African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 9 December, 2011; 5(29)

December 2011

Sequencing of 16S rRNA gene for identification of Staphylococcus species in water sample

  A bacterial strain Bz19 was isolated from a water sample collected from river Gomati at the Indian city of Lucknow. We characterized the strain using 16S rRNA gene sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Staphylococcus. The closest phylogenetic relative was Staphylococcus arlettae with 99% 16S rRNA gene sequence similarity. It...

Author(s): Priyanka Shukla, Shipra Srivastava, Rajeev Srivastava and Anil Kumar Rawat

December 2011

Antibacterial profile of Jatropha curcas latex extracts against selected human pathogenic bacteria

  Jatropha curcas is a drought-resistant tree belonging to the family Euphorbiaceae. The latex of J. curcas has been traditionally used to heal wounds and has reported anti-coagulant and coagulant properties. In this study, fractions of the methanol, aqueous, ethyl acetate and hexane extracts from the Jatropha latex were tested for antibacterial properties against nine...

Author(s): Z. Suhaili, C. C. Yeo, H. N. Yasin, N. A. Badaludin and Z. A. Zakaria

December 2011

Application of saline water and herbicides as a method for weed control in the tropical turfgrass: Its impact on nutrient uptake and soil microbial community

  A field study was conducted at Universiti Putra Malaysia with the aim to find out a suitable environment friendly weed control method for turfgrass species in combination with different levels of sea water and herbicides. A number of three turfgrass species (Paspalum vaginatum, Zoysia japonica and Cynodon dactylon ‘satiri’) and five associative weed species (Eragrostis...

Author(s): Md. Kamal Uddin, Abdul Shukor Juraimi, Mohd. Razi Ismail, Umme Aminun Naher, Radziah Othman and Anuar Abdul Rahim

December 2011

Evaluation of some antagonistic bacteria in biological control of Gaeumannomyces graminis var tritici causal agent of wheat take-all disease in Iran

  Take-all is a disease of wheat root caused by the soil borne fungus, Gaeumannomyces graminis (Sacc) Von Arx and Oliver var tritici Walker (Ggt) all over the word. There has been considered biological control using the microorganisms departed from suppressive soils or wheat roots, widely. In this study, strains of Bacillus (B. subtilis and B....

Author(s): Elahe Babaeipoor, Soheila Mirzaei, Younes Rezaee Danesh, Amir Arjmandianand Mehrdad Chaichi

December 2011

A report and analysis on the sampling and inspection of 50692 drugs

  Ensuring safe use of drugs has always been of high concern to the Chinese central and local Governments. Comprehensive and accurate evaluation of drug quality status is of great importance. This paper systematically and realistically evaluates the drug sampling inspection reports in Liaoning Province in recent three years, carefully analyzes the causes of drug disqualification and proposes some suggestions...

Author(s): Wang Ming-Dong, Li Ye, Han Ling, Pan Ying and He Xiao-Xia

December 2011

Simultaneous occurrence of saxitoxins and biogenic amines in mackerel fish

Seafood toxins resulted from a wide variety of naturally occurring or man-made toxic compound may cause a serious hazard to consumers. Biogenic amines that cause histamine poisoning and (STX) that cause paralytic shellfish poisoning (PSP) are among these seafood toxins that may occur in mackerel fish and their presence may be affected by seasonal and locality variation. The presence of STX and biogenic amines together...

Author(s): M. N. Gomaa, K. M. Soliman, M. A. Embaby, and A. M. Ayesh

December 2011

Expression of chitinase and chitin binding proteins (CBP’s) by Listeria monocytogenes J0161 in biofilm and Co-culture broths

  Listeria monocytogenes is a dreaded pathogen colonizes to form biofilm in the natural environments and in food processing facility. Biofilm state makes the bacterium resistant to antimicrobial agents. L. monocytogenes, an intracellular parasite also colonizes the mammalian epithelial cells in the gut. Chitinase and other chitinolytic proteins expressed by L. monocytogenes contribute to the...

