African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 4 July, 2011; 5(13)

July 2011

Arbuscular mycorrhizal fungi and heavy metal contaminated soils

Arbuscular mycorrhizal fungi (AMF) associations are integral, functioning parts of plant roots and are widely recognized as enhancing plant growth on severely disturbed sites, including those contaminated with heavy metals (HMs). They are reported to be present on the roots of plants growing on HM-contaminated soils and play an important role in metal tolerance and accumulation. Isolation of the indigenous and...

Author(s): Akbar Karimi, Habib Khodaverdiloo, Mozhgan Sepehri and Mirhassan Rasouli Sadaghiani

July 2011

Fungal sinusitis in immunocompromised hosts

The term “immunocompromised host” is generally applied to a variety of patients with various immune defects. Invasive rhinosinusitis is defined by the presence of local inflammation, with vascular and osseous necrosis with extensive soft tissue extension, and occurs almost exclusively in immunocompromised patients. Fungi have been increasingly recognized as important pathogens in severe acute and...

Author(s): Tayfun Apuhan, Abdulkadir Küçükbayrak and Ismail Hakyemez

July 2011

Investigation of lactobacilli from mother’s breast milk who were placed on probiotic diet

Human milk contains a group of bacteria with potential to be used as probiotics but the origin of these bacteria is debated. This study was aimed to identify and isolate lactobacilli in breast milk and investigate the role of consuming probiotic supplements on the lactobacilli microflora of breast milk. Ten out of twenty mothers were placed on the probiotic diet and the others were considered as controls. The...

Author(s): Leila Roozbeh Nasiraii, Farideh Tabatabaie, Behzad Alaeddini, Reihaneh Noorbakhsh, Reza Majidzadeh Heravi and Shahnas Afsharian

July 2011

Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) fingerprinting reveals intra-serotype variations among circulating Listeria monocytogenes strains

Forty-five presumptive Listeria monocytogenes isolates were confirmed by multiplexpolymerase chain reaction (PCR) and characterized for antimicrobial susceptibility and tolerance to commonly used disinfectants. Isolates were also serotyped by PCR and characterized by enterobacterial repetitive intergenic consensus ERIC-PCR fingerprinting. All of the isolates showed PCR products of 938 bp (genus) and...

Author(s): Zulema Ruiz-Bolivar, Ana K. Carrascal-Camacho, Magda C. Neuque-Rico, Carolina Gutiérrez-Triviño, María X. Rodríguez-Bocanegra, Raúl A. Poutou-Piñales and  Salim Mattar

July 2011

Identification and partial characterization of antifungal and antibacterial activities of two Bacillus sp. strains isolated from salt soil in Tunisia

Two Bacillus sp. strains (B29 and B27) isolated from soil in the South of Tunisia were tested for their abilities to produce antimicrobial compounds. Both strains showed antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi. The produced compounds were extracted by using four different solvents. The hexane solvent allowed to obtain maximum of activity of the strain B29. The...

Author(s): Ben Maachia Sihem,, Errakhi Rafik, Mathieu Florence, Chérif Mohamed, Lebrihi Ahmed

July 2011

Microbiological changes in Lighvan cheese throughout its manufacture and ripening

The aim of this research was to monitor the microbiological changes throughout the manufacture and ripening of Lighvan cheese. In summer 2009, ten independent batches of cheese were manufactured by experienced workers without any intervention by the researcher. Duplicate samples of raw milk, coagulum and 0, 15, 30, 60 and 90 days old cheese were analyzed. The main groups, such as mesophilic Lactococci,...

Author(s): Mirzaei H.

July 2011

Nasal carriage and methicillin resistance of Staphylococcus aureus in patients and hospital staff in a tertiary referral center setting

The prevalence of nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) were studied from June 2006 – 2007 among in-patients and hospital staff in a tertiary referral center setting in Ankara, Turkey. Methicillin resistance was evaluated by Kirby-Bauer disc diffusion method. Of 438 people, 106 (24.2%) were nasal carriers of S. aureus. The prevalence of...

