African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 4 March, 2015; 9(9)

March 2015

A review of perception and myth on causes of cholera infection in endemic areas of Nigeria

Cholera epidemic is a recurrent disease in developing countries with poor environmental sanitation and inadequate supply of potable water. In endemic areas of West Africa, opinions and myths surrounding the real causes of cholera diseases have given rise to varying methods of control. This review gives an insight into these various opinions and myths. Some of these are that magico-religious factors, witchcraft, eating...

Author(s): Innocent AO Ujah, Francisca Nwaokorie, Adeniyi Adeneye, David Oladele, Tajudeen Bamidele, Sola Musa, Kehinde Akinsinde, Jerry Iwuora, Mike Ochoga and Stella Smith

March 2015

Impact of purified human milk oligosaccharides as a sole carbon source on the growth of lactobacilli in in vitro model

Recently, there is a growing interest in the use of oligosaccharides as prebiotics in order to modulate the growth of beneficial gut microbiota. It is known that human milk is a rich source of complex oligosaccharides. This paper reports the in vitro growth of six strains of lactobacilli in media containing purified human milk oligosaccharides (HMOs) obtained from breast milk. Based on the evaluation of bacterial...

Author(s): Gabriela KRAUSOVA, Vojtech RADA, Petr MARSIK, Sarka MUSILOVA, Roman SVEJSTIL, Vladimir DRAB, Ivana HYRSLOVA and Eva VLKOVA

March 2015

Microbiota of two species of commercially important fish in the Amazon region (Belém-Pará-Brazil): Butterfly peacock bass (Cichla ocellaris) and piramutaba (Brachyplatystoma vailantii)

The Amazon offers great potential for fishery activities but the fish fauna’s specific microbiota is not yet known. This paper identified the bacterial flora composition and the influence of this process on the microbiological spoilage in economically important fish species in the Amazon region: butterfly peacock bass (Cichla ocellaris) and piramutaba (Brachyplatystoma vailantii). To this end, microbiological...

Author(s): Evelyn Ivana Trindade Damasceno, Lauana Natasha da Gama Pantoja, Hamilton Mendes de Figueiredo, Luiza Helena Meller da Silva and Antônio Manoel da Cruz Rodrigues

March 2015

Secondary metabolites from the culture broth of Lactarius quieticolor growing in a bioreactor

In this work, the main secondary metabolites in Lactarius quieticolor cultivated in a bioreactor were determined to find a possible use for its broths, which are eliminated as waste products after fermentation. The broths were extracted for adsorption in solid phase using amberlite XAD-2 and solid-phase extraction (SPE), and the extracts were analyzed by gas chromatography-mass spectrometry. The main metabolites...

Author(s): Daniel Chávez, Víctor Hernández, Ángela Machuca, Götz Palfner and José Becerra

March 2015

Optimization of L-glutaminase synthesis by Aspergillus oryzae NRRL 32657 in submerged culture

Various fungal strains have been screened for L- glutaminase synthesis.  Aspergillus oryzae NRRL 32657 proved to be the highest producer (24.19 U.ml-1) with the largest pink zone diameter (8 mm). Production of L- glutaminase (E.C. 3.5.1.2. L-glutamine amidohydrolase) by A. oryzae in batch submerged system was optimized. Several factors: incubation period, source and concentration of carbon, source and concentration...

Author(s): Ahmed M. Alian, Wael A. Bazaraa, Nagwa M. El- Shimi and Reda M. Mohamed

March 2015

Occurrence of Brucella abortus, Leptospira interrogans and bovine herpesvirus type 1 in buffalo (Bubalus bubalis) herd under extensive breeding system

The current study aimed to determine the occurrence of Brucella abortus, Leptospira interrogans and BoHV-1 in buffalo (bubalus bubalis) herd under extensive breeding system. In order to perform the study, blood samples were collected from 306 female buffaloes belonging to a rural property located in São Mateus County – MA. The samples were tested for the presence of specific antibodies. The number of...

Author(s): Osmana Severo Carvalho, Ludmila Nayara R. Gonzaga, Aline Saldanha Albuquerque, Danilo Cutrim Bezerra and Nancyleni Pinto Chaves

March 2015

Survival analysis for estimating risk factors in incubation period of Didymella pinodes on pea (Pisum sativum L.)

Ascochyta blight caused by Didymella pinodes (Berk. et Blox.) Vestergr. is one of the most devastating diseases, causing severe damage in pea. A new statistical approach based on factor risk using non- parametric and semi parametric survival analysis was used in this study. Different hypotheses dealing with factors that might influence the incubation period were tested. Survival analysis using Kaplan-Meier estimates and...

