African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2298

Full Length Research Paper

Gas chromatography-mass spectrometry (GC-MS) analysis, antimicrobial, phytotoxic, insecticidal and leishmanicidal activities of fixed oil from Viburnum grandiflorum

Mohammad Alam*
  • Mohammad Alam*
  • Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, 25120, Pakistan.
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Ghias Uddin
  • Ghias Uddin
  • Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, 25120, Pakistan.
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Bina S. Siddiqui
  • Bina S. Siddiqui
  • H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
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Anwar Sadat
  • Anwar Sadat
  • Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, 25120, Pakistan.
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Ashfaq Ahmad Khan
  • Ashfaq Ahmad Khan
  • Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, 25120, Pakistan.
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Abdur Rauf
  • Abdur Rauf
  • Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, 25120, Pakistan.
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  •  Received: 19 July 2014
  •  Accepted: 24 November 2014
  •  Published: 15 December 2014

Abstract

The present paper deals with the proximate chemical composition and in vitro antimicrobial, phytotoxic and insecticidal activities of fixed oil isolated from aerial parts of Viburnum grandiflorum. The fixed oil was subjected to gas chromatography-mass spectrometry (GC-MS) analysis which displayed the presence of saturated and unsaturated fatty acid. Palmitic acid, methyl ester was found in highest concentration (24.22%) among the recognized constituents of interest which was followed by myristic acid, methyl ester (11.28%), linolenic acid (7.79%) and linoleic acid (6.22%), while the concentration of the rest constituents was less than 3%. The fixed oil exhibited excellent activity against Aspergillus flavus and microsporum canis with zone of inhibition of 70 mm, while low phytotoxic effects with maximum growth regulation of 50% were observed. The oil was also assessed for leishmanicidal activity which exhibited moderate leishmanicidal (Lamina minor) effect with IC50 = 5.09 ± 0.04 against amphotericin B and pentamidine sensitive.

 

Key words: Viburnum grandiflorum, gas chromatography-mass spectrometry (GC-MS), fixed oil, phytotoxic, leishmanicidal activity.