Abstract

This experiment was conducted in order to classify and determine the biochemical biodiversityof twenty silkworm based on total cholesterol, triglycerides, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, total lipase and lipids. Twenty studied varieties were reared under standards protocols in all rearing steps. Scientific technology of silkworm rearing was followed according to standard method. Rearing in young silkworm period was performed by chopped leaves and paraffin paper coverage and in the adult period it was performed with leaves and branches. On the 5th day of 5th instar, heamolymph was sampled using standard method. From obtained results, it is showed that among studied varieties, the highest level of total cholesterol belonged to [2029] 113 (11.53 mg/dl) and 15-1433 variety (1.00 mg/dl) remained at lower level than other varieties. Other varieties were between these two groups (P < 0.05). On the basis of these dendrograms, analyzed varieties were divided into three distinct groups. At cross 3.55, two clusters were formed which were classified into subgroups in crosses of 1.50. Frequent divisions were also observed in major groups. First group included [2029] 113 variety. Meanwhile, from obtained results, it is showed that among studied varieties, the highest level of lipids belonged to [111] 1126 (25.58 mg/dl) and [2029] 113 variety (10.53 mg/dl) remained at lower level than other varieties. Other varieties were between these two groups (P < 0.05). On the basis of these dendrograms, analyzed varieties were divided into three distinct groups. At cross 2.30, two clusters were formed. Frequent divisions were also observed in major groups. First group included M2-6-18 varieties.

Key words: Silkworm, cluster analysis, biochemical marker, cholesterol, lipase.