February 2012
Streptococcus thermophilus bacteriocin, from production to their application: An overview
Streptococcus thermophilus have potent antimicrobial activities toward closely related bacteria and undesirable harmful microorganisms. Certain S. thermophilus strains are able to produce substances that compete and prevent pathogenic bacteria from adhering to the receptors on epithelial cells of intestinal surfaces. The potential probiotic effects ofS. thermophilus are well known in the human...
February 2011
Detection of stx1, stx2, LT and ST toxin genes and O157 and H7 antigen genes among uropathogenic Escherichia coli isolates from Iran
Infection with Shiga like toxin-producing strains of Escherichia coli (STEC) in addition to diarrhea is associated with severe and fatal complications such as hemolytic uremia syndrome (HUS). Nonetheless, the conventional diagnostic methods of the strains are not precise, and accurate data regarding their infection incidence is not available in many countries including Iran. The aim of this study was to...
February 2012
Molecular process of arbuscular mycorrhizal associations and the symbiotic stabilizing mechanisms
AM fungi are coenocytic and heterokaryotic can recognize various plant hosts. During the development of arbuscular mycorrhizal (AM) symbiosis, AM fungus at first senses the signals exuded by host plants, and then secretes corresponding Myc factors to dialogize with the plants. The host plant discriminates the Myc factors by superficial receptors located on the epidermal cells, and then initiates the symbiotic...
February 2012
Sequence analysis and homology modeling of glyceraldehyde-3-phosphate dehydrogenase of an antarctic psychrophilic Pseudomonas sp.
Psychrophiles are organisms that thrive in cold environments and one of the cold adaptation strategies is the ability to synthesize cold-adapted enzymes. In this work, we analyzed and compared the amino acid sequence of glyceraldehydes-3-phosphate dehydrogenase (GAPDH) of an Antarctic bacterium, Pseudomonas sp. p9, to its mesophilic and thermophilic counterparts. GAPDH...
February 2012
Helicobacter pylori and halitosis: A comparative quasi-experimental clinical trial study
Halitosis or mouth malodor is a known problem for many years. The knowledge regarding the possible association of Helicobacter pylori infection is quite limited in literature. A comparative quasi experimental clinical trial study was conducted on 17 H. pylori positive patients and 16 H. pylori negative patients who were complaining of halitosis. All patients, regardless of H....
February 2012
Different effects of dibutyryl cAMP on monolayer permeability in human aortic and coronary arterial endothelial cells
Recent studies have demonstrated species- and/or organ-specific differences in the effects of dibutyryl cAMP (DBcAMP) on vascular permeability. In this study, we investigated the permeability of monolayers of human coronary arterial endothelial cells (HCAECs) and human aortic endothelial cells (HAECs) in the presence of DBcAMP using monolayer permeability models of endothelial cells and a double incubation chamber for...
February 2012
Omega-3 content, antimicrobial and antioxidant activities of hexanic extract from seed and leaf of Hypericum scabrum from northwestern Iran
The hexane extracts of leaf and seed of Hypericum scabrum which were collected from northwestern Iran were obtained by Soxhlet apparatus. The fatty acids were derived to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The hexanic extract from the seed and leaf contained ω-3 (37.6 and 43.2% respectively). The...
February 2012
Factors affecting extracellular and intracellular polysaccharide production in submerged cultivation of Tricholoma Mongolicum
The mycelia of Tricholoma Mongolicum M-1 was used to study the effects of various carbon and nitrogen sources, initial pH, temperature, inoculums size, rotation speed and medium capacity on the production of extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) in shake-flask cultures. The optimization of submerged culture conditions were studied using single factor test and orthogonal...
February 2012
In silico designing selective inhibitor of drugs, medicinal plants compounds and experimental ligands for pteridine reductase targeting visceral leishmaniasis
This research aimed to generate and validate the three-dimensional structure of mostly targeted proteins of leishmania using homology modeling tools and then virtual screening and docking the available chemical and medicinal plants compounds to the generated proteins as the receptor. Overall, 12 drugs and 10 medicinal plants compounds were selected for internal and comparative study. Then 18 experimental...
February 2012
Screening and isolating quorum sensing inhibitor from bacteria
Expression of virulence factors in many pathogens requires that quorum sensing autoinducer (QSA) reaches a certain threshold concentration; therefore, QSAs play central roles in the regulation of many disease processes. Quorum sensing inhibitors (QSIs) can competitively inhibit the quorum sensing signal system, which provides a novel target to combat pathogens for the development of new drugs. In this report, we used...
February 2012
The effect of different probiotics on broiler meat quality
Probiotics are the possibility to chouse growth stimulation by using physiological potential of animals. The aim of this study was to evaluate the use of different probiotics on poultry meat. The experiment was started by fattening 700 one-day-old chicks (5 groups), provenance Arbor Acres, both sexes, initial mass 40.07 ± 0.33 g. Fattening period was 42 days. Control group was fed with complete feeding...
