February 2011
Eco-friendly role of biodegradation against agricultural pesticides hazards
This review aims to elaborate the potential applications of various biological agents in decontamination of agricultural soils, which have been polluted with continuous and higher doses of pesticides through process of biodegradation. Biodegradation is an eco friendly, cost effective, highly efficient approach and can be considered as a superior alternative to physical and chemical methods which are not only...
February 2011
Antimicrobial susceptibility of Escherichia coli and other coliforms isolated from urine of asymptomatic students in Bayelsa State, Nigeria
This study investigated the isolation rate and antimicrobial susceptibility of Escherichia coliand other coliforms from asymptomatic male and female students of Niger Delta University in Bayelsa State, Nigeria. E. coli and other coliforms from midstream clean-catch urine samples of asymptomatic male and female students were isolated and tested for their susceptibility to commonly used...
February 2011
Identification and quantitation of conjunctival aerobic bacterial flora from healthy residents at different ages in Southwest China
To identify and quantitate conjunctival aerobic bacterial flora from healthy residents of three age groups in Chongqing city, Southwest China. Samples taken with moist swabs from the lower fornix of bilateral eyes of 33 children (9.85 ± 0.57 years old), 31 youths (21.23 ± 0.88 years old), and 30 elders (72.97 ± 4.94 years old) respectively were tested for bacterial cultures. Positive...
February 2011
Microbial quality, diversity and antibiotic susceptibility profiles of bacterial isolates from borehole water used by schools in Greater Giyani Municipality, Mopani District, South Africa
In the present study, the microbial quality of several boreholes, used by rural schools in Greater Giyani Municipality, was assessed over a six months period to determine their safety for human consumption and to highlight the potential occurrence of water-borne diseases. The microbiological quality of the water sources was performed using the membrane filtration technique, while standard culture methods were...
February 2011
A novel vector for expression cloning of large numbers of polymerase chain reaction products in Pichia pastoris
We described construction of a novel vector, pAOX-HT, for direct cloning of polymerase chain reaction (PCR) amplified fragments and expression in Pichia pastoris. The pAOX-HT serves both as a T-vector and expression vector and can be generated from a parent plasmid by digestion with restriction enzyme XcmI. To minimize the non-recombinant background, the parent vector pAOX-HT-BSK was designed to...
February 2011
Identification of appropriate sample and culture method for isolation of new thermophilic bacteria from hot spring
The purpose of this study was to identify appropriate samples and culture techniques on the isolation of thermophiles from local hot spring. Samples obtained from hot spring were used to assess the effects of sample type (water, biomat and sediment) and culture technique (on site or in laboratory after shipping in thermo flask) on thermopiles isolation rate. Overall, isolation rates were higher from...
February 2011
Species composition and molecular analysis of symbiotic fungi in roots of Changnienia amoena (Orchidaceae)
The diversity and population structure of symbiotic fungi in Changnienia amoena(Orchidaceae) were carried out. A total of 67 isolates were recovered from root samples, all isolates were identified based on morphological criteria and rDNA ITS phylogeny.Overall, 18 taxa were detected. Ascomycetes were the major fungal group, the remaining three isolates were genetically close...
February 2011
The effect of soil water potential on survival of fecal coliforms in soil treated with organic wastes under laboratory conditions
Pollution caused by animal wastes has become a great problem in many countries. The objective of this study was to test the effects of water potential on survival of fecal coliforms in a soil treated with 3 manures. A semiarid soil was treated with raw cow and poultry manures (CM and PM) and sewage sludge (SS) at a rate of 20 g kg-1 (dry weight basis). Three water potentials established for soil...
February 2011
Fibrolytic potential of spent compost of the mushroom Agaricus bisporus to degrade forages for ruminants
To evaluate the fibrolytic potential to degrade forages, extracts from spent compost ofAgaricus bisporus (SCAB) were evaluated in vitro. Firstly, SCAB was cultivated for 50, 60 and 90 days (culture stage) and enzyme activity was evaluated. The highest cellulase activity was at 60 days; xylanase activity was not affected by culture stage and laccase activity increased as culture stage increased....
