African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 9 February; 6(5)

February 2012

Streptococcus thermophilus bacteriocin, from production to their application: An overview

Streptococcus thermophilus have potent antimicrobial activities toward closely related bacteria and undesirable harmful microorganisms. Certain S. thermophilus strains are able to produce substances that compete and prevent pathogenic bacteria from adhering to the receptors on epithelial cells of intestinal surfaces. The potential probiotic effects ofS. thermophilus are well known in the human...

Author(s): Shadab Gul, Tariq Masud, Shabana Maqsood, Asia Latif, Imran Irshad and Irfan ul Haque

February 2011

Detection of stx1, stx2, LT and ST toxin genes and O157 and H7 antigen genes among uropathogenic Escherichia coli isolates from Iran

Infection with Shiga like toxin-producing strains of Escherichia coli (STEC) in addition to diarrhea is associated with severe and fatal complications such as hemolytic uremia syndrome (HUS). Nonetheless, the conventional diagnostic methods of the strains are not precise, and accurate data regarding their infection incidence is not available in many countries including Iran. The aim of this study was to...

Author(s): Nazemi A., Mirinargasi M., Khataminezhad M. R., Shokouhi Mostafavi S. K. and Sharifi S. H.

February 2012

Molecular process of arbuscular mycorrhizal associations and the symbiotic stabilizing mechanisms

AM fungi are coenocytic and heterokaryotic can recognize various plant hosts. During the development of arbuscular mycorrhizal (AM) symbiosis, AM fungus at first senses the signals exuded by host plants, and then secretes corresponding Myc factors to dialogize with the plants. The host plant discriminates the Myc factors by superficial receptors located on the epidermal cells, and then initiates the symbiotic...

Author(s): Fu Qiang Song and Xiang Shi Kong

February 2012

Sequence analysis and homology modeling of glyceraldehyde-3-phosphate dehydrogenase of an antarctic psychrophilic Pseudomonas sp.

Psychrophiles are organisms that thrive in cold environments and one of the cold adaptation strategies is the ability to synthesize cold-adapted enzymes. In this work, we analyzed and compared the amino acid sequence of glyceraldehydes-3-phosphate dehydrogenase (GAPDH) of an Antarctic bacterium, Pseudomonas sp. p9, to its mesophilic and thermophilic counterparts. GAPDH...

Author(s): Kuan Chee Sian, Tien Chung Fang, See Too Wei Cun and Few Ling Ling

February 2012

Helicobacter pylori and halitosis: A comparative quasi-experimental clinical trial study

Halitosis or mouth malodor is a known problem for many years. The knowledge regarding the possible association of Helicobacter pylori infection is quite limited in literature. A comparative quasi experimental clinical trial study was conducted on 17 H. pylori positive patients and 16 H. pylori negative patients who were complaining of halitosis. All patients, regardless of H....

Author(s): Rosita Shalchi,Saeid Hosseini and Changiz Gholipouri

February 2012

Different effects of dibutyryl cAMP on monolayer permeability in human aortic and coronary arterial endothelial cells

Recent studies have demonstrated species- and/or organ-specific differences in the effects of dibutyryl cAMP (DBcAMP) on vascular permeability. In this study, we investigated the permeability of monolayers of human coronary arterial endothelial cells (HCAECs) and human aortic endothelial cells (HAECs) in the presence of DBcAMP using monolayer permeability models of endothelial cells and a double incubation chamber for...

Author(s): Yong-Shan Nan, , Shao-Yan Li, Ji-Long Kang, Shogo Suzuki, Yoshiaki Ema and Kimitoshi Nishiwaki

February 2012

Omega-3 content, antimicrobial and antioxidant activities of hexanic extract from seed and leaf of Hypericum scabrum from northwestern Iran

The hexane extracts of leaf and seed of Hypericum scabrum which were collected from northwestern Iran were obtained by Soxhlet apparatus. The fatty acids were derived to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The hexanic extract from the seed and leaf contained ω-3 (37.6 and 43.2% respectively). The...