February 2012
The gene expression of the Tvmfs transporter and defense enzyme activities from Trichoderma harzianum response to pH stress
The pH stress, one of the important environmental conditions, plays critical influence on microbial survival, colonization and ecology. It has been reported to be associated directly with its effects on microbial metabolisms, but there are few studies in literature. In current study that was investigated the impact of pH stress which is the major facilitator superfamily (MFS), a ubiquitous group of proteins involved in...
February 2012
Antimicrobial resistance surveillance among hospitalized and non-hospitalized extend-spectrum beta-lactamase producing Escherichia coli from four tertiary-care hospitals in Isfahan, Iran; 2008-2011
For the first time we launched a prospective laboratory-based Antimicrobial Resistance Surveillance System in Isfahan province, Iran. In a cross-sectional study prevalence of ESBL (extend-spectrum beta-lactamase) producing Escherichia coli isolates and their antimicrobial susceptibility patterns according to CLSI (Clinical and Laboratory Standards Institute) guideline were studied in four tertiary-care...
February 2012
Establishment of a soft shell turtle, Pelodiscus sinensis, embryo primary cell culture for studies of soft shell turtle poxvirus-like virus replication and characteristics
The aim of this study was to establish a soft shell turtle primary culture for replication of the isolated poxvirus-like virus. To date, the primary culture cells have been successfully subcultured for more than 25 passages in a T-25 cell culture flask. Based on morphology studies, these cells were identified as mostly fibroblast cells. The results of this study showed that the L15 medium was the optimal culture medium...
February 2012
A survey on the prevalence of Salmonella and coliforms in unpasteurized Iranian cheese using conventional culture method
The aim of this study is to evaluate the hygienic quality of unpasteurized cheese by microbiological tests and show the possible contamination of them with coliforms andSalmonella spp. A total of 200 samples of unpasteurized cheese were collected randomly from different markets in Sarab city (East Azarbayjan province, Iran) from February 2011 till August 2011. Based on the results of bacteriological tests all of...
February 2012
Seroprevalence of Toxoplasma infection in stored sera of HIV individual in Sokoto, Nigeria
An investigation was carried out to determine the prevalence of antibodies to Toxoplasma gondii, an obligate intracellular protozoan parasite that cause toxoplasmosis among HIV positive individuals in Sokoto, Nigeria. Twenty-two (26.1%) of the 84 HIV screened positive sera had antibody to T. gondii infection. Lower titer of 1:4 occurred in 2(9.1%) of the sera, and the sera from patients that were in...
February 2012
Effect of Astragalus mongholicus injection liquid on the immunity function in children with congenital heart disease (CHD) after undergoing cardiopulmonary bypass surgery
The effect of the administration of Astragalus mongholicus (AM) injection liquid on the immunity function in children with congenital heart disease (CHD) after undergoing cardiopulmonary bypass surgery was examined. Sixty-two children with congenital heart disease were randomly assigned to group B (untreated control, n=21) or C (n=19) and D (n=22) (2, or 4 mg/kg daily astragalus membranac injection liquid...
February 2012
Effects of melatonin on bacterial translocation in an experimental short bowel syndrome
The most important reasons for morbidity and mortality in short bowel syndrome (SBS) are septic complications. There is no specific treatment to prevent bacterial translocation (BT) which causes these complications. Melatonin is proven to have positive effects on the gastrointestinal system. The aim of this study was to evaluate the effects of exogenously administered melatonin on BT in SBS. Fifty Sprague-Dawley rats...
February 2012
Antimicrobial efficacy of AH-Plus, adseal and endofill against Enterococcus faecalis- An in vitro study
Antimicrobial activity is one of the desirable properties of an ideal sealer. The purpose of this study was to compare the antimicrobial effect of three different sealers (AH-Plus, adseal and endofill) on Enterococcus faecalis. The antimicrobial effect of three sealers was tested by the agar diffusion method. The freshly mixed sealers were placed in prepared wells of agar plates inoculated with E....
February 2012
Induction of immune response among formosan subterranean termites, Coptotermes formosanus Shiraki (Rhinotermitidae: Isoptera)
The antimicrobial activity from the whole body homogenates of immunized workers ofCoptotermes formosanus Shiraki with entomopathogenic fungi (Metarhizium anisopliae,strain 2049 and strain 406; Beauveria bassiana, strain 3005 and 200436) and bacteria (Bacillus thuringiensis, Escherichia coli, Ralstonia solanacearum and Staphylococcus aureus), were checked by...
February 2012
Serogroups, pathogenicity and virulence-associated genes of avian Escherichia coli isolates collected in China
This study investigated 1,386 Escherichia coli isolates collected from visceral organs of poultry with colibacillosis from 20 provinces in China. Among these E. coli isolates, 1,122 were determined to be specific to serogroups, 236 were untypeable and the rest 28 wereautoagglutinated. These isolates were distributed among 103 serogroups, and 58.8% (815/1386) belonged to 18 O...
