July 2012
Single primer designed for detection of Shigella species
In infectious disease diagnosis prior to treatment, identification of the causative agent plays a crucial role. Polymerase chain reaction (PCR) has paved a molecular way for specific species identification. In this study, we have attempted to design a single common primer to identify the causative agent for shigellosis - Shigella spp. Bioinformatic tools like CLUSTALX for multiple sequence...
July 2012
Effects of timing of drought stress on pomegranate seedlings (Punica granatum L. cv ‘Atabaki’) to exogenous spermidine and putrescine polyamines
Plant growth and productivity is adversely affected by nature’s wrath in the form of various biotic and abiotic stress factors. Drought stress causes reductions in growth rate, along with a suite of metabolic changes. The initial reduction in shoot growth is probably due to hormonal signals generated by the roots. Different foliar treatments of spermidine and putrescine polyamines were applied...
July 2012
Floral differentiation and development in early, middle and late blooming almond cultivars
Flower initiation and development in almond (Prunus amygdalus L.) were examined with the objectives of determining the timing of floral developmental stages and achieving a better understanding of the morphological changes during flower formation at the apex of axillary buds of early, middle and late blooming almond cultivars. In almond trees, bud samples were taken every 10 days from May 31, 2007....
July 2012
CTX-M from community-acquired urinary tract infections in Algeria
Thirty-eight Enterobacteriaceae isolates including seventeen Klebsiella pneumoniae,thirteen Escherichia coli and eight Enterobacter cloacae resistant to broad-spectrum cephalosporins were recovered from community-acquired urinary-tract infections at four private-laboratories in Bejaia, Algeria, between March 2007 and May 2009. Antimicrobial susceptibility testing of these...
July 2012
Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of multiple legume species native of Fez, Morocco
Root-nodulating bacteria were isolated and characterized from grain and forage legumes growing in Fez regions. A total of 110 Rhizobium strains were characterized on the basis of morphological, cultural, and phenotypical properties. Phenotypic characteristics studied included growth rates in various media, colony morphology, tolerances to extremes of temperature, salt and pH and resistance to different...
July 2012
Coexistence of armA and genes encoding aminoglycoside-modifying enzymes in Acinetobacter baumannii
A total number of 104 Acinetobacter baumannii isolates obtained from a teaching hospital were studied for the genetic basis of their resistance to aminoglycosides. The rate of aminoglycoside-resistant A. baumannii was 76.9% (80/104), and 66.25% (53/80) aminoglycoside-resistant isolates were high-level resistant to either aminoglycosides including amikacin, gentamicin and tobramycin...
July 2012
Incidence and prevalence of antimicrobial resistant Vibrio cholerae from dairy farms
The study was aimed to evaluate the food safety status of dairy farms. Sample collection was carried out from four different dairy farms from Meerut city and Babugarh cantt, UP, India. The extent of prevalence for Vibrio cholera was assessed in raw milk and soil samples. Bacterial isolates were identified through biochemical and immunological characterization. The degree of susceptibility of these...
July 2012
Characterizations of selected alpha-amylase producing bacterial strains isolated from soil samples of the alluvial plains of Southern Brahmaputra corridor of Assam
Alpha amylase is an important enzyme used in different industries, which degrades starch into smaller disaccharides. Extra cellular alpha-amylase producing bacteria were screened based on their biochemical properties from soil samples of different depth collected from Southern Brahmaputra corridor. A total of twenty strains were screened the rich alluvial soil from the southern corridor of the river...
July 2012
Soil hyphomycetes population dynamics in disturbed and undisturbed tropical soils of North-eastern India
Soil Hyphomycetes diversity from virgin forest floor (VFF) and cultivated rice field (CRF) of the Upper Brahmaputra Valley was studied in four different seasons during 2008-2009. The average fungal population in the VFF and CRF were 0.70–2.20×104 and 0.40–1.8×104 CFU/g, respectively. A total of 36 fungal species were isolated from both the sites of...