Author(s): S. Citak, F. N. Bayazit and F. Aksoy

July 2011

Screening and production of antibacterial compound from Trichoderma spp. against human-pathogenic bacteria

This study focus on the production of antibacterial compound from Trichoderma spp. Screening of antibacterial activities in some Trichoderma spp. was investigated using CYS80 medium. Trichoderma reesei and Trichoderma viride were highly effective toward human-pathogenic bacteria tested. T. viride and T. reesei were separately applied on Sephacryl...

Author(s): Rashad M. Saleh, Saleh A. Kabli, Saleh M. Al-Garni and Saleh A. Mohamed,

July 2011

Street foods: Handling, hygiene and client expectations in a World Heritage Site Town, Cape Coast, Ghana

Handling, vending and hygienic quality of street foods available to local residents, internal and foreign tourists to Cape Coast, the most important tourism hub in Ghana, were investigated. Questionnaires assessed stakeholder commitment to and expectations of food hygiene. Laboratory analysis evaluated microbial contamination levels of the street foods. Patronised by both local residents and tourists, foreign tourists...

Author(s): A. Annan-Prah, D. H. A. K. Amewowor, J. Osei-Kofi, S. E. Amoono, S. Y. Akorli,  E. Saka and H. A. Ndadi

July 2011

Isolation and identification of N-acylhomoserin lactone degrading bacteria from potato rhizosphere

In many Gram-negative bacteria, including a number of pathogens such asPectobacterium carotovorum, virulence factor production and many functions are linked to the quorum sensing (QS) systems that use diffusible N-acyl homoserine lactones (AHLs) as intercellular messenger molecules. A number of organisms also contain genes that hydrolyze AHLs into inactive products, thereby blocking the quorum-sensing systems. In...

Author(s): Esmaeil Mahmoudi, Nader Hasanzadeh, Badraldin Ebrahim Sayed Tabatabaei and Vittorio Venturi

July 2011

Prokaryotic expression and purification of grass carp reovirus capsid protein VP7 and its vaccine potential

The 11 dsRNA fragmental genome of grass carp reovirus (GCRV) is enclosed in five inner core proteins and two outer capsid proteins. The Glutathione S-transferase (GST) fusion protein expression vector pGEX-4T-3 was employed to clone and expression of GCRV outer capsid gene vp7, which was amplified by reverse transcription-polymerase chain reaction (RT - PCR) from infected Grass carp. The recombinant GST-fusion...

Author(s): Yongxing He, Qian Yang, Hongxu Xu, Hao Wu, Fangyuan Wu and Liqun Lu

July 2011

Statistical optimization of cultural conditions for chitinase production from shrimp shellfish waste by Alternaria alternata

Alternaria alternata, a local isolate from fishery polluted soil, was used successfully for the biodegradation of shrimp shellfish waste in favor of the production of highly active chitinase enzyme. Chitinase production was noticeably influenced by the culture medium and the highest enzyme production was attained through the log growth phase (96 h). Pronounced increase in chitinase production was concomitant with...

Author(s): Khaled M. Ghanem, Fahad A. Al-Fassi and Reem M. Farsi

July 2011

Management of ufra disease of rice caused by Ditylenchus angustus with nematicides and resistance

The effects of nematicide application timing and type on the management of ufra disease of rice caused by Ditylenchus angustus were investigated in the rain-fed and irrigated rice ecosystems. A broad range of rice (Oryza sativa L.) genotypes were also screened for resistance to D. angustus. Ufra infestation was significantly reduced when 1 kg ai/ha furadan 5G was applied up to 20 d before...

Author(s): M. A. Latif, , M. W. Ullah, M. Y. Rafii and M. I. Tajul

July 2011

Detection of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) methods from Iranian goats

Mycoplasma agalactiae is the etiological agent of contagious agalactia that is a serious disease affecting sheep and goats. It is characterized by mastitis and subsequent failure of milk production, arthritis and keratoconjunctivitis. The aim of the study was to detection of M. agalactiae by culture and polymerase chain reaction (PCR) methods from infected Iranian goats. For detection, a total...

