African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 30 November, 2011; 5(28)

November 2011

Epidemiology and antibiotic susceptibility of bacterial isolates from Northern Pakistan

  This study was conducted in Khyber Teaching Hospital, to evaluate antimicrobial susceptibility pattern among pathogens isolated in a tertiary care hospital. Specimens were collected in sterile containers and cultured for a period ranging from 24-48 h at 37°C aerobically, using appropriate bacteriological isolation media. Isolates were identified using Gram staining and biochemical reactions. Antibiotic...

Author(s): Jawad Ahmed, Abdul Hamid Jan, Ghareeb Nawaz and Mumraiz Khan

November 2011

Identification and partial characterization of antifungal and antibacterial activities of two Bacillus sp. strains isolated from salt soil in Tunisia

  Two Bacillus sp. strains (B29 and B27) isolated from soil in the South of Tunisia were tested for their abilities to produce antimicrobial compounds. Both strains showed antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi. The produced compounds were extracted by using four different solvents. The hexane extract allowed to obtain maximum of activity of the...

Author(s): Sihem Ben Maachia,, Rafik Errakhi, Florence Mathieu, Mohamed Chérif and  Ahmed Lebrihi

November 2011

Field assessment of calcium, copper and zinc ions on plant recovery and disease severity following infection of Huanglongbing (HLB) disease

  The effect of Ca, Cu and Zn ions on plant recovery and disease severity following infection of huanglongbing disease was carried out at a commercial citrus orchards belonging to Lembaga Pertubuhan Peladang (LPP) Terengganu, situated at Durian Mentangau, Dungun, Terengganu. Application of Zn or Cu ions in combination with Ca was able to delay disease incidence and severity of hunglongbing disease. These...

Author(s):   Khairulmazmi Ahmad, Kamaruzaman Sijam, Habibuddin Hashim, Zamri Rosli and Arifin Abdu

November 2011

The in vitro antioxidant properties of Bacillus simplex XJ-25 isolated from sand biological soil crusts

  XJ-25, a strain with strong antioxidant activity was isolated from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China. Strain XJ-25 is closely related to Bacillus simplex through the 16s rDNA sequencing combined with morphological, physiological and biochemical analysis. The medium for optimal antioxidant activity was NB with 1.5 g/Lglucose. Based on the kinetic assay, antioxidant...

Author(s): Z. R. Wang, J. P. Sheng, X. L. Tian, T. T. Wu, W. Z. Liu and L. Shen

November 2011

Characterisation of Bacillus strains from volcanic area Gunung Sibayak able to degrade 2,2-dichloropropionic acid

  Halogenated compounds are mainly found in agricultural area. The problem arose because these compounds are toxic to human and are persistent in environment. The aim of the present study was to isolate 2,2-dichloropropionic acid (2,2-DCP) degrading bacteria that can grow at higher temperature rather than at normal 25 to 30°C temperature. A soil bacterium was isolated from the surrounding volcanic area at...

Author(s): Damia Diyana Roslan, Ronnie G. Gicana, Robert J. Lamis and Fahrul Huyop

November 2011

The anti-bacterial effects of Zataria multiflora extract on common pathogenic Gram positive cocci, pathogenic Gram negative bacilli and non -pathogenic bacteria

  Due to increased bacterial resistance to common antibiotics, tendency towards using herbal extracts like Zataria multiflora from Lamiaceae family is increasing. In this study, antibacterial effects of Z. multiflora on several common pathogenic and non pathogenic bacteria were evaluated. Several clinical Gram positive cocci and Gram negative bacilli isolated from patients and healthy humans...

Author(s): Motevasel M., Zomorodian K., Ashraf Mansouri M. A., Farshad S. H., Haghighhat A. R., Hadaegh M. G. and Takhshid M. A.

November 2011

Analysis of the polymorphism of Staphylococcus strains isolated from a hospital environment

  Nosocomial infections, despite the introduction of the numerous control systems, still constitute a considerable problem in hospitals. They are of great socio-economic importance due to the prolonged stay of patients in hospitals and high treatment costs. They also contribute to an increase in the death rate. For many years staphylococci have remained at the top of the list of microorganisms that cause...

Author(s): Robert D. Wojtyczka, Daniel Krakowian, Lukasz Marek, Dominik Skiba, Adam Kudelski, Krzysztof Jasik and Jerzy Pacha

November 2011

Effect of arbuscular mycorrhizal (AM) inoculation on growth of Chili plant in organic manure amended soil

  Arbuscular mycorrhizal (AM) fungi are well known for their plant growth promoting efficiency and providing bio-protection against soil–borne pathogens (bacterial, fungal and parasitic nematodes). The efficiency of AM fungi as bio-control agents in commercial organic agriculture is gaining significance in recent decades. However, little information is available regarding beneficial role of AM symbiosis...