July 2012
Seroprevalence, geographic distribution and risk factor analysis of Borrelia burgdorferi sensu lato in naturally exposed dogs of Iran
Lyme borreliosis is a worldwide tick-borne zoonotic disease caused by spirochetes of theBorrelia burgdorferi sensu lato group. The aims of the current study were to determine seroprevalence of lyme borreliosis caused by B. burgdorferi sensu lato among dogs andto analyze its environmental risk factors in Iran. Blood samples were collected from...
July 2012
Enhanced photo-fermentative hydrogen production from different organic substrate using hupL inactivated Rhodopseudomonas palustris
Hup hydrogenase in Rhodopseudomonas palustris is a type of hydrogen-uptake hydrogenase that mediates hydrogen fixation and utilization. Active Hup hydrogenase is known to inhibit the hydrogen production capacity of R. palustris. In this study, a Hup hydrogenase inactivated strain, â–³hupL CN, was constructed by deleting the hupL gene. Photo hydrogen production was enhanced in the mutant...
July 2012
Multivariate analysis linkage of phyllospheric microbial community of transgenic cotton from SGK321 to Cry1Ac: A temporal expression dynamics
The effects of transgenic plant on ecological and food biosafety have attracted considerable attention recently due to the exploding number of commercialization genetic modified plants. Here, we examined the changes of bacterial and fungal community compositions in the phyllosphere of Cry1Ac Bacillus thuringiensis (Bt) cotton SGK321 by molecular fingerprint method at four major growth stages. The...
July 2012
Prevalence of giardiasis in selected dairy cattle farms in Lahore, Pakistan
Fecal samples of 720 cattle were collected from four different areas of Lahore, that is,Military dairy farm, Government dairy farm, Gawala dairy colonies and House hold dairy cows. All samples were purified and cysts were quantified microscopically. DNA was extracted from the purified cysts and was amplified through polymerase chain reaction. The overall prevalence was 31.11% (224/720), where high prevalence...
July 2012
Bactericidal activity of various antibiotics versus tetracycline-loaded chitosan microspheres against Pseudomonas aeruginosa biofilms
The discovery of biofilms in 1980’s has brought much interest to the study of the contribution of bacterial biofilms with many recurrent and chronic infectious diseases. In this study, we evaluated the utility of chitosan microspheres in delivering antibiotic in dosage form that could be effective against Pseudomonas aeruginosa (P. aeruginosa) biofilms. P. aeruginosa isolates were...
July 2012
In vitro and in vivo studies of antibacterial effect of ceftriaxone moxifloxacin combination against methicillin resistant Staphylococcus aureus biofilms formed on biomedical implants
Methicillin resistant Staphylococcus aureus (MRSA) is the etiologic agent of a wide range of diseases worldwide including the Middle East. Biofilm production is an important virulence attribute in the pathogenesis of device-related MRSA infection. Our aim was to study the bactericidal effect of cephalosporin/fluoroquinolone combinations against MRSA biofilm in vitro and in...
July 2012
Detection of Salmonella strains in clinical samples from Saudi Arabia by invA and hilA polymerase chain reaction (PCR)-based assays
In this study, 23 Salmonella isolates were detected by polymerase chain reaction (PCR) from human fecal samples obtained during 2010 from local hospitals and clinical laboratories in Kingdom of Saudi Arabia (KSA). The bacteria were cultured, serotyped and biochemically characterized by the analytical profiling index (API 20E). The invA and hilA gene primers were selected...
July 2012
Genetic diversity analysis of Ganoderma species and development of a specific marker for identification of medicinal mushroom Ganoderma lucidum
The genetic diversity of 59 Ganoderma species from various regions was determined by different molecular markers, including the internal transcribed spacer (ITS) rRNA and a partial β-tubulin gene, as well as randomly amplified polymorphic DNA (RAPD) analysis.The size of the ITS rRNA gene regions from different Ganoderma species varied from 625 to 673 bp, and that of the partial...
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