Author(s): Setti Benali, Bencheikh Mohamed and Henni Jamel Eddine

March 2015

Evaluation of diagnostic polymerase chain reaction (PCR) for the detection of Escherichia Coli, Staphylococcus aureus and Bacillus cereus in cheese

Polymerase chain reaction (PCR) assay was used to detect pathogenic bacteria in white cheese samples using suitable primers which are based on specific genes. A total of 70 samples of white cheese were collected from different parts of Baghdad for detection of the pathogens, Escherichia coli, Staphylococcus aureus and Bacillus cereus. Cheese was made ​​locally from different sources of milk (buffalo, cows and...

Author(s): Kamil Mutashar M. Al Jobori, Ali Hashim Aboodi and Majida Jumaah Darweesh

March 2015

Comparison of Brucella agar, CITA and Farrell media for selective isolation of Brucella abortus from semen of bovine bulls

Brucellosis remains as a public health concern worldwide. In domestic animals, the disease is characterized by reproductive disorders in male and female. Besides extensive use of serological tests and recent development of molecular biology techniques, microbiological culture of Brucella species is yet considered a “gold standard” method for diagnosis. Here, semen of 335 bovine bulls was subjected...

Author(s): Gerado de Nardi Júnior, Jane Megid, Acácia Ferreira Vicente, Fernando José Paganini Listoni, Fábio Morato Monteiro, Gustavo Henrique Batista Lara, Rodrigo Garcia Motta, Marcelo George Mungai Chacur and Márcio Garcia Ribeiro

March 2015

Random amplified DNA polymorphism of Klebsiella pneumoniae isolates from Mansoura University Hospitals, Egypt

Klebsiella pneumoniae is one of the major causes of nosocomial infection. Understanding the route and dynamics of dissemination in the outbreaks of infections relies on using accurate typing methods. This is achieved through molecular typing using random amplified polymorphic DNA (RAPD) analysis technique in correlation to the infection source and antibiotic resistance. In this study, a total of 300 clinical isolates...

Author(s): Ramadan Hassan, Rasha Barwa, Mona I. Shaaban and Lamiaa Adel

March 2015

Production of cellulases from Humicola fuscoatra MTCC 1409: Role of enzymes in paddy straw digestion

Cellulases were produced from fungus Humicola fuscoatra MTCC 1409 by solid state fermentation under different cultural conditions viz. pH, incubation temperature, inoculum size and days of incubation in order to optimize the conditions for maximum enzyme production. The potential of cellulase pretreatment to increase the digestibility of paddy straw was also ascertained. Maximum enzyme production was achieved at pH 6.0...

Author(s): Baldeep Kaur, Surekha Bhatia and Urmila Phutela

March 2015

Effect of nitrogen fertilization associated with diazotrophic bacteria inoculation on nitrogen use efficiency and its biological fixation by corn determined using 15N

The use of plant growth-promoting diazotrophic bacteria as an alternative to increase nitrogen (N) availability and contribute to its use by corn can be a less costly and ecologically more viable option. Thus, this study aimed to evaluate the effects of the inoculation with Azospirillum brasilense and Herbaspirillum seropedicae in association with N fertilization on the N use efficiency and its biological fixation by...

Author(s): Érica de Oliveira Araújo, Marcio Reis Martins, Antonio Carlos Tadeu Vitorino, Fabio Martins Mercante and Segundo Sacramento Urquiaga

March 2015

Molecular typing of Salmonella enterica serovars Typhimurium and Enteritidis isolated from Taif area of Saudi Arabia by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)

Twenty three isolates of Salmonella enterica representing two serovars, Salmonella Typhimurium and Salmonella Enteritidis isolated from three hospitals in Taif province, Kingdom of Saudi Arabia, were allocated to 23 genomic types using random amplified polymorphic DNA RAPD-PCR employing four 10-mer arbitrary primers. Jaccard's similarity coefficients were used to reveal the genetic diversity among isolates. Random...

Author(s): Ohud M. Al-Harthi, Hayam S. Abdelkader, and Eman M. Halawani

March 2015

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

Extensive use of fluoroquinolone antibacterial in clinical practice has been associated with increasing frequency of quinolone-resistant Escherichia coli strains. In the current study, a total of 80 E. coli clinical isolates from Mansoura hospitals patients in Egypt were studied for antibacterial susceptibility pattern against 15 different antibacterials. These strains were tested for quinolones resistance by minimum...

Author(s): Mohamed Mohamed Adel El-Sokkary and Eman Salama Abdelmegeed