Author(s): Oyi Dai, R. K. Singh and Gibji Nimasow

November 2011

Biodegradation of low density polyethylene (LDPE) by fungi isolated from marine water– a SEM analysis

  Fungi, isolated from sea water, were subjected to growth in a medium containing low density polyethylene (LDPE) as the sole carbon source with and without yeast extract. Increasing fresh weight of the fungi in the medium supplemented with LDPE after regular time intervals gave the evidence that the fungi were utilizing LDPE as the carbon source. Further confirmation of LDPE utilization was carried out by the...

Author(s): R. Pramila and K. Vijaya Ramesh

November 2011

Isolation and molecular detection of Plesiomonas shigelloides containing tetA gene from Asian arowana (Scleropages formosus) in a Korean aquarium

  An Asian arowana (dragonfish; Scleropages formosus) that had been reared for public exhibition in a private commercial aquarium in Seoul Korea was recently found dead. A bacterial pathogen was cultured from the fish’s kidney, identified, and confirmed asPlesiomonas shigelloides, using a Vitek System®2, PCR and 16S rRNA gene sequencing. The isolated strain proved resistant to commercial...

Author(s): Jin Woo Jun, Ji Hyung Kim, Casiano H. Choresca Jr., Sang Phil Shin, Jee Eun Han, Dal Sang Jeong and Se Chang Park

November 2011

Poly-3-hydroxybutyrate production from methanol by Methylosinus trichosporium IMV3011 in the non-sterilized fed-batch fermentation

  The methanotrophic bacteria have promising applications in the production of a biopolymer, poly-3-hydroxybutyrate (PHB). The fed-batch fermentation strategies were studied for PHB accumulation by methanol-grown Methylosinus trichosporium IMV3011 under the non-sterilized conditions. The biomass of 2.91 g dry wt/L and PHB accumulation of 47.6% (w/w) were reached in the fed-batch fermentation. In order...

Author(s): Hao Song, Jiaying Xin, Yingxin Zhang, Weibao Kong, and Chungu Xia

November 2011

Improve lipid production by pH shifted-strategy in batch culture of Chlorella protothecoides

  Effects of culture broth pH values on cells growth and lipid production were investigated in heterotrophic cultivation of Chlorella protothecoides. Based on the phenomenon that lower pH value favors cells growth but retards lipid synthesis and vice versa for higher pH value, a novel pH-shift strategy, optimized via simulating Gauss function, was developed. By controlling pH at 5.0 for first 93 h and...

Author(s): Guobin Liang, Yiwei Mo, Jianghong Tang and Quanfan Zhou

November 2011

Antibiotic activity of bacterial isolates associated with entomopathogenic nematodes

  In the present study, we isolated 23 strains of symbiotic bacteria from 23 entomopathogenic nematodes strains gathered in different vegetation from different regions of China. The antibiotic activities of all these bacteria strains isolated were evaluated in the laboratory. All the bacteria effectively inhibited seven kinds of plant pathogenic fungi (Bipolaris sorokinianum, Fusarium...

Author(s):   Huan Wang, Yan-Qun Liu, Hui Dong, Li Qin, Bin Cong and Tian-Lai Li  

November 2011

Production and application of glucose-fructose oxidoreductase for conversion of pineapple juice sugars

  This work is concerned with the production of glucose-fructose oxidoreductase (GFOR) enzyme for converting pineapple juice sugars into dietetic derivatives. GFOR was produced from Zymomonas mobilis by sub-merge fermentation of 10 L. During fermentation, the highest biomass formation was observed at 24 h and the highest enzyme activity per g biomass was observed at 27 h of...

Author(s): M. G. Aziz, Y. A. Yusof and K. D. Kulbe

November 2011

Combinations of spectrofluorometry and polymerase chain reaction for rapid detection of medium-chain-length polyhydroxyalkanoate-accumulating (Mcl-PHA) bacteria

  Medium-chain-length polyhydroxyalkanoates (Mcl-PHAs) share physical and material properties that are considered as an alternative to nonbiodegradable plastics. In this report, a new method was developed for detection of Mcl-PHA-producing bacteria. The procedure was composed of, first staining with Nile red and screen under fluorescence microscope at a wavelength of 565 nm, then further confirmation by...