Author(s): Ali Shafaghat

February 2012

Factors affecting extracellular and intracellular polysaccharide production in submerged cultivation of Tricholoma Mongolicum

The mycelia of Tricholoma Mongolicum M-1 was used to study the effects of various carbon and nitrogen sources, initial pH, temperature, inoculums size, rotation speed and medium capacity on the production of extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) in shake-flask cultures. The optimization of submerged culture conditions were studied using single factor test and orthogonal...

Author(s): Xiaotong Wu, Rihua Xu, Qiwei Ren, Jing Bai and Ji Zhao,

February 2012

In silico designing selective inhibitor of drugs, medicinal plants compounds and experimental ligands for pteridine reductase targeting visceral leishmaniasis

This research aimed to generate and validate the three-dimensional structure of mostly targeted proteins of leishmania using homology modeling tools and then virtual screening and docking the available chemical and medicinal plants compounds to the generated proteins as the receptor. Overall, 12 drugs and 10 medicinal plants compounds were selected for internal and comparative study. Then 18 experimental...

Author(s): Neda Adibpour, Fakher Rahim, Saeid Rezaeei, Ali Khalaj and Amar Ebrahimi

February 2012

Screening and isolating quorum sensing inhibitor from bacteria

Expression of virulence factors in many pathogens requires that quorum sensing autoinducer (QSA) reaches a certain threshold concentration; therefore, QSAs play central roles in the regulation of many disease processes. Quorum sensing inhibitors (QSIs) can competitively inhibit the quorum sensing signal system, which provides a novel target to combat pathogens for the development of new drugs. In this report, we used...

Author(s): Li-Xing Weng, Yu-Qian Zhang, Han Meng, Yu-Xiang Yang, Zhe-Xue Quan, Yuan-Yuan Zhang, and Lian-Hui Wang

February 2012

The effect of different probiotics on broiler meat quality

Probiotics are the possibility to chouse growth stimulation by using physiological potential of animals. The aim of this study was to evaluate the use of different probiotics on poultry meat. The experiment was started by fattening 700 one-day-old chicks (5 groups), provenance Arbor Acres, both sexes, initial mass 40.07 ± 0.33 g. Fattening period was 42 days. Control group was fed with complete feeding...

Author(s): S. Ivanovic, M. Baltic, J. Popov-Raljic, B. Pisinov, D. Maslic-Strizak, Z. Stojanovic and I. Pavlovic

February 2012

The gene expression of the Tvmfs transporter and defense enzyme activities from Trichoderma harzianum response to pH stress

The pH stress, one of the important environmental conditions, plays critical influence on microbial survival, colonization and ecology. It has been reported to be associated directly with its effects on microbial metabolisms, but there are few studies in literature. In current study that was investigated the impact of pH stress which is the major facilitator superfamily (MFS), a ubiquitous group of proteins involved in...

Author(s): Natthiya Buensanteai and Sutruedee Prathuangwong

February 2012

Antimicrobial resistance surveillance among hospitalized and non-hospitalized extend-spectrum beta-lactamase producing Escherichia coli from four tertiary-care hospitals in Isfahan, Iran; 2008-2011

For the first time we launched a prospective laboratory-based Antimicrobial Resistance Surveillance System in Isfahan province, Iran. In a cross-sectional study prevalence of ESBL (extend-spectrum beta-lactamase) producing Escherichia coli isolates and their antimicrobial susceptibility patterns according to CLSI (Clinical and Laboratory Standards Institute) guideline were studied in four tertiary-care...