Author(s): Prem Saran Tirumalai and Soam Prakash

December 2011

Antioxidative activity of lactic acid bacteria in yogurt

  The antioxidative effect of intact cells and cell-free extract of Lactobacillus casei subsp.casei SY13 and Lactobacillus delbrueckii subsp. bulgaricus LJJ, isolated from the traditional yogurt, was evaluated by various antioxidant assays. The results showed that two Lactobacillus strains had good antioxidant capacity, inhibiting the peroxidation of linoleic acid by...

Author(s): Shuwen Zhang, Lu Liu, Yanling Su, Hongjuan Li, Qi Sun, Xiao Liang and Jiaping Lv

December 2011

Clinical features, epidemiology and outcomes of infective endocarditis at a general hospital in China: A 10-year survey

  This study retrospectively investigated the epidemiology, treatment and outcome of infective endocarditis (IE) in People's Liberation Army (PLA) General Hospital from 1997-2007. Totally, 65 cases were included. The results showed that bacteria were isolated from 63.08% of the IE cases. Staphylococcus was the main pathogen causing IE (43.9%), followed by Streptococcus (41.5%). Surgical...

Author(s): Yun Cai, Dong Chai and Rui Wang

December 2011

Variation of abundance of Planctomycetes in typical aquatic environments of the China seas

  The CARD-FISH approach with the HRP labeled oligonucleotide probe Pla-46 was applied to investigate the abundance of Planctomycetes in the China seas. Our data subtly revealed that the abundance of Planctomycetes was varied from 0.23 to 20.53×104cells/ml in 10 sampling sites of Yangtze River estuary and from 0.15 to 10.25×103 cells/ml in the 3 water depth...

Author(s): Qinglong Shu, Nianzhi Jiao, Gang Xu and Zhihua Shen

December 2011

Laboratory evaluation of the entomopathogenic fungi, Iranian isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metsch) Sorokin against the control of the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Bruchidae)

  In this study, the pathogenicity of five Iranian isolates of Beauveria bassiana andMetarhizium anisopliae was evaluated against adults of Callosobruchus maculatus adults by immersion bioassay method at 27 ± 1°C and 60 ± 5% R.H. under laboratory conditions.Cumulative mortality of the cowpea weevil at day 11 after treatment with B. bassianaisolates IRAN...

Author(s): Zahra Mahdneshin, Samad Vojoudi, Youbert Ghosta,  Mohammad Hassan Safaralizadae and Moosa Saber

December 2011

Evaluation of IgM against Toxoplasma gondii in under marriage women and its pathogenicity relation with demographic factors

  Toxoplasmosis is an acute infectious disease with worldwide spread between human societies and domestic animals. This study has been carried out to determine the immunity against toxoplasma infection in women at the time of marriage counseling in Zahedan and its relation with conducted demographic factors. This descriptive - analytical study was conducted on 280 women referred to marriage counseling of...

Author(s): Hossein Ali Khazaei, Behzad Narouie, Mohammed Bokaeian and Agheel Miri

December 2011

A comparison of fucidic acid and cefazoline released from cancellous human bone

  This study was designed to determine the antibacterial activity of fucidic acid or cefazoline in cancellous bone obtained from patients undergoing total knee replacement. Thirty samples of cancellous bone were obtained from patients undergoing total joint arthroplasty for primary osteoarthritis of knee joints. The prophylactic antibiotics were infused to the subjects an hour before the operation. In the first...

Author(s):   Yunus Dogramaci, Aydiner Kalaci, Burçin Özer, Raif Ozden, Onur Hapa and Ahmet Nedim Yanat

December 2011

Optimization and hyper-expressed production of lysine through chemical mutagenesis of Brevibacterium flavum by N-nitroso-N-ethylurea

  One of best-known commercially produced amino acids is lysine, used as a feed additive to enhance its nutritional value. Some microorganisms have the capability of producing high amounts of lysine. Brevibacterium flavum was used for lysine production in this study. The bacterium was grown on nutrient agar medium. Following the optimization of culture conditions, the maximum growth was achieved...

