African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 9 August, 2012; 6(30)

August 2012

A sustainable laboratory approach for contagious bovine pleuropneumonia (CBPP) monitoring in Nigeria: Comparison between two serological tests in an endemic area complimented with post mortem lesions

A study was carried out to develop a sustainable laboratory approach for contagious bovine pleuropneumonia (CBPP) monitoring using two serological tests and examination of lungs from slaughtered cattle in Maiduguri, Nigeria. A total of 500 lungs from 340 males and 160 females, aged between 1 and 5 years were examined at post-mortem (PM) for pneumonic lesions characteristic of CBPP. Out of this number, 92 (18.4%) showed...

Author(s): Egwu G. O., Adamu M., , Mshelia G. D. and Bukar-Kolo Y. M.

August 2012

Effects of flashing light from light emitting diodes (LEDs) on growth of the microalga Isochrysis galbana

The growth of Isochrysis galbana was examined under flashing- and non-flashing-light conditions. Cell densities were higher under flashing light at 104 μmol photons m–2 s–1than at 60 μmol m–2 s–1. At high daily irradiance, cell densities were significantly higher under flashing light at most frequencies than in control cultures under non-flashing light. Final cell...

Author(s): T. Yago, H. Arakawa, K. Fukui, B. Okubo, K. Akima, S. Takeichi, Y. Okumuraand T. Morinaga

August 2012

Investigation on clinical healthy swine carrier status of Streptococcus suis in Hebei Province of China

A total of 600 samples of nose swabs collected from Hebei Province, China, were examined by polymerase chain reaction (PCR) for the presence of Streptococcus suis in healthy swine and the serotype were identified. Results showed that 148 strains (24.67%) of 600 tested were positive with S. suis, including 27 strains (18.24%) were identified to be type 7, 24...

Author(s): Ping Rui, Zeng-Jun Ma, Qiu-Yue Wang, Xiang-zhai Zhang, Jin-Xia Wang, Yan-Ying Zhang and Hai Fang

August 2012

Epidemiology, virulence and public health significance of Yersinia enterocolitica in drinking water

Yersinia enterocolitica, an important food and water-borne enteropathogen cause acute diarrhoea, terminal ileitis and mesenteric lymphadenitis. This study investigated the occurrence, antibiotic sensitivity and virulence factors of yersinial isolates from drinking water samples from Ludhiana, Punjab, India. A total of 418 drinking water samples from various water utilities were analyzed for occurrence of Y....

Author(s): Pandove G., Sahota P., Verma S. K., Mahajan V., Singh . D. and Singh J. P.

August 2012

Characterization of urease enzyme from marine bacterium Klebsiella species

The rapidly increasing importance of enzyme urease applications has drawn attention to the need of enzyme study. It prompted the present study to hunt a promising bacterial strain with the desired nature from the Porto Novo coast. Urease enzyme is used in diagnostic kits for measuring blood urea, removal of urea from alcoholic beverages, urease conductometric biosensors for detection of heavy metal ions, etc. In our...

Author(s): Senthil Balan S., Fazila Fathima and Jayalakshmi S.

August 2012

Production optimization and characterization of a low molecular weight bacteriocin from Lactococcus lactis subsp. lactis

Lactococcus lactis subsp. lactis is very important as starter culture of cheese and yogurt production. A variety of bacteriocins ranging the molecular weight 3 to 10 have been reported from L. lactis under different growth conditions. In our study, we have optimized the growth of L. lactis for the bacteriocin production. Growth kinetics of these bacteria was studied in accordance...

Author(s): Mehwish Aslam, Muhammad Shahid, Fayyaz ur Rehman, Mian Anjum Murtaza, Sumaira Sharif, Asia Ata and Saba Noor

August 2012

Effect of vitamin E on seedling growth in parsley (Petroselinum sativum L.)

In order to observe the effect of vitamin E on seedling growth in parsley (Petroselinum sativum L.), this experiment was conducted in 2011 at Islamic Azad University, Shahr-e-Qods Branch, in Tehran by a completely randomized design with four replications. The factor was use of vitamin E (0, 1, 2, 3, 4 and 5 percentage). The results obtained show that the antioxidant (vitamin E) had...

Author(s): Hossein Aliabadi Farahani, Davud Karimian and Kasra Maroufi

August 2012

Influence of carbon source on the production of exopolysacharides by Rhizobium sullae and on the nodulation of Hedysarum coronarium L. legume

The exopolysaccharides (EPS) produced by species of the Rhizobium genus are essential for establishing symbiotic nitrogen fixation with legumes. Rhizobium strains were isolated from the wild plant Hedysarum coronarium L. collected in Constantine, Algeria. The effect of carbon source on the production of EPS and the infectivity of the bacteria were studied for the...

Author(s): Gharzouli Razika, Benahmed Amira, Benhizia Yacine and Benguedouar Ammar

August 2012

Homology modeling of bacterial endotoxin

Bacillus thuringiensis (or Bt) is commonly used as a biological pesticide; alternatively, the Cry toxin may be extracted and used as a pesticide. It occurs naturally in the gut of caterpillars of various types of moths and butterflies, as well as on the dark surfaces of plants. Bt crystals, sometimes referred to as insecticidal crystal proteins (ICP), are protein crystals formed during sporulation in some Bt...