Author(s): Sina Mobasherizadeh, Dariush Shokri, Amir Houshang Zargarzadeh, Shila Jalalpour, Sayed Amin Ebneshahidi and Mehdi Sajadi

February 2012

Establishment of a soft shell turtle, Pelodiscus sinensis, embryo primary cell culture for studies of soft shell turtle poxvirus-like virus replication and characteristics

The aim of this study was to establish a soft shell turtle primary culture for replication of the isolated poxvirus-like virus. To date, the primary culture cells have been successfully subcultured for more than 25 passages in a T-25 cell culture flask. Based on morphology studies, these cells were identified as mostly fibroblast cells. The results of this study showed that the L15 medium was the optimal culture medium...

Author(s): Pan-Chen Liu, Chi-Young Wang, Shiun-Long Lin, Cheng-Chung Lin, Shao-Wen Hung, Chen-Hsuan Chang, Ching-Yu Tu, Ming-Hui Chen, Yi-Peng Chen and Way-Shyan Wang

February 2012

A survey on the prevalence of Salmonella and coliforms in unpasteurized Iranian cheese using conventional culture method

The aim of this study is to evaluate the hygienic quality of unpasteurized cheese by microbiological tests and show the possible contamination of them with coliforms andSalmonella spp. A total of 200 samples of unpasteurized cheese were collected randomly from different markets in Sarab city (East Azarbayjan province, Iran) from February 2011 till August 2011. Based on the results of bacteriological tests all of...

Author(s): Jafar Akbarmehr and Jalil Khandaghi

February 2012

Seroprevalence of Toxoplasma infection in stored sera of HIV individual in Sokoto, Nigeria

An investigation was carried out to determine the prevalence of antibodies to Toxoplasma gondii, an obligate intracellular protozoan parasite that cause toxoplasmosis among HIV positive individuals in Sokoto, Nigeria. Twenty-two (26.1%) of the 84 HIV screened positive sera had antibody to T. gondii infection. Lower titer of 1:4 occurred in 2(9.1%) of the sera, and the sera from patients that were in...

Author(s): Alayande, M. O., Edungbola, L. D., Fabiyi, J. P., Awosan, K. I., Babatunde, S. K., Suleiman, A. I. and Idowu, D.

February 2012

Effect of Astragalus mongholicus injection liquid on the immunity function in children with congenital heart disease (CHD) after undergoing cardiopulmonary bypass surgery

The effect of the administration of Astragalus mongholicus (AM) injection liquid on the immunity function in children with congenital heart disease (CHD) after undergoing cardiopulmonary bypass surgery was examined. Sixty-two children with congenital heart disease were randomly assigned to group B (untreated control, n=21) or C (n=19) and D (n=22) (2, or 4 mg/kg daily astragalus membranac injection liquid...

Author(s): Li Tong, She Shaoyi, Zhang Ge and Huang Weizhe

February 2012

Effects of melatonin on bacterial translocation in an experimental short bowel syndrome

The most important reasons for morbidity and mortality in short bowel syndrome (SBS) are septic complications. There is no specific treatment to prevent bacterial translocation (BT) which causes these complications. Melatonin is proven to have positive effects on the gastrointestinal system. The aim of this study was to evaluate the effects of exogenously administered melatonin on BT in SBS. Fifty Sprague-Dawley rats...

Author(s): Soner Akbaba, Sevil Isik, Yusuf Ozogul, Erdal Birol Bostanci, Gulden Aydog, Mehmet Ozdemir, Fuat Atalay and Musa Akoglu

February 2012

Antimicrobial efficacy of AH-Plus, adseal and endofill against Enterococcus faecalis- An in vitro study

Antimicrobial activity is one of the desirable properties of an ideal sealer. The purpose of this study was to compare the antimicrobial effect of three different sealers (AH-Plus, adseal and endofill) on Enterococcus faecalis. The antimicrobial effect of three sealers was tested by the agar diffusion method. The freshly mixed sealers were placed in prepared wells of agar plates inoculated with E....

