African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2298

Full Length Research Paper

Antibacterial effect of Royal gelly, mix from Royal jelly and rape honey (1:100), rape and oak honeydew honeys against Escherichia coli (ATCC 25922)

Dinko Dinkov*
  • Dinko Dinkov*
  • Trakia University, Faculty of Veterinary Medicine, 6000 Stara Zagora, Bulgaria.
  • Google Scholar
Todor Stoyanchev
  • Todor Stoyanchev
  • Trakia University, Faculty of Veterinary Medicine, 6000 Stara Zagora, Bulgaria.
  • Google Scholar


  •  Received: 22 May 2012
  •  Accepted: 28 March 2014
  •  Published: 29 March 2014

Abstract

The Royal jelly (RJ), RJ and rape honey (HJ), rape (RH) and oak honeydew honey (HH) at concentrations of 10 to 45% were contaminated with 50 to 100 CFU/ml Escherichia coli ATCC 25922. After plating onto ChromoCult® TBX agar up to 30 min from contamination and after enrichment in Tryptic soy broth (TSB) at 37°C for 24 h, it revealed no visible colonies. These concentrations were defined as real bactericidal concentrations (RBC). Judging by results from spectrophotometric method, percent of inhibition and pH, it was observed, follow succession from drop of antibacterial activity for tested substances that RJ > HH > HJ > RH.

 

Key words: Royal jelly, honey, antibacterial, Esherichia coli.