September 2013
Metabolism and degradation of glyphosate in aquatic cyanobacteria: A review
Use of glyphosate (N-phosphonomethylglycine), a broad-spectrum, non-selective, post-emergence herbicide has been increased steadily with the introduction of genetically modified glyphosate-resistant crops. Increased reliance on herbicides for suppressing weeds and aggressive marketing have also contributed substantially to rising demand for glyphosate. Degradation of...
August 2013
Comparison of conventional method and automatized vitek sysytem in the detection of extended-spectrum beta-lactamase in Escherichia coli and Klebsiella pneumoniae isolates
Beta-lactamase production is the most important mechanism in resistance development of Gram negative bacteriae. Extended spectrum beta-lactamases (ESBL) enzymes hydrolyse penicillin and cephalosporins. Many ESBLs are derived by genetic mutation from naturally occuring enzymes. ESBL can be detected by phenotypic and genotypic methods. In our study, we compared the ESBL detection performance of...
August 2013
Heteroresistance to fluconazole among isolates of Cryptococcus neoformans in Northern Thailand
We had determined that 190 Cryptococcus neoformans isolates (104 clinical and 86 environmental isolates from northern Thailand) were all susceptible to amphotericin B, fluconazole, itraconazole and ketoconazole. Of 14 selected isolates, one clinical isolate (CN4969) exhibited heterogeneity in fluconazole resistance, which produced two subpopulations of the fluconazole-resistant (CN4969HR) and...
August 2013
OSOM BV blue test: A new point-of-care test for diagnosing bacterial vaginosis and its comparison with Gram staining
Bacterial vaginosis (BV) is the most common cause of abnormal vaginal discharge in females of reproductive age group which predisposes them to other sexually transmitted diseases including human immunodeficiency virus (HIV) and various obstetric complications. In the present study, efficacy of OSOM BV Blue test was evaluated for diagnosing bacterial vaginosis in comparison with Gram staining. The study...
August 2013
Sufficient intracellular survival capability is essential for induction of protective immune response by the Brucella live attenuated vaccine M5-90
The fact that live attenuated vaccine but not killed Brucella cells could induce protective immune response against Brucellosis implied that survival capability in host is important for the immune response induction. Member of the Omp25 protein family are shown to be important for Brucella virulence and immunological regulation. Omp25 mutants of virulent strains shows reduced virulence and could...
August 2013
Evaluation of phenol degradation by effective microorganism (EM) technology with EM-1
Effective microorganisms (EM) are a culture of co-existing beneficial micro-organisms predominantly consisting of lactic acid bacteria, photosynthetic bacteria, yeast, fermenting fungi and actinomycetes that are claimed to enhance the decomposition of organic matter which in turn improves soil fertility and toxic pollutants including petroleum hydrocarbons, among others. In the present study, EM-1 commercial formulation...
August 2013
Microbiological safety and quality assessment of a very appreciate traditional ready to eat plantain food, sold in retails markets
The microbiological quality of “Dockounou”, a traditional appreciate ready to eat plantain food marketed in Côte d’Ivoire was investigated. Microbial load was estimated by plate count using decimal dilution method. Culture medium was made selective depending on the species. Total aerobic mesophilic bacteria on principal component analysis (PCA) ranged from 32.104 to 44.104 cfu/g while...
August 2013
Determination of typical house flora during production process of the Petrovac Sausage (Petrovská klobása)
In the meat sector, different crises, but also the recurring food-poisoning cases, have undermined public confidence in intensive or industrial meat producing systems. Consumers are, therefore, turning to traditional products. Traditional fermented dry sausages account for a significant part in such a domain. Traditional dry sausages rely on natural contamination by environmental flora. This microbiota...
August 2013
Physicochemical and antimicrobial properties of Tunisian honeys: Honey inhibited the motility of bacteria
Honey is a sweet flavourful product which has been consumed as a high nutritive value food. The present study aimed to characterize 12 samples of honeys collected from the North of Tunisia in respect to their floral origins. pH, hydroxyl methyl furfural, moisture content, ash, acidity, electrical conductivity, reducing sugars, apparent sucrose and total phenolic contents, were the parameters analysed in...
August 2013
Bacterial diversity of aMasi, a South African fermented milk product, determined by clone library and denaturing gradient gel electrophoresis analysis
In the present study, we investigated the bacterial diversity of aMasi, a traditional South African fermented milk product, by 16S rRNA clone library and Denaturing Gradient Gel Electrophoresis (DGGE) analysis. Two hundred and eighty two clones from clone library were isolated and identified from aMasi, prepared from the milk of four cows from one herd in the EkuPindiseni Community, North West of...
August 2013
Effect of propionic acid and Lactobacillus plantarum UFLA SIL 1 on the sugarcane silage with and without calcium oxide
Alkaline agents reduce the fiber content, but results in a buffering of the silage that can benefit the growth of undesirable micro-organisms. The association of alkaline agents and additives capable of inducing a decrease in the initial pH may be interesting. Our aim was to evaluate the effects of a wild strain of Lactobacillus...
August 2013
Microbiological quality of Ayib, traditional Ethiopian cottage cheese, in Jimma area, South-West Ethiopia
This study was conducted on randomly selected 150 samples of soft cheese (Ayib)collected from five vendors of open market places at Jimma town and its surrounding districts: Dedo, Seka, Serbo and Yebu markets to determine microbial quality. Samples were taken three times from the same vendors at different market days with sterile screw cap bottles while categorized into two: leselasa (soft)...
August 2013
Yeasts diversity in Brazilian Cerrado soils: Study of the enzymatic activities
A total of 307 yeasts strains were isolated from native Cerrado (Brazilian Savannah) soils collected at Passos, Luminárias and Arcos areas in Minas Gerais State, Brazil. The soils were chemically characterized. Ten yeast genera (Candida, Cryptococcus, Debaryomyces, Kazachstania, Kodamaea, Lindnera, Pichia, Schwanniomyces, Torulaspora and Trichosporon) and 23 species in both rainy and dry...
August 2013
Enterococci inoculated silages: Effect on rumen fermentation and lipid metabolism in vitro
The effect of providing diets containing grass silage (12.8 g of DM/day) and barley grain (3.2 g of DM/day) or maize silage (12.8 g of DM/day) and lucerne hay (3.2 g of DM/day) into RUSITEC (rumen simulation technique) in which the grass or maize were uninoculated (GS, MS) or inoculated with strains Enterococcus faecium EF2/3s (GS + EF2/3s, MS + EF2/3s) or E. faecium EF26/42 (GS + EF26/42, MS +...
August 2013
Isolation and characterization of actinobacteria from Lake Magadi, Kenya
Actinobacteria are ubiquitous and have been detected in terrestrial and aquatic ecosystems. Although, various studies have focused on the ecology of this phylum, data on the ecology of actinobacteria endemic to soda lakes are scanty. This study aimed to determine actinobacterial diversity in Lake Magadi. Four different sampling points were selected randomly within the lake to cover a broad range of...
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