Abstract

The combination of Dipyridamole and Aspirin and is widely used to reduce thrombosis in patients with thrombotic diseases. A rapid, simple, precise and cost effective andreversed-phase high performance liquid chromatography (RP-HPLC) method has been developed and validated for the simultaneous determination of Aspirin and Dipyridamole in pharmaceutical formulations. Separation of both Aspirin and Dipyridamole was achieved within 5 min with required resolution, accuracy and precision thus enabling the utility of the method for routine analysis. Chromatographic separation was achieved on awaters symmetry C18 3.5 µm, 50 x 4.6 mm using a mobile phase consisting of 0.1% ortho phosphoric acid and acetonitrile in the ratio of 75:25 at a flow rate of 1.0 ml per minute. The detection was made at 227 nm and the retention time of Aspirin and Dipyridamole were1.5 and 2.8 minutes respectively. The method was found linear over the range of 4 to 80 µg/ml for Dipyridamole and 0.5 to 10 µg/ml for Aspirin.

Key words: Aspirin, Dipyridamole, high performance liquid chromatography.