Author(s): Zhao Chao, and Ruan Lingwei

November 2011

Application of lipase treatment on carotenoids extraction from Chili (Capsicum annuum L.)

  Five strains of lipase-producing bacteria were selected and determined for enzyme activity. As well as, the potential of enzymatic extraction on carotenoids from dried chili was also investigated. Among five strains, Bacillus subtilis yielded the highest lipase activity (25.5±0.2 U/ml) within 60 days of cultivation followed by Candida tropicalis(19.1±0.3 U/ml), Candida...

Author(s): Kanokphorn Sangkharak, Piyaporn Pasitkul, Nisa Paiyit and Pongnarin  Aonchart  

November 2011

Prevalence of Neospora caninum antibodies in Shepherd dogs in Sarab district, East Azerbaijan Province, Iran

  Neospora caninum is a heteroxenous cyst-forming apicomplexan intracellular protozoan which is considered as a major cause of infectious bovine abortion worldwide. The aim of the present study was to compile the initial epidemiological data on the prevalence of N. caninum antibodies in Sarab area. 384 blood samples were collected. Serum samples were tested for the presence of N....

Author(s): Khanmohammadi M. and Fallah E.

November 2011

Evaluation of Candida isolation from vaginal mucosa of mothers and oral mucosa of neonates on the basis of delivery type

  Candida spp. is one of the most important fungal pathogens in the intensive neonatal care units associated with increased health care costs. This study was performed on 164 pregnant women (82 vaginal-routes and 82 cesarean-routes) and their infants. The vaginal samples were obtained using swabs from mothers prior to delivery. Swabs were taken from oral mucosa of all infants immediately after birth as...

Author(s): Jamileh bigom Taheri ,Hamed Mortazavi ,Saeedeh Mohammadi,  Sedigheh Bakhtiari , Fatemeh Namazi,Naser Valaei,Mahin Bakhshi, Maryam Baharvand,Nika Bahemmat

November 2011

The vascular endothelial growth factor interacts with Mycobacterium tuberculosis ESAT-6

  Mycobacterium tuberculosis continues to be a leading cause of human deaths due to an infectious agent. The ESAT-6 protein of Mycobacterium tuberculosis (M.tb) is an important structural and functional protein, which has been known to be involved in the virulence, pathogenesis as well as proliferation of the pathogen, however, little is still known about the host factors that interact with...

Author(s):   Yanfen Wang, Ying Xiao, Yanhui Li, Yanhua Feng, Chunping Li and Haichun Ma  

November 2011

Purification and biochemical characterization of alkaline protease from an Egyptian biopesticide-producing Bacillus sphaericus strain

  The objective of the current study was to purify and partially characterize an alkaline protease (AP) from a newly isolated Egyptian Bacillus sphaericus strain. The enzyme was subjected to a 3-step purification scheme involving i) ammonium sulfate [(NH4)2SO4] fractionation, ii) acetone precipitation and iii) Sephadex G-200 gel permeation chromatography. Fractions precipitated with 30 to 60%...

Author(s): Mourad A. M. Aboul-Soud, Abd El-Moneim MR Afify, Mohammed S. Foda, Tarik Kahil, Amira R. Asar, Gaber E. El-Desoky, Abdulaziz M. Al-Othman, Zeid A. Al-Othman and John P. Giesy,,

November 2011

The role of trace elements in wagner classified diabetic patients

  Wagner's classification is the most widely used grading system in diabetic foot infections. The aim of this study was to evaluate the role of the trace elements in diabetic foot infected patients graded with Wagner's classification. This prospective study was carried out on 50 diabetic patients with variable severity of foot infections and 50 age and sex matched healthy subjects in Dicle University,...

Author(s): Fatma Bozkurt, Serda GULSUN, Cemal USTUN, M. Faruk Geyik, Ibrahim TEĞİN and Ömer SATICI

November 2011

Normal bacterial flora from idmi (Gazella gazella) and reem gazelles (Gazella subgutturosa marica) in Saudi Arabia

  Nasal, buccal and rectal swabs were collected from 24 adults and 25 newborn clinically healthy reem (Gazella subgutturosa marica) and idmi (Gazella gazella) gazelles from the breeding pens of the King Khalid Wildlife Research Center (KKWRC), Riyadh, Saudi Arabia. Swabs were cultured on bacteriological media and organisms isolated were identified using the appropriate analytical profile index (API)...