Author(s): Aisha Javed, Amer Jamil and Saeed Rezaei-Zarchi

December 2011

Frequency of extended spectrum beta-lactamase producing Klebsiella pneumoniae isolated from blood in Vojvodina, Serbia during 2009 to 2010

  The prevalence of extended-spectrum beta-lactamases (ESBL) varies between countries. The aim of this study was to determine the prevalence of ESBL producing Klebsiella pneumoniae originating from blood in the Vojvodina Province, Serbia. The study was conducted at Institute of Public Health of Vojvodina in Novi Sad, Serbia, in a two year period from January 2009 to December 2010. A total of 11894...

Author(s): Medić Deana, Gusman Vera, Jelesić Zora and Mihajlović-Ukropina Mira

December 2011

Study on the effect of bacterial and chemical volatiles on the growth of the fungus Aureobasidium pullulans

The aim of this study was firstly to determine if volatiles produced by a range of bacteria influences the growth of the fungus Aureobasdium pullulans and secondly, to determine if this fungus can use organic volatiles hydrocarbons as a sole carbon source. The results show that a) bacteria produce volatiles which influence the growth of the fungus A. pullulans, b) that the response to such volatiles can...

Author(s): Sulamain Ali Alharbi, Naif Abdullah Al-Harbi, Salah Hajomer, Milton Wainwright,  and Basam O. AlJohny

December 2011

Effect of calcium chloride on anthracnose disease and postharvest quality of red-flesh dragon fruit (Hylocereus polyrhizus)

  The study was conducted to examine the effects of CaCl2 postharvest treatment on development of   anthracnose, measured as lesion size and quality of red-flesh dragon fruit (Hylocereus polyrhizus). Fully matured fruits were treated with varying concentrations of Ca by soaking the fruits for 30 min in solutions containing 0, 1.0, 2.0, 3.0 and 4.0 CaCl2gL-1. After drying, the fruits were...

Author(s): Yahya Awang, Muhd Azlan Abdul Ghani, Kamaruzaman Sijam and Rosli B. Mohamad

December 2011

The chloride ion been responsible for filament formation and inhibitory effect on cell division in Zymomonas mobilis 232B growth

  To elucidate respective effects of sodium and chloride ion on filament formation inZymomonas mobilis 232B culture induced by NaCl, the morphological changes of cell grown in medium containing 0.2 M (NH4)2SO4, Na+(as Na2SO4) and Cl-(as NH4Cl) were observed using a scanning electronic nicroscope (SEM). The occurrence of filament formation began as early as 4 h after exposuring to NaCl and Cl- and the...

Author(s):   Xinhui Wang,, Yanling Jin, Yang Fang, Yuhong Huang,, Guohua Zhang, Xiaofeng Gao, and Hai Zhao,

December 2011

Antimicrobial resistance pattern of clinical isolate of Pseudomonas aeruginosa in the University of Malaya Medical Center, Malaysia

  Pseudomonas aeruginosa is considered as one of the leading causes of nosocomial infections.  The start of antimicrobial therapy is often empirical and selective pressure on panel of antibiotics; therefore, it is important to know the susceptibility pattern of pathogens in order to select the most appropriate antibiotic. The aim of the current study is to update the rational empirical antimicrobial...

Author(s): Abdelkodose M. Al-Kabsi, Mohd Yasim Bin Md Yusof and Shamala Devi Sekaran

December 2011

Composition of bacterial communities in deep-sea sediments from the South China Sea, the Andaman Sea and the Indian Ocean

  The bacterial diversity of deep-sea sediments collected from the South China Sea (B111), the Andaman Sea (ADM), and the Indian Ocean (P10) was investigated through the construction of 16S recombinant deoxyribonucleic acid (DNA) clone libraries for sequencing. The structure of the bacterial community and their phylogenetic relationships were analyzed using the Mothur software. The nutrient and heavy metal...