Author(s): Sahar Shakouie, Mahsa Eskandarinezhad, Shahriar Shahi,  Hadi mokhtari, Mohammad FroughReihani, Mohammadhossein Soroush and Armin Gosili

February 2012

Induction of immune response among formosan subterranean termites, Coptotermes formosanus Shiraki (Rhinotermitidae: Isoptera)

The antimicrobial activity from the whole body homogenates of immunized workers ofCoptotermes formosanus Shiraki with entomopathogenic fungi (Metarhizium anisopliae,strain 2049 and strain 406; Beauveria bassiana, strain 3005 and 200436) and bacteria (Bacillus thuringiensis, Escherichia coli, Ralstonia solanacearum and Staphylococcus aureus), were checked by...

Author(s): Abid Hussain and Shuo-Yang Wen

February 2012

Serogroups, pathogenicity and virulence-associated genes of avian Escherichia coli isolates collected in China

This study investigated 1,386 Escherichia coli isolates collected from visceral organs of poultry with colibacillosis from 20 provinces in China. Among these E. coli isolates, 1,122 were determined to be specific to serogroups, 236 were untypeable and the rest 28 wereautoagglutinated. These isolates were distributed among 103 serogroups, and 58.8% (815/1386) belonged to 18 O...

Author(s): Xiang Chen, Jiajun Yin, Haixia Huan, Song Gao, Xinan Jiao, and Xiufan Liu

February 2012

Clonal expansion and genetic diversity of nalidixic acid-resistant Salmonella enterica serotype Paratyphi A

The aim of this study was to understand the clonal expansion and genetic diversity of nalidixic acid -resistant (NAR) Salmonella enterica serotype Paratyphi A (SPA) from Yuxi city, China. Antimicrobial susceptibility testing was performed with 3980 SPA isolates from Yuxi city of China between 1999 and 2007. The incidence of resistance to nalidixic acid (NA) of 1999 and 2000 were 25.0 and 82.2%, respectively....

Author(s): Shukun Wang, Yunbo Yao, Congjia Chu, Desheng Shan, Biao Kan, Baowei Diaoand Xianhua Wu

February 2012

Evaluation of the insecticidal activity of Fusarium solani and Trichoderma harzianum against cockroaches; Periplaneta Americana

Microbial control is one possible option to reduce cockroaches' populations. On searching for microbial control of cockroaches, a laboratory study was carried out to investigate the effect of spore suspension and metabolites of both fungi Trichoderma harzianum andFusarium solani against cockroaches; Periplaneta americana. In this study we determined the pathogenicity of both spore suspension...

Author(s): Omar A. Abdul-Wahid and Shereen M. Elbanna

February 2012

Isolation, identification and biodegradation ability of diesel oil degrading Pseudomonas sp. strain C7 from bilge water

Some highly efficient diesel oil-degrading bacteria were isolated from bilge water. One isolated strain C7 was identified as Pseudomonas sp. based on its 16S rDNA gene sequence as well as various morphological, physiological and phylogenetic characteristics. The biodegradation potential and characteristics of strain C7 in various conditions were analyzed. GC-MS analysis showed that this strain could degrade...

Author(s): Qun Luo, , Xian-Rong Shen, Jian-Guo Zhang, Zheng-Qiu Fan and Ying He

February 2012

A study on lipid production of the mixotrophic microalgae Phaeodactylum tricornutum on various carbon sources

This study is aimed to investigate the potential use of various carbon sources (glucose, starch, and acetate sodium) for culturing Phaeodactylum tricornutum with higher microalgae oil production in mixotrophic batch cultures. Results showed P. tricornutumcould grow mixotrophically with those organic substrate in a tested range from 0.5 to 5.0 g/l. Glucose and acetate sodium exerted significant promotion...

Author(s): Haiying Wang,Ru Fu and Guofeng Pei

February 2012

Study of the prevalence of human Papillomavirus infection in Chinese women with cervical cancer

Human papillomavirus (HPV) have been recognized as oncogenic factor in cervical carcinoma. There was an increased incidence of cervical cancer in Chinese women. However, little is known about the prevalence of HPV genotypes in Chinese women with cervical cancer and risk factors of cervical cancer. Cervical exfoliated cells were prospectively collected from 792 women with cervical cancer referred for colposcopic...