Author(s): Mohammed S. Al Saggaf, Sawsan A. Omer, Abdulla S. Khaliel, Abdulaziz N. Alagaili and Osama B. Mohammed

November 2011

Application of PCR for diagnosis of contagious Agalactia in Khuzestan Province-Iran

  DNA amplification technique which is called PCR is a precise and specific test method and nowadays is also used for identification of Mycoplasma, the causative agent of contagious agalactia in sheep and goats. It also avoids the antigenic cross reaction and variability that hamper serological methods. Several sets of primers are available for different members of Mycoplasma such...

Author(s):   Pooladgar A. R., Rahimilarki E, Ghaem Maghami S., Hossieni S. M. H. and Ghaleh Golab B.

November 2011

Characterization of a new species of neisseria isolated from the liver of the gaoyou sheldrake

  A strain of Gram-negative diplococci was isolated from the liver of the Gaoyou Sheldrake. The 16S rRNA gene sequences of this organism was most similar to Bacterium ‘New Zealand A’ (GenBank accession no. AY721627), with 97% similarity to Neisseria animaloris in the Neisseria genus. It showed a diplococci morphology by electron microscopy. The biochemical reactions of this strain...

Author(s): Wang Yanhong, Wang xiaoquan, Liu wenbo, Yang yuefei, Zhu jie, Shao hui-jun, Zhang bin-bin, Han tao, and Liu Xiufan

November 2011

Mycelial growth requirements of Lactarius pyrogalus and Lactarius controversus

The objective of this study was to determine the requirements including culture medium, temperature, pH, carbon and nitrogen sources for the best mycelium growth of Lactarius pyrogalus and Lactarius controversus. Different inoculum media containing peat and peat : vermiculite mixtures (1:4, 1:6, 1:8 and 1:10 v:v) were also investigated for vegetative inoculum production. In terms of mycelium growth rate...

Author(s): Beyhan Kibar and Aysun PekÅŸen

November 2011

Biological activity of the n-butanolic extract of Stachys pilifera

  The n-butanolic extract of Stachys pilifera growing in Iran have been screened for antimicrobial, antioxidant and antitumor activity. The antimicrobial capacity of this extract was evaluated against Gram-positive and Gram-negative bacteria, and fungi. The extract was showed mildly significant activity against these microorganisms. The antioxidant activities were evaluated using...

Author(s): Mohammad Hossein Farjam, Mojtaba Khalili, Abdolhossein Rustayian, Katayoun Javidnia and Sedigheh Izadi

November 2011

A PCR screen for malaria carrier infections using human saliva samples

  With endemic countries now aiming for elimination, the detection of malaria infections, with or without symptoms, is increasingly important for monitoring and evaluation programmes. Current malaria screening methods necessitate blood withdrawal. This invasive approach is constrained, especially for identifying the asymptomatic carrier reservoir, since segments of communities with blood taboos avoid...

Author(s): Ofentse J. Pooe, Addmore Shonhai and Sungano Mharakurwa,  

November 2011

Changes on proline, phenolics compounds and antioxidants status in Euglena gracilis exposed to copper

  The effects of copper toxicity on the content of bioactive compounds in Euglena graciliswere investigated using two CuSO4 concentrations (0.4 and 0.8 mM) added in medium culture. Proline, phenolic compounds and antioxidant activity were measured after 72 h of copper stress. Results obtained in this study show that increasing levels of copper (0.4 and 0.8 mM) resulted in a general...

Author(s):   David Cervantes-García, Daniel González-Mendoza, Onecimo Grimaldo-Juárez and Silvia Aviles-Marin

November 2011

Detection of the antibiotic resistance genes in Staphylococcus aureus isolated from human infections and bovine mastitis

  The present study was carried out in an attempt to detect the distribution of antibiotic-resistant genes of Staphylococcus aureus isolates from human infections and bovine mastitis. mecA, msrA, msrB, aacA-D, tetK and tetM genes were selected in order to detect the distribution of antibiotic-resistant genes by multiplex PCR technique. According to the biochemical analysis and...

Author(s): Malihe Heidari, Hassan Momtaz and Mahboobeh Madani

November 2011

Scrotal, testicular and semen characteristics of the mountain gazelle (Gazella gazella) males

  This study aimed to evaluate seasonal differences in the characteristics of various male reproductive organs and in semen quality. Ten adult males of mountain gazelles (Gazella gazelle) were used in this study during summer and winter. The results showed significant differences in July to September versus October to December for volume (P≤0.05), motility and viability (P≤0.01), progressive...

Author(s):   Al-Eissa Mohammed Saad, Alhamidi A. R., Mohamed Abd El Kader Sandouka, Ibraheem A. S., Hamad Al-Yahya, AL-Farraj S., Saad Alkahtani and Al-Dahmash B.