Author(s): Houbo Wu, Yatao Guo, Guanghua Wang, Shikun Dai, and Xiang Li

December 2011

Isolation and identification of cultivable lactic acid bacteria from traditional goat milk cake in Yunnan province of China

  Thirteen goat milk cake samples which were collected from different households in Yunnan province in China were used for isolation and identification of lactic acid bacteria (LAB). The results showed that LAB and yeasts were the dominant microflora in the samples with the LAB counts in all samples ranged from 8.32 log10 CFU/g to 10.16 log10CFU/g. The number of yeasts in most samples ranged from 1.60...

Author(s): Qiuhua Bao, Xia Chen, Hongxia Liu, Wenyi Zhang, Wenjun Liu, Jie Yu, Fang Wang and Heping Zhang

December 2011

Clinical and laboratory characteristics of 48 patients with miliary tuberculosis

  Miliary tuberculosis (TB) is a progressive form of TB, indicating disseminated hematogenous spread. Data were collected by scanning the Dicle University Hospital records between January 1990 and December 2010. Forty eight adult patients which were diagnosed miliary TB and followed by hospitalization were enrolled in this study. The mean age was 41.2 ± 14.1 years. Twenty-four...

Author(s): Ozlem Abakay, Abdurrahman Abakay, Mehmet Halis Tanrıverdi, Hadice S. Sen, Cengizhan Sezgi, Halide Kaya, Abdullah Cetin Tanrikulu and Alicem Tekin

December 2011

Isolation and characterization of bacteriocin with anti-listeria and anti-MRSA activity produced by food and soil isolated bacteria

  The search for a novel peptide-based antibiotic as replacement for currently used antibiotics is promising as it seems to develop less resistant compared to that of other type of antibiotic. Eighteen different food and soil samples were used for screening of a novel bioactive peptide producing bacteria. Among 1,265 isolated bacteria, one hundred and sixty seven isolated colonies showed antibacterial activity...

Author(s): Ratchaneewan Aunpad, Napat Sripotong, Keawta Khamlak, Siriwipa Inchidjuy, Panthip Rattanasinganchan and Duangnate Pipatsatitpong

December 2011

Screening of cytomegalovirus seroprevalence among pregnant women in Ankara, Turkey: A controversy in prenatal care

  Cytomegalovirus (CMV) seropositivity is common among pregnant women. CMV is the most common of the teratogenic viruses and is a leading cause of neurological impairment in newborns, especially sensorineural deafness. The aim of this study was to determine the seroprevalence of CMV among pregnant women in a tertiary maternity hospital setting in Ankara, Turkey. The study was conducted on 11,360 pregnant women...

Author(s): Ayla Sargin Oruç, Åževki Çelen, AyÅŸe Çitil, Sibel Saygan, Serpil Ünlü and Nuri DaniÅŸman

December 2011

Streptomyces aegyptia NEAE 102, a novel cellulolytic streptomycete isolated from soil in Egypt

  In the course of our screening programme for cellulolytic activities, a novel actinomycete, strain NEAE 102, which produced high cellulolytic activities, was isolated from a soil sample collected from Dakahliyah Province, Egypt. It developed ivory aerial mycelium and faint yellow substrate mycelium on yeast extract - malt extract agar. The colour of the substrate mycelium was not sensitive to changes in pH....

Author(s): Noura El-Ahmady El-Naggar, A. A. Sherief and Sarah Shawky Hamza

December 2011

In vitro susceptibility of Pythium insidiosum to garlic extract

  Pythium insidiosum infections are difficult to treat with conventional antifungal agents because of the lack of ergosterol in the cytoplasmic membrane of this aquatic oomycete. The effectiveness of the garlic compound allicin has been demonstrated against a range of bacteria, fungi and plant oomycetes. Therefore, we evaluated the in vitro activity ofAllium sativum extract against 17...

Author(s): R. A. Zanette, P. E. R. Bitencourt, C. Weiblen, M. B. Pilotto, A. S. Pigatto, R. B. Ceolin, M. B. Moretto, S. H. Alves, and J. M. Santurio,