February 2012
Clonal expansion and genetic diversity of nalidixic acid-resistant Salmonella enterica serotype Paratyphi A
The aim of this study was to understand the clonal expansion and genetic diversity of nalidixic acid -resistant (NAR) Salmonella enterica serotype Paratyphi A (SPA) from Yuxi city, China. Antimicrobial susceptibility testing was performed with 3980 SPA isolates from Yuxi city of China between 1999 and 2007. The incidence of resistance to nalidixic acid (NA) of 1999 and 2000 were 25.0 and 82.2%, respectively....
February 2012
Evaluation of the insecticidal activity of Fusarium solani and Trichoderma harzianum against cockroaches; Periplaneta Americana
Microbial control is one possible option to reduce cockroaches' populations. On searching for microbial control of cockroaches, a laboratory study was carried out to investigate the effect of spore suspension and metabolites of both fungi Trichoderma harzianum andFusarium solani against cockroaches; Periplaneta americana. In this study we determined the pathogenicity of both spore suspension...
February 2012
Isolation, identification and biodegradation ability of diesel oil degrading Pseudomonas sp. strain C7 from bilge water
Some highly efficient diesel oil-degrading bacteria were isolated from bilge water. One isolated strain C7 was identified as Pseudomonas sp. based on its 16S rDNA gene sequence as well as various morphological, physiological and phylogenetic characteristics. The biodegradation potential and characteristics of strain C7 in various conditions were analyzed. GC-MS analysis showed that this strain could degrade...
February 2012
A study on lipid production of the mixotrophic microalgae Phaeodactylum tricornutum on various carbon sources
This study is aimed to investigate the potential use of various carbon sources (glucose, starch, and acetate sodium) for culturing Phaeodactylum tricornutum with higher microalgae oil production in mixotrophic batch cultures. Results showed P. tricornutumcould grow mixotrophically with those organic substrate in a tested range from 0.5 to 5.0 g/l. Glucose and acetate sodium exerted significant promotion...
February 2012
Study of the prevalence of human Papillomavirus infection in Chinese women with cervical cancer
Human papillomavirus (HPV) have been recognized as oncogenic factor in cervical carcinoma. There was an increased incidence of cervical cancer in Chinese women. However, little is known about the prevalence of HPV genotypes in Chinese women with cervical cancer and risk factors of cervical cancer. Cervical exfoliated cells were prospectively collected from 792 women with cervical cancer referred for colposcopic...
February 2012
Electromagnetic exposure and magnetic nanoparticle impact on some bacteria
In this paper an experimental study focused on the biological effects of low power density microwaves and magnetic colloidal nanoparticles was carried out based on the laboratory simulation of environment electromagnetic pollution and magnetic contamination. 2 Gram positive and 1 Gram negative strains were chosen for the investigation of cell density changes induced by the supply with ferrite colloidal nanoparticles...
February 2012
Detection of virulence-associated factors of Streptococcus suis serotype 2 by PCR assay in Hebei, Province of China
Streptococcus suis is the causative agent of a contagious porcine disease, characterised by pneumonia, arthritis, septicemia, pericarditis and meningitis. It also has a zoonotic character and can be isolated from a wide variety of mammals, including man. The objective of this study was to investigate the distribution of virulence-associated factors of the S. suis serotype 2 strains in Hebei Province...
February 2012
Evaluation of the BD MGITTM TBc identification test for rapid identification of Mycobacterium tuberculosis Complex from positive BACTEC MGIT 960 cultures in a routine laboratory work
A new immunochromatographic assay BD MGITTM TBc identification test for rapid identification of Mycobacterium tuberculosis from BACTEC MGIT 960 positive cultures was evaluated. The study tested 273 positive MGIT 960 cultures with TBc ID test. The results of the test were compared with conventional biochemical tests as gold standard. The sensitivity, specificity, and positive/negative predictive value...
February 2012
Bioinformatics analysis of TYR within and among species
The comprehensive bioinformatics of TYR within and among species was analyzed based on variation of nucleotide and amino acid sequence. The stop codon, TAA was used in Sus scrofa, Pan troglodytes, Canis familiaris, Equus caballus, Felis catus,Macaca mulatta, Oryctolagus cuniculus, and Homo sapiens, but thermal gravimetric analysis (TGA)...
February 2012
Apple pomace increases mycelial growth of Pleurotus ostreatus
Apple pomace is a by-product from the apple processing industry and has the potential to support the growth of microorganisms. In this study, the effect of apple pomace on thegrowth rate of Pleurotus ostreatus mycelium was investigated. The mycelial growth dramatically increased by 34.5, 20 and 26% in solid culture, liquid culture, and solid-state fermentation, respectively, by adding...
January 2012
Strain improvement and optimization for enhanced production of cellulase in Cellulomonas sp. TSU-03
An objective of this study was to develop high cellulase-producing bacteria by mutagenesis and optimization studies. Among 328 mutant strains of Cellulomonas sp. TSU-03, the mutant M23, NTG mutant, gave the highest value of cellulase activity 2008 U/mg protein) followed by mutant M17 (1884 U/mg protein) in CMC medium. The optimum medium and environmental conditions for cellulase production...
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