Author(s): Wang Yumin, Chen Jie, Zhang Wenhui, Hong Wangdong and Yu Fangyou

February 2012

Electromagnetic exposure and magnetic nanoparticle impact on some bacteria

In this paper an experimental study focused on the biological effects of low power density microwaves and magnetic colloidal nanoparticles was carried out based on the laboratory simulation of environment electromagnetic pollution and magnetic contamination. 2 Gram positive and 1 Gram negative strains were chosen for the investigation of cell density changes induced by the supply with ferrite colloidal nanoparticles...

Author(s): Poiata Antoniea, Creanga Dorina, Nadejde Claudia and Tufescu Florin Mihai

February 2012

Detection of virulence-associated factors of Streptococcus suis serotype 2 by PCR assay in Hebei, Province of China

Streptococcus suis is the causative agent of a contagious porcine disease, characterised by pneumonia, arthritis, septicemia, pericarditis and meningitis. It also has a zoonotic character and can be isolated from a wide variety of mammals, including man. The objective of this study was to investigate the distribution of virulence-associated factors of the S. suis serotype 2 strains in Hebei Province...

Author(s): Ping Rui, Zhen-Zhen Zhang, Zeng-Jun Ma, Hai Fang, Wei-Jun Yang, Xiang-zhai Zhang, Juan Chen and Qing-hui Jia

February 2012

Evaluation of the BD MGITTM TBc identification test for rapid identification of Mycobacterium tuberculosis Complex from positive BACTEC MGIT 960 cultures in a routine laboratory work

A new immunochromatographic assay BD MGITTM TBc identification test for rapid identification of Mycobacterium tuberculosis from BACTEC MGIT 960 positive cultures was evaluated. The study tested 273 positive MGIT 960 cultures with TBc ID test. The results of the test were compared with conventional biochemical tests as gold standard. The sensitivity, specificity, and positive/negative predictive value...

Author(s): A. S. Barouni, Z. B. Alnajh, N. B. Aboguttaia and W. M. Alamri

February 2012

Bioinformatics analysis of TYR within and among species

The comprehensive bioinformatics of TYR within and among species was analyzed based on variation of nucleotide and amino acid sequence. The stop codon, TAA was used in Sus scrofa, Pan troglodytes, Canis familiaris, Equus caballus, Felis catus,Macaca mulatta, Oryctolagus cuniculus, and Homo sapiens, but thermal gravimetric analysis (TGA)...

Author(s): Weiwei WU, Xianglong LI, Rongyan ZHOU and Lanhui LI

February 2012

Apple pomace increases mycelial growth of Pleurotus ostreatus

Apple pomace is a by-product from the apple processing industry and has the potential to support the growth of microorganisms. In this study, the effect of apple pomace on thegrowth rate of Pleurotus ostreatus mycelium was investigated. The mycelial growth dramatically increased by 34.5, 20 and 26% in solid culture, liquid culture, and solid-state fermentation, respectively, by adding...

Author(s): Young-Jin Park, Hye-Ran Park, Se-Ra Kim, Dae-Eun Yoon, Eun-Suk Son, O-Chul Kwon, Woorijarang Han and Chang-Soo Lee

January 2012

Strain improvement and optimization for enhanced production of cellulase in Cellulomonas sp. TSU-03

An objective of this study was to develop high cellulase-producing bacteria by mutagenesis and optimization studies. Among 328 mutant strains of Cellulomonas sp. TSU-03, the mutant M23, NTG mutant, gave the highest value of cellulase activity 2008 U/mg protein) followed by mutant M17 (1884 U/mg protein) in CMC medium. The optimum medium and environmental conditions for cellulase production...

Author(s): Kanokphorn Sangkharak, Piyaporn Vangsirikul and Siripa Janthachat