient vehicles for the delivery of exogenous genes, and they may be used in the generation of transgenic animals. The replication-defective retroviral SNTZ vector carrying the lacZ gene with a nuclear localized signal was injected into the subgerminal cavity of freshly laid eggs. Subsequently, the eggs were allowed to hatch, and the chickens were screened for...

Author(s): Esmaeilzadeh Mahdi, Nejadali Abolfazl and Kazemzadeh Fariba      

May 2012

Biofilm production, adherence and hydrophobicity of starved Shigella in seawater

In this work, we incubated four Shigella strains for one month at room temperature and at 4°C. Their adhesion capacity to KB cells, biofilm formation on microplate and surface hydrophobicity of starved cells were investigated. Our results showed that the stressed cells exhibited a remarkable increase in their hydrophobicity from 19 to 34% and in their capacity to produce biofilm. Adherence assays to KB...

Author(s): Ali Ellafi, Rihab Lagha, Fethi Ben Abdallah and Amina Bakhrouf      

May 2012

Evaluation of in vitro antiviral activity of Chelidonium majus L. against herpes simplex virus type-1

Chelidonium majus Linnaeus (L) has been used in a variety of practical applications in medical sciences. Its antiviral activity against herpes simplex virus type-1 (HSV-1) was investigated in cell culture. C. majus L. extract was found to be non-toxic to Vero cells (African green monkey kidney cells) up to concentration 1000 mg/ml and inhibited the growth and development of HSV-1 in dose-dependent...

Author(s): Seyed Hamidreza Monavari, Mahmoud Shamsi Shahrabadi, Hossein Keyvani and Farah Bokharaei-Salim        

May 2012

Production and optimization of D-amino acid oxidase which is involved in the biosynthesis of β-lactam antibiotics

A total of sixty seven fungal isolates representing 40 fungal species related to 14 genera were screened for their abilities to produce endocellular D-amino acid oxidase enzyme. The most active fungal isolates were Fusarium heterosporum and Nectria haematococca, producing 210.41 and 207.94 units/ml, respectively. Maximum activity of D-amino acid oxidase produced by F....

Author(s): A. Saleem, A. M. Moharram and N. Fathy      

May 2012

Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1

Chitinase was isolated and purified from Gliocladium catenulatum through ammonium sulfate sedimentation, sephadex G-25 gel filtration, deionization, ultra filtration condensation, negative ion exchange separation, and non-denaturing gel electrophoresis. A 51 kDa chitinase was purified from the fungus through sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum temperature and pH for...

Author(s): Gui-Zhen Ma, Hui-Lan Gao, Yong-Hua Zhang, Shi-Dong Li, Bing-Yan Xie and Sheng-Jun Wu      

May 2012

Seroprevalence of leptospiral infection in horses, donkeys and mules in East Azerbaijan province

This study was conducted on 490 animals (390 horses, 80 donkeys and 20 mules) in East Azarbaijan province in Iran in order to verify seroprevalence of leptospiral infection in 2011. Sera were initially screened at dilution of 1:100 against 6 live serovars of Leptospira interrogans: Pomona, Canicola, Hardjo, Ballum, Icterohaemorrhagiae and Grippotyphosa using the microscopic agglutination test. The prevalence of...

Author(s): Hassanpour Ali and Safarmashaei Saeid      

May 2012

Chemical composition, in vitro antifungal and antioxidant activities of essential oil from Cotula coronopifolia L. growing in Tunisia

The chemical composition of Cotula coronopifolia L. essential oils obtained from the aerial parts (flowers, leaves, stems) and roots by hydro distillation was analyzed by gas chromatography/ mass spectrum (GC/MS). Fifty seven components were identified. The most important compound detected in leaves was Agarospirol (10.43%). Hexacosane was identified in flowers with an abundance percentage of (31.7%) and...

Author(s): Fethi Bel Hadj Kether, Mohamed Ali Mahjoub, Samia Ammar Mahjoub, Karima Belhaj Salah, Ahmed Noureddine Helal and Zine Mighri        

May 2012

Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey

The aim of this study is to investigate the antimicrobial effect of the lipopeptide biosurfactant produced by Bacillus licheniformis strain M104 grown on whey. The biosurfactant was investigated for potential antimicrobial activity by using disc-diffusion method against several Gram positive bacteria (Bacillus subtilis, Bacillus thuringiensis (2 strains), Bacillus cereus, Staphylococcus...

Author(s): Eman Zakaria Gomaa    

May 2012

Comparative inhibitory effect of xylitol and erythritol on the growth and biofilm formation of oral Streptococci

Our aims were to examine the effects of xylitol and a novel polyol sweetener, erythritol, on growth of oral Streptococci and compare their effects. The inhibitory effects of xylitol and erythritol on Streptococcus strains, as well as on streptococcal biofilm formation were examined. Streptococcus mutans, Streptococcus sobrinus, and Streptococcus sanguiniswere used as...

Author(s): Gholam Reza Ghezelbash, Iraj Nahvi and Mohammad Rabbani      

May 2012

Cyanobacterial abundance and diversity in coastal wetlands of Kanyakumari District, Tamil Nadu (India)

Coastal wetlands are physiologically unique from freshwater and marine water environments. They support the growth of both marine and freshwater organisms with affluent diversity. In this study, abundance and diversity of cyanobacteria from the coastal wetlands of Kanyakumari District (Tamil Nadu) in India was studied. Water samples were collected from ten different sampling sites during the month of January 2010 to...

Author(s): Sivakumar N., Viji V., Satheesh S., Varalakshmi P., Ashokkumar, B. and Pandi M.      

May 2012

Production, optimization and purification of lipase from Pseudomonas aeruginosa

Microbial lipases today occupy a place of prominence among biocatalysts owing to their ability to catalyze a wide variety of reactions. Out of 14 bacterial isolates obtained from wastewater sample. The isolates were grown on a selective medium agar that contained tween 80 or olive oil as the only source of carbon for detection of lipase activity 6 exhibited lipase activity. Pseudomonas aeruginosa (Ps5) was...

Author(s): Benattouche Zouaoui, Abbouni Bouziane and Bachir raho ghalem      

May 2012

Microorganisms associated with computer keyboards and mice in a university environment

A study was carried out to isolate and to identify microorganisms associated with computer keyboards and mice in computer centers and cyber café located in Kogi State University, Anyigba, Nigeria and its environs. Samples were collected from five different cyber café and computer centers. The samples were collected from three computer keyboards and mice in each cyber café and computer centers. The...

Author(s): S. C. Enemuor, T. A. Apeh and O. O. Oguntibeju