Author(s): Babak Kheirkhah, Seyed Ali Pourbakhsh, Mohammad-gholi Nadalian, Mansour Banani and Abbas Ashtari

July 2011

Biodegradation of swainsonine by five types of plasmid-transformants from genomic library of Arthrobacter sp. HW08

The aim of this study is to analyze the ability of swainsonine (SW) biodegradation by five types of transformants from the genomic library of Arthrobacter sp. HW08, explore preliminarily metabolites and deduce the metabolic pathway. Using 1000 mg·l-1 SW as the sole carbon source, MSM containing five transformants at a proportion for 1:1(V:V) was incubated at 30°C, 180 r·min-1. The...

Author(s): Yanchun Hu, Yan Wang, Jianna Wang, Guodong Yang, Haili Li, Guoxia Gengand Jianhua Wang

July 2011

Seroprevalence of caprine brucellosis and associated risk factors in South Omo Zone of Southern Ethiopia

A cross - sectional study was conducted in Hammer and Dasenech Districts of South Omo Zone to determine seroprevalence of caprine brucellosis and its potential risk factors. Simple random sampling method was used to select 384 adult goats with no previous history of vaccination against brucellosis in the two districts. Modified Rose Bengal Plate Test (mRBPT) and complement fixation test (CFT) were used as screening...

Author(s): Tigist Ashagrie, Yosefe Deneke and Tadele Tolosa

July 2011

Screening and evaluation of antiphytopathogenic activity of endophytic fungi from live foliages of Ginkgo biobla L.

Twenty-four strains of endophytic fungi were isolated from 50 fresh leaves samples ofGinkgo biobla L collected using the cultivation-dependent method from the campus of Anhui agricultural university in Hefei of China. Out of 24 endophytes investigated, 12 strains can produce in vitro substances that are inhibitory to all or a few of tested phytopathogens whereas the rest yielded nothing active,...

Author(s): Zhengyan Guo, Rimao Hua, Yu Bai, Xiaowen Wu, Haiqun Cao, Xuede Li, Xiangwei Wu and Jun Tang

July 2011

Staphylococci and other selected microbiota associated with indigenous traditional beer

The nature and origin of indigenous traditional beer, makes it is prone to spoilage by a variety of microbiota in particular post-fermentation. In this study, samples of commercially and homebrewed indigenous traditional beer were collected using sterile sampling Whirl-pak® bags from local informal brewers in typical marginal urban settlements of South Africa.  Both commercially and homebrewed...

Author(s): J. F. R. Lues, B. K. Ikalafeng, M. Maharasoa, K. Shale, N. J. Malebo and E. Pool

July 2011

Molecular detection of Vibrio spp. in lobster hemolymph

The occurrence of various Vibrio species in lobster hemolymph from the Persian Gulf was studied. A total number of 60 lobsters (Panulirus homarus) were caught from south coast of Iran and were studied to identify Vibrio spp. in hemolymph. Four Vibrio species includingVibrio alginolyticus, Vibrio vulnificus, Vibrio harveyi and Vibrio mimicus were identified using...

Author(s): Mehdi Raissy, Hassan Momtaz, Manouchehr Moumeni, Mahsa Ansari and Ebrahim Rahimi

July 2011

Serum paraoxonase and arylesterase activities and oxidant status in patients with brucellosis

The aim of this study was to the investigate the effect of brucellosis infection on paraoxonase-1 (PON1) activity and oxidative status in patients with brucellosis. Sixty patients with brucellosis (47 acute, 13 subacute) and the 67 healthy controls were used in the study. Serum PON1 and arylesterase activities, total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total free...

Author(s): Hasan Karsen, Mustafa Kasım Karahocagil, Hayrettin Akdeniz, Mehmet ReÅŸat Ceylan, Irfan Binici, Sahbettin Selek and Hakim Celik

July 2011

Antibiotic resistance of some lactobacilli isolated from the gut microflora of broiler

To evaluate the impact of the antibiotic use in poultry and the development of the resistance in the gut microflora of broiler. Microbiological methods were used to investigate the susceptibility of ten lactobacilli stains isolated from digestive tract of broiler. The isolates were tested against the ten most used antibiotics in veterinary and human medicine in Algeria. The isolates exhibited high resistance to all...

Author(s): Behira Belkacem and Kihal Mebrouk