Author(s): Natarajan Valarmathy#, Nadarajan Saravanan Prabhu#, Kanagavel Deepankumar, Ravikumar Sambandam, Abdulrahman Alsenaidy and Niraikulam Ayyadurai,

August 2012

The reliable and rapid polymerase chain reaction (PCR) diagnosis for Xanthomonas axonopodis pv. punicae in pomegranate

Bacterial blight is a major disease in pomegranate (Punica granatum) cultivation in India threatening the export potential of this important fruit crop. The disease is caused by a yellow pigmented, Gram negative, rod shaped bacterium, Xanthomonas axonopodis pv.punicae. We developed a polymerase chain reaction (PCR) based detection technique for this blight pathogen using primers designed...

Author(s): Kalyan K. Mondal, T. P. Rajendran, C. Phaneendra, C. Mani, Jyotsana Sharma,Richa Shukla, Pooja, Geeta Verma, R. Kumar, D. Singh, A. Kumar, A. K. Saxena and R. K. Jain

August 2012

The effect of solar ultraviolet radiation and ambient temperature on the culturability of toxigenic and non-toxigenic Vibrio cholerae in Pretoria, South Africa

Although solar disinfection (SODIS) is known to be one way of controlling waterborne diseases like cholera, the potential impact that this technology can have in resource poor areas is increasingly being considered as a potential component in water treatment for poor and rural communities and as a means to alleviate the burden of disease. In this study, comparative growth analysis was conducted on three Vibrio...

Author(s): C. C. Ssemakalu, , M. Pillay, and E. Barros

August 2012

Comparison of bacterial diversity in large intestine of Xiangxi yellow cattle (Bos taurus) associated with different diet: Fresh Miscanthus sinensis and mixed forage

Ruminants harbour a complicate ecosystem consisting mostly of microorganisms which have the ability to digest the fibrous cell walls of plant materials from host's food. To discover the novel enzyme from ruminant's digestive tract for exploration of the new biofuel plant-Miscanthus sinensis, we comparatively analyzed the distribution of microbiome in the large intestine of two groups of local cattle fed with...

Author(s): Yadan Li, Lijuan Hu, Gaoshang Xue, Huhu Liu, Hui Yang, Yunhua Zhu,, Yun Tian, and Xiangyang Lu,

August 2012

Mutations of hepatitis B virus S gene by hepatitis B immunoglobulin administration in late pregnancy

The safety of hepatitis B immunoglobulin (HBIG) administration in late pregnancy is still controversial, especially the hepatitis B virus (HBV) mutation-generating effects of HBIG. In order to clarify the association between HBV S gene mutations and HBIG administration in late pregnancy, 106 HBV carrier mothers and 60 newborns were recruited for the study. Peripheral blood specimens were collected from mothers before...

Author(s): Lu Gan#, Dongyang Liu, #, Ming Wei, Caiping Ke, Xiaomei Tang,  and Xiaomin Xiao

August 2012

Identification of common outer membrane proteins of Porphyromonas gingivalis by shotgun proteomics

P. gingivalis is considered a major pathogen for periodontitis. In the process of identifying candidate antigens for a vaccine, two shotgun proteomics workflows by differentsolubilization and digestion methods were investigated in identifying outer membrane proteins (OMPs) of P. gingivalis. The first approach utilized the direct organic/detergent solubilization and separation in the studies of P....

Author(s): Jin Yang, Yuanyuan Sun, Junyi Sun, Jianzhong Gou and Ang Li

August 2012

Age-dependent response to CpG oligodeoxynucleotides against organ invasion of Salmonella enteritidis in broiler chickens

Bacterial infections cause significant economic losses in the broiler industry throughout the production cycle. It is known that CpG-ODN stimulated innate immune activities and enhanced the resistance to infectious pathogens in chickens. In this study, the effectiveness of CpG-ODN was evaluated in organ colonization by Salmonella enteritidisat different ages of chickens. Pre-treatment of 2-day old chickens with...

Author(s): Vahideh Shahrokhi, Mehrnaz Rad and Gholam Ali Kalidari

August 2012

Indole, a bacterial signaling molecule, exhibits inhibitory activity against growth, dimorphism and biofilm formation in Candida albicans

A wide variety of signaling molecules produced by bacteria may influence physiology of neighboring microorganisms. Indole, a small molecule secreted by bacteria is known to control growth, physiology as well as biofilm formation in various bacteria. Effects of indole on eukaryotic microorganisms are not known. The objective of this study was to analyze response of Candida albicans, commensal yeast of the humans, to...

Author(s): Jayant S. Raut, Ravikumar B. Shinde and Mohan S. Karuppayil

August 2012

New record of Ramularia leaf spot on Aristolochia punjabensis L. in Pakistan

During September to November 2011, in the course of routine sample collection, a leaf spot disease was observed on Aristolochia punjabensis in Lahore, Pakistan. The fungus appeared as a white, fuzzy mold covering the leaf spot on both surfaces of leaf, however, more pronounced on lower side. On the basis of symptoms, fungal morphology and completion of Koch’s postulate, the pathogen was identified...

Author(s): Irum Mukhtar, Ibatsam Khokhar, Mohammad Saleem Haider and Sobia Mushtaq