African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2298

Full Length Research Paper

Development and validation of RP-HPLC assay for levofloxacin in rat plasma and saliva: Application to pharmacokinetic studies

Naheed W. Sheikh1*, Alok S. Tripathi1, V. Chitra1, Avijit Choudhury1 and Anil P. Dewani2
1Department of Pharmacology, SRM College of Pharmacy, SRM University, Kattankulathur- 603203, Tamil Nadu, India. 2Department of Pharmaceutical Analysis, P.W. College of Pharmacy, Dhamangaon Road, Yavatmal, 445001, Maharashtra, India.
Email: [email protected]

  •  Published: 08 October 2011

Abstract

A new, simple, specific, accurate and precise reversed phase-high performance liquid chromatography (RP-HPLC) method was developed for the determination of levofloxacin in rat plasma and saliva was developed. An HPLC system based on a Phenomenex Luna C18Column (250 × 4.6 mm) and a UV detector (λ = 296 nm) were used. A mixture of Acetonitrile: water (80:20 v/v) adjusted to pH 3.5 by orthophosphoric acid at a flow rate of 1.4 ml/min was used as mobile phase. The proteins were precipitated with methanol. The average recovery was 94.79 and 92.66%, respectively in plasma and saliva. The detection limit for levofloxacin in plasma and saliva was 1 µg/ml. The calibration curve was linear over the concentration range 1 to 16 mg/ml for plasma and saliva. The inter-day and intra-day assay coefficients of variation were found to be less than 5%. The present validated method was successfully used for pharmacokinetic studies of levofloxacin in plasma and saliva.

 

Key words: High-performance liquid chromatography (HPLC), levofloxacin, plasma, saliva, pharmacokinetics.

Abbreviation

LOFLX, Levofloxacin; HPLC, high-performance liquid chromatography.