African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2298

Full Length Research Paper

Simultaneous spectrophotometric determination of phenacetin and paracetamol in human plasma for the quantitative assessment of liver function reserve

Peng Yang
  • Peng Yang
  • Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China.
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Lei Shi
  • Lei Shi
  • Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China.
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Lin-Na Liu
  • Lin-Na Liu
  • Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China.
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Xin-You Liu
  • Xin-You Liu
  • Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China.
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Xiang-Rui Wang
  • Xiang-Rui Wang
  • Department of Anesthesiology, Renji Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China.
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Ping He
  • Ping He
  • Department of Anesthesiology, Renji Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China.
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Yan Zhang*
  • Yan Zhang*
  • Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China.
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  •  Received: 06 June 2013
  •  Accepted: 06 July 2014
  •  Published: 22 July 2014

Abstract

A simple, economical and reliable spectrophotometric method was developed for the simultaneous determination of phenacetin and paracetamol in human plasma. We purified the plasma samples by solid phase extraction procedure. The analytes reacted with chromogenic sodium 1,2-naphthoquinone-4-sulfonate and cetyltrimethyl ammonium bromide, and the products conformed to Beer’s law over the range 2 to 24 μg/ml for phenacetin and 1 to 16 μg/ml for paracetamol. The method was validated according to the Food and Drug Administration (FDA) guideline. There was no statistical difference between the proposed method and the modified high performance liquid chromatography (HPLC) method with regard to accuracy and precision. The proposed method is suitable for the quantitative assessment of liver function reserve in patients with liver cirrhosis.

 

Key words: Spectrophotometric method, phenacetin, paracetamol, liver function reserve. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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