February 2011
Quantum mechanic studies on dipalmitoylphosphatidylcholine and dimyristoylphosphatidylcoline as nanoscales of membrane
An understanding of static properties of membrane is an essential prelude to the study of movement of molecules within the membrane. In this investigation two molecules have been theoretically investigated through the quantum mechanical calculations. According to the obtained results of the structural optimization of the isolated DPPC (dipalmitoylphosphatidylcholine) and DMPC (dimyristoylphosphatidylcoline)...
January 2011
The effects of nutritional restriction on neutral lipid accumulation in Chlamydomonas and Chlorella
Under nutrient starvation conditions, many microalgae are known to accumulate triacylglycerols (TAG) that can be used for biodiesel production. However, few studies have been performed to analyze the effect of deficiency in nutrient elements such as sulfur, phosphorus, potassium, iron, magnesium and calcium on oil production, particularly in Chlamydomonas and Chlorella. In this study, we...
February 2011
A novel male-specific DNA sequence for river buffaloes
In this paper, we report identification of a novel male-specific DNA marker in Iranian river buffalo presenting the possibility of river buffalo gender determination using Random amplified polymorphic DNA-PCR (RAPD-PCR). Twenty eight random primers used for RAPD fingerprinting in order to isolation and characterization of microsatellite sequences using PIMA (PCR-based isolation of microsatellite array). All...
February 2011
Distribution, antibiogram and multidrug resistance in Enterobacteriaceae from commercial poultry feeds in Nigeria
The increasing incidence of enterobacteriosis in poultry birds in Nigeria lately necessitated the study which aimed at enumerating the Enterobacteriaceae in 58 commercially available poultry feeds, studying their antibiotic susceptibility pattern, confirming plasmid as the determinants of resistance and tracing the relationship between multidrug resistant (MDR) isolates in the feed and those in morbid birds....
February 2011
In vitro creation of artificial nitrogen fixing Cyanobacterium (Nostoc muscorum) association with wheat
Using sonication technique and addition of 2,4 dichlorophenoxyacetic acid to wheat roots, increasing colonization of Nostoc muscorum on roots and enhancement of nitrogen fixation as novel associations between nitrogen fixing Nostoc muscorum and nonlegume wheat, had been occurred. Sonication of roots (10 and 20 s) enhanced abundance of N. muscorum within / on the root...
February 2011
In vitro effect of tigecycline against Mycobacterium tuberculosis and a review of the available drugs for tuberculosis
The study aimed to investigate the effectiveness of a novel antibiotic drug tigecycline on clinical isolates of Mycobacterium tuberculosis and, reviewing defined anti-tuberculosis effects of available agents. Minimal inhibitory concentration (MIC) of tigecycline for 50 M. tuberculosisincluding multidrug-resistant (MDR) clinical isolates (20 MDR isolates) was determined by broth...
February 2011
Degradation of purine ribonucleosides by extracts of Penicillium viridicatum
Cell-free extracts of nitrate-grown Penicillium viridicatum could catalyze the hydrolytic cleavage of N-glycosidic bond of adenosine, guanosine and inosine to the corresponding base and ribose by a ribonucleoside hydrolase; however, there is no evidence for the degradation of these compounds through phosphorylation. The rate of hydrolysis of the three ribonucleosides was in the order inosine>...
February 2011
In vitro evaluation of anti-staphylococcal activity of Ganoderma lucidum, Ganoderma praelongum and Ganoderma resinaceum from Pune, India
Antimicrobial activity of Ganoderma praelongum, Ganoderma resinaceum andGanoderma lucidum were evaluated against thirty strains of clinical isolates of methicillin resistant and methicillin sensitive Staphylococcus aureus (MSSA) by the well agar diffusion and microtiter plate dilution. Chloroform, ethyl acetate, methanol, and water were used as extractive solvents. Standard antibiotic...
February 2011
Synthesis, characterization and biological activity of vic-dioxime derivatives containing benzaldehydehydrazone groups and their metal complexes
In this study, three new vic-dioxime derivatives containing benzaldehydehydrazone groups (L1H2: 4-methoxybenzaldehydehydrazone glyoxime, L2H2: 4-methylbenzaldehydehydrazone glyoxime and L3H2: 3-methylbenzaldehydehydrazone glyoxime) and their Ni(II), Cu(II) and Co(II) complexes are used. The biological activity of these aromatic hydrazone-oxime derivatives has been determined in both prokaryotic and...
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